FastQCFastQC Report
Sat 30 Sept 2023
SRR21102194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21102194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59843719
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA20463073.41941816817902No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA12471312.083979774051141No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA10557041.7641015926834358No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA9553751.5964499131479446No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5726280.9568723494607679No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA4450710.7437221607166493No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA4373040.7307433550378111No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3973010.6638975762853241No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA3708140.6196372922611979No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA3493830.5838256810209272No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA3304420.5521749074451741No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA2849190.47610510302676873No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC2815960.47055230641665163No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG2734140.45688002779372716No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA2701570.45143751844700697No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA2654540.4435787154204103No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA2371350.3962571243274503No Hit
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA1966440.3285958882334836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910550.31925656224674137No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1757420.29366824611953013Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1646660.27516003809856804Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1579940.2640109983806321No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA1524320.254716789910734No Hit
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA1291750.21585389771648383No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1233070.20604835738901855No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAA1149920.192153833220158No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1086000.18147267886208743No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG1042010.17412186565477322No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAA1038080.17346515513181926No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGAT1018050.17011810378963915No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAAA999220.1669715747445442No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAG977580.16335548932044147No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA964330.1611413889567926No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAAA950930.15890222330600812No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAGA901650.15066744097237672No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAGA816790.1364871725301698No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCG797650.13328884189166118No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA769440.1285748968910171No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAAAGATCGGAA768890.12848299083818637No Hit
GAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA768540.1284245051682032No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTAAAAAAAAAA767730.1282891526176707No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA748600.12509249299830447No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTAAAAAAAAA724560.12107536298003137No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT719850.12028831296397205No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC712450.11905175879861343No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG711520.11889635401837242No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA705540.11789708457123128No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCCAAAAAAAA702240.11734564825424704No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA690560.11539389789595128No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCAAAAAAA686870.11477729183241435No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAGAA670360.11201843922835078No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCCAAAAAAAAA633600.10587577286097477No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA600480.10034135746142382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)