FastQCFastQC Report
Sat 30 Sept 2023
SRR21102203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21102203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78742258
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA12145961.5424957714573033No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11589601.471839936314755No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA10189061.293976101117141No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA9036191.147565516853733No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7141970.9070059941638962No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA6828120.8671481074367972No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA6337660.8048613490357364No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA5817500.7388027912534589No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA5127640.6511929083872601No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA3945130.5010181445393654No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3725700.4731512779326191No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA3025060.3841723715873121Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2936900.3729763502590947No Hit
GGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC2215350.28134194475347657Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA2066460.2624334191686502No Hit
GGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG1464270.18595732929070943Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATC1451770.18436987163868224No Hit
TGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1373740.17446032599166764No Hit
CGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA1313440.16680243027828845No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGAT1233540.15665540096653058No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1221880.1551746204687196No Hit
AAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA1167890.14831807337808373No Hit
GGGAGAGCGACGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA1118670.1420673001274614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1041250.13223522241386573No Hit
GGGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCG995560.12643274720417594No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA993170.12612922530110832No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCG970670.12327180152745937No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA889640.11298126604395826No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGATC868270.11026734844205256No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA847760.10766264792660632No Hit
GGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA825290.10480903405132222Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)