FastQCFastQC Report
Sat 1 Jul 2023
SRR2096974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27176847
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG408280915.02311508027403No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT24448498.99607301759472No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4853501.785895177612031Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA4294851.580334171951588No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT3992101.4689341997620253No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC3201821.1781425564194405No Hit
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2666050.9810004817703835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC2423720.8918326691834413No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2357200.8673559519248132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2313570.8513018452802859Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2165480.7968106086773054Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC2098760.7722602993643818No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT2048250.753674626052095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1931330.7106527111110424No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1882600.6927220070819842No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1581770.5820285186136567No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1516630.5580595865296663Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA1489170.5479553974749167No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG1336490.49177522322585837No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1137250.4184628187368461No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1018050.3746019543768267TruSeq Adapter, Index 11 (100% over 49bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG1017010.374219275694491Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT1004980.3697927136286266No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA984440.3622348096524957No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT915670.33693018178304496No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG886890.3263402851699463No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT884990.3256411606541406No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC882300.32465134752386837No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC824260.3032949333673623No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT796820.2931981035180424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC752680.27695633713506207No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT745590.27434749881029247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG744740.27403473257953725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC733360.2698473446901327No Hit
CACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA718190.2642653873718316Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC705850.2597247576218095No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT682190.2510188175986714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT680520.2504043239453053Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA671290.24700805063957568Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT647470.23824323697300134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT596130.21935215663538896No Hit
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA588970.21671756109161597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC582440.21431478051887326Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG554840.2041590770261171No Hit
ACCCCTCCCACGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA540210.1987758182544134Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG523290.1925499304610281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG506440.18634979988664616Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC505910.18615478094276353No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG504550.18565435497355523No Hit
GCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACAC479910.17658781388437003No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCAC460630.16949353985029977No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC440190.16197243190131658No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT431250.15868286707431514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG419450.1543409358708904No Hit
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG416580.15328488989175235No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT413470.15214053344745987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA412560.1518056896004161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC407330.1498812573805931Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG393360.144740852388064Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC389400.14328372971301637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG376680.1386032750598331Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACA369420.13593188348891247No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC363640.1338050731197773No Hit
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA356030.1310048954538398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGACGAGGCGCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA349220.12849908600508367Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG348080.12807961129560028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG346080.1273436907526469Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG344120.12662248862055264No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG340840.12541557893010916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT332380.1223026350334165No Hit
GTGGGGGGGCCGGGCTACCCCTCCCACGGCGCAGATCGGAAGAGCACACG331600.12201562602166469No Hit
GGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTG320890.11807477151414952Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG312490.11498390523374548No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC310140.1141191985957753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC307880.11328760838223802Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCT301380.11089586661763963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC292060.10746647688747704Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT291370.10721258430015815No Hit
CGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCC289850.1066532846875136Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA286960.10558987950294602TruSeq Adapter, Index 11 (100% over 38bp)
GGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTC283880.10445656186679787No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCAGATCGGAAGAGCACAC276110.10159751055742411No Hit
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT275060.10121115227237361Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCGAGGGGGGCCCGGGCACGGGGGGAGATCGGAAGAGCACACGTCTGA274730.10108972538278632Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)