FastQCFastQC Report
Sat 30 Sept 2023
SRR21102201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21102201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112414841
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA29897842.6595990114863928No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA27136362.4139481725549032No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA24952742.2197015783707776No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA20232061.7997677014905888No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA15669901.3939351655534522No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA15333841.364040536249124No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA11556571.0280288525249082No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9251780.823003432438249No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA7759090.6902193634735471No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6335140.5635501454830151No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA4085650.3634440046932949No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC3249890.28909794926454596Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA3176660.282583684835706No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG2807550.24974905226259228Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2762210.24571577697645813No Hit
AAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA2615690.23268191074521913No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA2571510.2287518246812269No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA2557640.22751800182682283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC2404110.213860552451433No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1895150.16858539167439643No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA1878980.16714696950022817No Hit
GAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA1848980.16447828272069523No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA1718440.15286593698068746No Hit
CCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCA1533470.13641170386034704No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAG1459290.1298129310168219No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAAA1394560.1240547945088496No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1317180.11717136174217424No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA1252220.11139276530222553No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG1238270.11015182594974271No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA1234280.10979689060806481No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA1232420.10963143202773377Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT1205870.1072696442278471No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAGA1199830.10673234862290112No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAA1193160.10613901059558498No Hit
TGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1189140.10578140656712755No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1175940.10460718438413305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1171040.10417129887680934No Hit
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA1166830.10379679316541487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)