FastQCFastQC Report
Sat 1 Jul 2023
SRR2096973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20927350
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG219105110.469796701445716No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT18292348.740877368610933No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG8709534.161793060277579TruSeq Adapter, Index 10 (100% over 49bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2259151.0795203406069092No Hit
AGATCGGACGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2129941.0177781706713942TruSeq Adapter, Index 10 (97% over 49bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2113371.0098603024272064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1747410.8349886631608875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1346060.6432061393344117No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1218910.5824483271890611Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1204520.5755721579655332Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1079350.5157604761233505No Hit
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT1019130.48698473528659864TruSeq Adapter, Index 10 (100% over 38bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC979300.4679522251981259Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT967490.4623088924302408Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT937920.44817905754909243Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT882270.42158706190702594No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA826540.39495683877796284No Hit
CTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC795870.3803013759506101TruSeq Adapter, Index 10 (100% over 37bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG768520.36723235383361963No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT742330.35471763027808106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC722170.34508430355491737No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG715660.34197354180056244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC680570.32520601031664303No Hit
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC630600.30132816625134096Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA578010.2761983719868975No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC577550.27597856393666664Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT567470.27116190057508477TruSeq Adapter, Index 10 (100% over 38bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG553110.2643000666591805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG544900.260376970806146Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC524340.25055250664799894Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT511500.24441699498503155No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA476770.22782148719259726Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC424810.2029927343882527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT407080.19452056758261319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG406830.19440110668574856Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT399040.1906787051394467TruSeq Adapter, Index 10 (100% over 39bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC395980.18921650376182364No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC395500.18898713883984355No Hit
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG388320.1855562218818914TruSeq Adapter, Index 10 (100% over 36bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG387570.1851978391912975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG386320.18460053470697435No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT386180.18453363660473018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT384840.18389332619753576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC377520.1803955111373394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA375550.17945415927004613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC368420.17604713449146692No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT357800.17097243559265746No Hit
TCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT329880.15763104263081565TruSeq Adapter, Index 10 (100% over 39bp)
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT320070.15294339703784762Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA317800.15185869209431677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT315090.15056373597230419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA314090.15008589238484565TruSeq Adapter, Index 10 (100% over 40bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG311510.1488530559292027No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA311430.148814828442206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT310560.1483991045211171TruSeq Adapter, Index 10 (100% over 39bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG305970.1462058024546825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC305780.14611501217306538No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT300540.14361111177478278No Hit
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA297020.14192910234692876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA280750.1341545871789787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT279220.13342348649016716No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC274360.13110116665511878No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT265970.12709205895634182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC261190.1248079666082901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT260670.12455948794281167No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC255200.1219456835194136No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG254840.12177365982792851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG241130.11522242424387226Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGACTGCATACTTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT240500.1149213827837734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG240390.11486881998915295Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC239650.11451521573443366Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG237290.11338750486803155No Hit
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA228640.10925415783651538Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAACTC224630.10733800505080672Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA216340.10337668171077562Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT213070.10181413317978626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)