FastQCFastQC Report
Sat 23 Sept 2023
SRR21140654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21140654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6982873
Total Bases202.7 Mbp
Sequences flagged as poor quality0
Sequence length20-147
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTCTACAGTCCGACGATC2259603.235917365244936Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGATC1426782.043256407498747Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTCTACAGTCCGACGATC1276131.827514262396008No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT842351.206308635428426No Hit
GAGTTCTACAGTCCGACGATC723721.0364215416777594Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGCTT496940.7116555034009641No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTA469650.6725741682542414No Hit
AGTCACTTAGCGATGTACACTGACTGTG391520.5606861244648156No Hit
AGAGTTCTACAGTCCGACGAT241790.3462614886451465Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGTTCTACAGTCCGACGATC232340.3327283769875236Illumina DpnII expression Sequencing Primer (95% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC201230.28817651416544454No Hit
CAGAGTTCTACAGTCCGACGAT174780.25029812227717735Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATCC171860.24611646237873724No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGCTT164440.23549046359571482No Hit
ATGTATGTAAGCGTATTACCGGTG158450.22691233250268195No Hit
TCTCGCAGCAGACGTTGATACTCGCAC154100.22068280491425235No Hit
GCAACCCCTTTACGAATCCTGTCTGACA152800.21882110701426188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC149870.21462512636274497No Hit
AGAGTTCTACAGTCCGACGA144050.2062904480720185No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC116150.1663355469876081No Hit
AGAGTTCTACAGTCCGACGATCC103530.14826275660462393Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCGTACCTGTTGGTTTGTCCC103140.14770424723462677No Hit
AGAGTTCTACAGTCGACGATC101750.14571366255694468No Hit
GAGTTCTACAGTCCGACGAT101600.14549885126079193No Hit
CCTCCCGGGGCCACGCCTGTCTGAGCGTCGCT97780.14002832358543538No Hit
GTTCAGAGTTCTACAGTCCGACGAT94540.13538839958853613Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGACGA93690.1341711355770039Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGAGTTCTACAGTCCGACGA93250.13354102244162253Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGC92770.13285362629393374No Hit
CGTTCTACAGTCCGACGATC91720.13134994722086454No Hit
GGCATTAACGCGAACTCGGCCTAC87310.12503449511397385No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCT86620.12404636315167124No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC85690.12271453311552423No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT85390.12228491052321874No Hit
CAGAGTTCTACAGTCCGACGATCC82460.1180889298717018Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCGACGATC80680.11553983582402258No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC76560.10963968555636053No Hit
ATGTATGTAAGCGTATTACCGGTGG71650.10260819579562738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)