FastQCFastQC Report
Sat 23 Sept 2023
SRR21140655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21140655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8480542
Total Bases245.6 Mbp
Sequences flagged as poor quality0
Sequence length20-147
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTCTACAGTCCGACGATC2697193.1804453064438567Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC1556491.8353661829633061No Hit
CAGAGTTCTACAGTCCGACGATC1514131.7854165453104296Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT1045591.2329282727448316No Hit
GAGTTCTACAGTCCGACGATC824990.9728033892173402Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGCTT615610.7259087921503131No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTA579600.683446883465703No Hit
AAGAGTTCTACAGTCCGACGATC486050.5731355377993529Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGTCACTTAGCGATGTACACTGACTGTG485100.5720153263789036No Hit
AGAGTTCTACAGTCCGACGAT297220.3504728825115187Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC243140.28670337344004665No Hit
CAGAGTTCTACAGTCCGACGAT215950.25464174341687124Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATCC209240.24672951327875037No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGCTT203830.24035020403177063No Hit
ATGTATGTAAGCGTATTACCGGTG196430.23162434665142864No Hit
GCAACCCCTTTACGAATCCTGTCTGACA190030.22407765918734912No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC186970.22046939924358608No Hit
TCTCGCAGCAGACGTTGATACTCGCAC184510.2175686412495805No Hit
AGAGTTCTACAGTCCGACGA179910.21214445963477335No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC140300.16543753925161858No Hit
CCTCCCGGGGCCACGCCTGTCTGAGCGTCGCT131600.15517876098013547No Hit
GAGTTCTACAGTCCGACGAT129480.15267892075765913No Hit
AGCCGTACCTGTTGGTTTGTCCC128950.15205396070204003No Hit
TAGTTCTACAGTCCGACGATC122150.14403560527145554Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGAGTTCTACAGTCGACGATC121470.14323376972839708No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC117660.13874113234743723No Hit
GAGTTCTACAGTCCGACGATCGAGTTCTACAGTCCGACGA116530.13740867034206067Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTTCAGAGTTCTACAGTCCGACGAT116240.13706671106634458Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGAGTTCTACAGTCCGACGA114890.13547483167939028Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAGTTCTACAGTCCGACGATCC113980.1344017870555915Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGTTCTACAGTCCGACGATC112490.13264482388036047No Hit
GGCATTAACGCGAACTCGGCCTAC107530.12679614109569884No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC107530.12679614109569884No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCT106800.12593534705682727No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT106740.1258645968618515No Hit
CAGAGTTCTACAGTCGACGATC97230.11465069095819584No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC92740.10935621803417753No Hit
ATGTATGTAAGCGTATTACCGGTGG89010.10495791424651867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)