FastQCFastQC Report
Sat 1 Jul 2023
SRR2096970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32077723
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG14047354.379160578199394No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT13364854.1663960998728No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6771892.1110881218096433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5801031.8084294823544678Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC5427441.6919654802181563Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4197471.3085311572769676Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3054730.9522901609942825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2349800.7325332910942587Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC2106010.6565335076931739Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1774680.5532437573577152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG1739190.5421800044847322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1736050.5412011320130172No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1708320.5325565034650371No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1631290.5085429536254803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1472820.45914106808641003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT1401980.43705720633599837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1324710.41296883821834857Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1264350.3941520412779922TruSeq Adapter, Index 7 (100% over 49bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT1149570.3583701997800779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1146640.3574567933016941No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC881550.2748168877198671Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT834150.2600402777965256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT828900.2584036279632441Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC795710.24805688358865122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT760670.23713341498709245No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC721560.22494115308620877No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG710520.22149951229393683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC706940.22038347297905153No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG687110.21420161275162827No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT639570.1993813588327326No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA629740.1963169268591789Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG613870.19136956822028794No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA613530.19126357565965638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATACTTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT612000.19078660913681436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA609840.19011324463397855Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG607540.18939623613558854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT589500.18377239556560795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA585300.18246307569898274Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA571420.17813608528261185Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT571180.178061267004519Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA540430.16847517512387022Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA533580.1663397367699696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC530330.16532657258746203Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA516350.16096840788855243No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG514040.16024828196190857No Hit
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG511830.1595593303178034Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA498580.1554287378814263Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT487040.1518312256764609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC486860.15177511196789123No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT444840.13867567844513154No Hit
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA442140.13783397281658677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCTGGTATTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG441690.13769368854516265TruSeq Adapter, Index 7 (100% over 36bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT426640.13300195902308903No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG425560.13266527677167111Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC418290.13039890643110796No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC417200.1300591067514362No Hit
CACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA416160.12973489421303375Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA415840.12963513650890995No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC414140.1291051737057521Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA412160.12848792291148595Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACCTGGTATTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA397190.1238211328154433TruSeq Adapter, Index 7 (100% over 35bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC395040.12315088574086133No Hit
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA385700.1202392077517472Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC384040.11972171466160487Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC383920.11968430552255845Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCCTAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG378870.11811000425435433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC378250.11791672370261443Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTG367280.11449690490811958Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCAGATCGGAAGAGCACACGTCTGAACTCCA363700.11338086559323428Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC360590.11241134540628085Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG355510.11082769185331515Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA352950.11002963022032454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC352240.10980829281429982Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG345180.10760738846706795TruSeq Adapter, Index 7 (100% over 36bp)
TGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC340330.10609543576394123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC335880.1047081801909693No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC326920.10191496447550219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)