FastQCFastQC Report
Sat 1 Jul 2023
SRR2096975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28797113
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG384650713.357266056496705No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT26786809.301904673569188No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT3552981.2337972907214692No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC3092051.0737361068104292No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2578670.8954612915537749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA2568770.8920234469337256No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC2370580.8232005756965985No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA2319700.8055321378917393No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC2058650.7148806896024612No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1922620.6676433154948553No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA1744140.6056648803649171No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG1643870.5708454177333679Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG1558300.5411306334770433No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1428290.4959837467040533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT1367460.4748601014275285No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1324630.4599870827329115No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC1312730.4558547240482058No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1293600.4492116970197672Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT1293560.4491978067384741Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1274650.4426311762571477TruSeq Adapter, Index 12 (100% over 49bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1090910.37882616913716316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1077920.37431530028721977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1060300.3681966313775968Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT1028090.35701148236630526Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC878390.3050271046267728Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC877680.3047805521338198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC877160.30459997847700915No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC875760.3041138186317497No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG838520.29118196674784724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC831000.28857059386473916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG773990.26877347045170813No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC744700.258602311974815No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA734540.2550741805263604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG720620.2502403626363518No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT713630.2478130359803776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC712310.2473546566977044No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT709700.24644831584332777No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG705440.2449690008856096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC697100.24207287723599238No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC687470.23872879201467176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT641700.22283483764500975No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT634590.22036584014515623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG600790.20862855245246284Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC577920.2006867841231168Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG555990.19307143740415922Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT555020.19273459808280086Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA554560.1925748598479299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG548510.19047395480234425No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG535310.18589016197561192No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT533610.18529982502065398No Hit
CTCGACTGCATACTTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT512550.17798659191982197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG507520.17623988904721108No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCT504550.17520853566119632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA485840.16871135658633557Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC481490.16720078849570788No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA480150.1667354640723881Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA478940.16631528306327095No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC475380.16507904802818255No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC458770.15931110872121104Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC445540.15471689818350887No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC433840.15065399090526888No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC429500.14914689538496445No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGAGATCGGAAGAGCACACGTCTG426530.1481155419989497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG419080.14552847710810454No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC418700.14539651943581983No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA406520.14116692878206227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA401250.13933688422169266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC393400.1366109165179162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC393310.13657966338500668Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT385110.1337321557199154No Hit
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT382040.1326660766306678No Hit
CCGTCTCCGCCCCCCGGCCCCGCGTCCTAGATCGGAAGAGCACACGTCTG376780.13083950464062144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCTACCCCTCCCACGGCGCAGATCGGAAGAGCACACG374750.1301345728649952No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG374650.13009984716176237No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGT362750.12596748847705672No Hit
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT361590.12556467031955598Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA355510.12345334756300048Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA350920.12185943778461404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC342670.11899456726790633No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT339250.11780694821734386No Hit
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT337720.11727564495788172TruSeq Adapter, Index 12 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG337660.11725480953594203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG331630.11516084963100294TruSeq Adapter, Index 12 (100% over 37bp)
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG319370.11090347841465913No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG317700.11032355917067102No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC316370.10986170731767451Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGACGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG314880.10934429433950549TruSeq Adapter, Index 12 (97% over 49bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA305220.10598979140721501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG301210.10459729070757891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT297970.10347217792283551TruSeq Adapter, Index 12 (100% over 38bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC295450.10259709020136844Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA293430.10189563099606547Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT291690.1012914037598144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)