FastQCFastQC Report
Sat 1 Jul 2023
SRR2096967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27874521
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG289928010.401183216744784No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT24791948.894122342048497No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3729661.338017611136708TruSeq Adapter, Index 4 (100% over 49bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG3158631.1331602792385205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT3038811.0901747872187653No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC2371860.8509061016689757No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2037330.7308932770539807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1792850.6431859403072792No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1747960.6270816276986428No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1688500.6057503194404669Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT1565410.5615917130916798Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1535200.5507538586941099Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1527070.5478372166467004No Hit
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1215400.4360254298181483No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC1128190.40473879353837144Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1103170.3957628545437606Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1059580.38012491766226225No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG1053850.37806927695726145No Hit
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1024600.36757582309665515No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC983530.352841937624686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC972770.34898178160622023No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG938910.3368344876670706Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC927770.33283800643605677No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG921520.3305958154402008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG848870.30453258730437016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC753190.2702073337870093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT747070.268011780363867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA735510.26386462389793175No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC718450.2577443393556431Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG706760.25355054531699395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA669490.24017991197050523Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG655900.2353044918691159No Hit
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC628870.2256074642502377TruSeq Adapter, Index 4 (100% over 38bp)
AGATCGGACGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG592870.21269244411410695TruSeq Adapter, Index 4 (97% over 49bp)
CTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC588870.21125744187675907TruSeq Adapter, Index 4 (100% over 37bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC584330.20962871433736924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG575650.2065147594823244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT570420.20463849405699205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC557660.20006083691985238No Hit
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT551750.1979406211141709No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG542430.1945970659011504No Hit
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT533660.19145082349576517Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC525470.18851265641479542No Hit
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA525150.1883978562358076Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT486460.17451779709506038Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT484570.17383975853791353No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT471950.16931232647908104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC457800.16423600606446295No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT452320.16227005299929637No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA444300.1593928735134139Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA443970.15927448582883272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC432960.1553246421705327Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAACTC430010.15426632802048867Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG429870.1542161029421815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA423820.15204566205819287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG419820.15061065982084498Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA411790.14772989282936916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT402380.14435405006600832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT396850.1423701594728749No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA389950.13989478061344984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT389600.13976921791768188No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC385150.1381727779286324Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT378520.1357942617202283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG361220.12958787704369878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC351890.1262407343250849No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC350830.12586045873218774No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG335010.12018502488347692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA322430.11567194284701789Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT320220.1148791041108832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC319880.11475712892070862TruSeq Adapter, Index 4 (100% over 38bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC315670.1132467890659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG314320.1127624758107951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA310300.1113202985622605TruSeq Adapter, Index 4 (100% over 36bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA306060.10979919619067177Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC303850.10900635745453707No Hit
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA294900.10579553994847123Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA290250.10412734984755433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)