FastQCFastQC Report
Sat 1 Jul 2023
SRR2096971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26066702
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG18102726.944768079981886No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT13078275.017232329582775No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7999893.0690073489158696Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5690112.182903690693207Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA5165231.9815433498261499Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3971271.5235030499830782Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2224080.8532264649359939Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1705660.654344381579227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1617210.6204122025103138Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1424430.546455781019018No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1364620.5235107993331876TruSeq Adapter, Index 8 (100% over 49bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT1251770.4802180191418155Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1225250.47004411988904465No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1158670.44450195502292544No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1063770.4080953547556572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT1042670.4000007365718916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1002850.3847245424449936No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG928640.35625527157213827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG886130.33994710953460855No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG883640.33899186786268554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG882980.3387386712749469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA754930.28961469694171516Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC696650.26725667098200606No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA696500.2671991263029746No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT636670.24424647199327323No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT593860.22782322059768054No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG565600.21698180306814419No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC565600.21698180306814419No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG560840.21515571858687763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA551340.21151122224821536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA531610.20394217879960416Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT527760.2024651987044621Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC527240.20226571048381956Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT504800.19365702650070576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA489460.18777212399175008Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA489140.1876493620098162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA479030.18377085064309248No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT457810.17563019671610164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC446340.1712299469261589No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT434480.16668008097073422No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT430220.16504581208623936No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT420640.16137062525209367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC408040.15653687221344686No Hit
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG403160.1546647519889551Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCTCCCACGGCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC402390.15436935596992668Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG397590.1525279262409184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC389420.14939365938966886Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG382700.14681565776905725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC373190.14316732511845956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA367070.140819502213974Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA362910.13922359644883345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA362250.13897039986109483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC360750.13839495307077973No Hit
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG357160.13701771708595892TruSeq Adapter, Index 8 (100% over 38bp)
GTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAACTC342120.13124790393506627Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG341110.13086043642958745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGACTGCATACTTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT339770.1303463706302393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT339090.1300855014186298TruSeq Adapter, Index 8 (100% over 36bp)
CACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC335380.12866222969058377Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT334320.12825558062542777Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGACGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG324890.12463793847031358TruSeq Adapter, Index 8 (97% over 49bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA324760.12458806641515294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC323520.12411236373515912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG319910.12272745512646747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGGTATTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT309500.11873385440168073TruSeq Adapter, Index 8 (100% over 36bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG303590.11646659404783927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC289950.11123386456790736No Hit
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC288650.11073514401630094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACAGATCGGAAGAGCACACGTCTGAACTCCAGT287530.11030547707953234Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC281810.10811110665246414Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC276900.10622747749216607No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA272150.10440522932283493Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT265950.1020267159228659Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC261620.10036559285482298Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)