FastQCFastQC Report
Sat 1 Jul 2023
SRR2096968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19408923
Total Bases970.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG249724012.86645322875463No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT14891437.672465906531753No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4505232.3212158655068085Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2492151.2840228177524327Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA2428191.2510689026897577No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2288981.1793441604152894No Hit
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1990291.025451025798804Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1908360.9832384826298708No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1896750.9772566978600513Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1555140.8012500229920021No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1396270.719395919083197No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1394160.7183087902404476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1234420.6360064388941107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1214040.6255061138631959No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1164790.6001311870833843No Hit
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1048690.540313339385189No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT939590.48410208026483487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT918600.4732874668007081No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA886420.45670746388143224No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA884210.45556881234471386No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG789890.4069726073930017No Hit
CACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA660490.340302241396908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT611090.31485003057614275No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT598000.3081057099355797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG580260.2989655840254506TruSeq Adapter, Index 5 (100% over 49bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA573150.29530232048424326No Hit
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG568680.29299925606382177No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT557610.2872956938414357No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG549830.28328722825063507No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT547970.28232890614280864No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG541600.2790469105369731Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC537160.2767593029247424No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC504840.2601071682339097No Hit
ACCCCTCCCACGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA498760.25697458844058474Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT470210.24226485931238945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG441920.22768908918851397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA438920.22614340836943914Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT429280.22117662067081206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC407870.21014561189201483No Hit
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG400520.20635869388528155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC379880.1957244098500468Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT367140.18916041863837577No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT362940.18699646549167104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC357200.18403906285784122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT344030.17725352406210276No Hit
GTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA342670.17655281542412218Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC341960.17618700429694115No Hit
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT334850.17252374075573385No Hit
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA320460.16510962509357166Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC316480.16305902187359905No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT311440.16046227809755337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA309220.159318474291438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACAC308190.15878779054355566No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC281960.1452733879154449No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG280380.14445932935073214No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC273350.14083728396470016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG272220.14025507752284866Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG272140.1402138593676733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC265970.13703490914977612No Hit
CGTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC262750.13537587840396914Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT255620.131702310323968TruSeq Adapter, Index 5 (100% over 38bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT254830.13129528104161164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG252660.13017723858248087TruSeq Adapter, Index 5 (100% over 37bp)
AACGATGCCGACCGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT249040.12831211706079723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA245790.12663762950679952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG242070.12472098529114675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG237500.12236639817675612No Hit
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT230200.11860524151700741Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACA219510.11309746553170416No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC213920.11021734693882808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG211610.10902717270814047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC210490.10845011853568588Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG209880.108135830102474No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC206930.10661591063038375No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG206860.10657984474460534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCTACCCCTCCCACGGCGCAGATCGGAAGAGCACACG206850.10657469247520844No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG201380.10375640111509536Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA200730.1034215036042958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCC195340.10064443039935808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCCCCCCTCCCACGGCGCAGATCGGAAGAGCACACG195130.10053623274202284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)