FastQCFastQC Report
Sat 1 Jul 2023
SRR2096972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20450140
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG212588810.395469175272149No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT15023107.346208876809646No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1679170.8211044031972398No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1663890.8136325717085555No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1615210.7898283336935591No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1515600.7411196206969732Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG1338130.6543378187141995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1119010.547189407994273Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1050630.5137519840939965No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1044210.5106126412826514Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1042690.5098693700874419No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG984570.48144902675482903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC978270.478368363248369Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT913040.44647127110132256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT883910.43222686984049985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG815900.39897037379695205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT815540.3987943358822971Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT803670.3929899746407604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC763330.37326394831526827Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC759940.3716062579522683No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA744270.363943718722708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT731570.3577334922890503No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG676980.33103929850846986No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC672220.3287116860813667No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA665480.3254158651236618No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG636700.3113426118354202Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC629420.30778273400573297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG547780.26786124691566904TruSeq Adapter, Index 9 (100% over 49bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA468320.2290057671976818Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG454110.22205715951088842Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC450790.2204336987424047No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT409840.20040938595041405No Hit
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA406100.198580547614833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT392410.19188621691587443No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG383450.18750482881779781No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC380590.18610630538470643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT371270.18154887937197495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC352300.17227265925807844No Hit
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC352240.17224331960563596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC334590.16361257184547393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC334590.16361257184547393Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC331130.16192065188795773Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC324180.15852214214670413No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC315980.15451238964623226No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT313540.15331924378023817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG309300.1512459083409698Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG306120.1496909067615185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAACTC305040.14916279301755392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG303430.14837551234368077No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCT302330.1478376187155687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA300900.14713835699902297Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGACTGCATACTTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT299490.14644887516662478Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTG292790.1431726139772148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC289050.14134377564163375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG285020.13937312898591403Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCACACGTCTG277290.1355932037629082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA265550.1298524117683302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCTG252820.12362751550845129Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGTCCGCTGGGGGCGGGGAGCGGAGATCGGAAGAGCACACGTCTGA250730.12260551761503834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA248630.12157862977955163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGAGATCGGAAGAGCACACGTCTGA247780.1211629847032832Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA245280.11994049918484664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG242410.1185370858096815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT240070.11739283936442488No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG238870.11680604631557534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG236480.11563735015995Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC234890.11485984937022437Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC234400.1146202422086108No Hit
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT232220.11355423483653414TruSeq Adapter, Index 9 (100% over 36bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG230930.11292343230902087No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA229220.11208725221441027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA220810.10797481093038973Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG220210.10768141440596496Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT214010.10464965032024232No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA208220.10181837385954326Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)