FastQCFastQC Report
Sat 30 Sept 2023
SRR21102202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21102202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130071279
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA57672384.433905812519918No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA52831814.061758322527143No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA34732642.6702774253492194No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA33334112.5627571479480875No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA29962472.3035423523435945No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA20229131.5552341881715486No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA13666061.0506593081167441No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA8414720.6469314413368689No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8231950.6328799150195179No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA7920230.608914593666754No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5750600.4421114364532388No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA5025610.38637353600559277No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA4994420.38397562001370034No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA4109340.31592985258490464No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC3603600.2770480945297693No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA3464050.2663193617093594No Hit
AAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA3141480.24151988234082022No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC3092120.2377250399759658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAG2996380.23036446039713349No Hit
GAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA2802950.21549338343939867No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAAA2747820.21125493814818258No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAGA2458550.18901559351930414No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAAG2412540.18547830224687803No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2292850.17627642455949097No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG2284590.1756413881345781Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAA2271520.17463655446949206No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAAAA2262810.1739669216291784No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT2187350.1681654871710764No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC2115490.16264082403618096No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAA2101460.1615621846849065No Hit
CCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAA2094250.16100787322926224No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2057190.1581586662186969No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAGAA1984180.15254559002222157No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA1948940.1498363062917218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAG1927550.14819182334633613No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAG1904470.14641741164088962No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1889940.14530033182805868No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAAAAA1852270.14240422745439446No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAAA1794390.13795435962461783No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAAA1756030.13500520741400568No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA1703340.13095435157518517No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAGA1539960.11839354635699399No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGAGAAAAAAAAA1535550.11805450148606597No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAAA1531020.11770623090436437No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAGA1509190.11602792035280901No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAGAAA1496200.115029237161572No Hit
CCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCA1494440.11489392673689325No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAA1475740.11345625347468137No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG1398660.1075302719211364No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAA1394670.1072235170379158No Hit
GCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAAA1362540.10475333297829723No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGAGAAAAAAAAAA1321870.1016265858352942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)