FastQCFastQC Report
Sat 1 Jul 2023
SRR2096969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26932125
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG281910510.467443619840617No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT20844487.739634358595914No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2685700.9972105803014059No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2671640.9919900490585128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC2170520.8059222954000103No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1969710.7313607819657751No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1871800.6950064281968096No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1720470.6388170261351453No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1646690.6114222327424962Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1462920.5431877358359208No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1411660.5241547037227846Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1353760.5026562144650673No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG1313800.48781891514316084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1244670.46215068435929213No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1189750.44175868038634164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1180320.4382572856764923No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG1149310.4267431552467546No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA1133940.42103621604310837No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT1079290.40074446409260317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC1075290.39925924894526515Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1006290.3736392876536849Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC972910.36124516724914946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG958580.35592438398381115Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC943980.3505033486960275No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC880360.32688100177761686No Hit
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT858450.3187457358080731No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT840570.31210682409947227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG811720.30139470984929706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT805730.29917060016615843No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC801310.29752943742835Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT713690.2649957996259114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA706910.2624783599511736Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG687310.25520080572921744TruSeq Adapter, Index 6 (100% over 49bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG682370.253366565022255Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT667500.24784527771202608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG640100.2376715539527609No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG634610.23563309616303948Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG633520.2352283750353899Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC626680.23268865713344192Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC608800.22604974542484116No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT602890.22385534004464927No Hit
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT555700.2063335143439294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG553770.20561689803533884No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA545190.20243111154429885Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA543630.20185187763683707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC537860.19970945478680202No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT510320.18948374849738No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC508840.18893421889286494Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA477450.17727899302413008No Hit
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT467840.1737107636326506No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA467010.17340258148957796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC465340.17278250416556437No Hit
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA453380.1683417108750238Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT449960.1670718519240498No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT448120.16638865295627434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG444470.16503339413432844No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC435490.16169908612855466No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC435340.16164339056052948Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG434370.1612832258873No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCT432160.1604626445183958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG425940.1581531349642852No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC413950.1537012025601396No Hit
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT410390.15237936107900882Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC406510.15093870238609097Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA405190.15044858138746942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC400810.14882227080113433No Hit
CTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA400750.14879999257392426TruSeq Adapter, Index 6 (100% over 37bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC395980.14702887351072372TruSeq Adapter, Index 6 (100% over 36bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA386620.14355347006595282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA347750.12912089187169598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC336310.12487317655030936Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT331260.12299809242679513No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC327660.12166139879419095No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC326150.12110073007607088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA321260.11928505455845018TruSeq Adapter, Index 6 (100% over 38bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG316620.11756220498753812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG316610.11755849194966976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC314270.11668964108847706No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT312840.1161586766733037No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC306840.11393085395229674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG306300.11373034990740613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT303860.11282436866752994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG303650.1127463948722947Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG302870.1124567779185638Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGT296320.11002473811479786No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA283810.10537972774149831Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC283210.1051569454693976No Hit
GTGGGGGGGCCGGGCTACCCCTCCCACGGCGCAGATCGGAAGAGCACACG278800.10351949576945749No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA277810.10315190502049132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA272230.10108002988995485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG269970.10024088333170887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)