FastQCFastQC Report
Sat 30 Sept 2023
SRR21102195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21102195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68807252
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15490722.251320834612026No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10177601.4791464132298147No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA5193400.7547750926021577No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA4762340.6921276263147379No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA4651120.6759636324380459No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4308830.6262174225472629No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA4123730.5993161883575877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3856540.5604845256717998No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA3684710.5355118672665492No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3434190.4991029143265306No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2905300.42223746996900846No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA2751430.399875001547802No Hit
CGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA2692410.3912974173129309No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA2404810.34949949752389475No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC2263690.32899003145773065No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2145260.31177818291595194No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAAAA1857090.2698974230216315No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAA1783050.25913692934576144No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT1774880.25794955450335383No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC1674720.24339294933621242No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1654270.24042087889224237No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG1622340.23578037966114385No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1598970.23238393534448956No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCAAAAAAAAAAAAAA1414970.20564256802466113No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA1332960.19372376620999193No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1262110.18342688645667757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA1254530.18232525838991506No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1218610.17710487842182682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1154360.16776719988759325No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAAAA1077610.15661285237782785No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1057270.15365676862084246No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG996430.14481467738313397No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAAG972570.14134701964263885No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG939620.13655828022313693Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA895310.13011855203867173No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAAA894770.13004007193892878No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAAAAGATCGGAA865400.1257716265140192No Hit
GGGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCG826020.12004839257350372No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAAAA782870.11377725126996788No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT751530.10922249881451448No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAA730140.10611381486358444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)