FastQCFastQC Report
Sat 30 Sept 2023
SRR21102193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21102193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60211969
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA11376081.8893386462747963No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA10353361.719485373414711No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA9551191.5862610305934357No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA5278530.8766579282600773No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA4750100.7888963073105947No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4496180.7467252897841624No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA3501420.5815156119541615No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC3370210.5597242634599775No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG3211770.5334105582894989Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2563770.4257907593090005No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2556470.4245783757711029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA2474600.4109814113536131No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG2248930.37350215203890774No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2038800.338603774940494No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT1963570.32610958130268086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1818900.3020827968605378No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA1603330.2662809449064853No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310430.21763613144755323No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA1304640.21667452861407008No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1267750.21054783974926977No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAA1182980.1964692435153549No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAGAT1127100.1871886966526539No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG1125960.18699936552481783No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA1042010.17305695483899555No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA889570.1477397292887067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT885710.14709866073305125Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG862720.14328048298835735No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA851550.14142537009543735No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA851310.14138551091062976No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA777370.12910556039115745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAA769650.12782342327984655No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT743460.12347378973771808No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA729890.12122008499672217No Hit
GGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA725930.12056240844739691Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAGA719760.11953769523796838No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCG678480.11268191545106257No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAAAA642210.10665819614701522No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA635590.10555874696607248No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA604370.10037373134235154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)