FastQCFastQC Report
Sat 23 Sept 2023
SRR21140656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21140656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8545402
Total Bases254.7 Mbp
Sequences flagged as poor quality0
Sequence length20-147
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTCTACAGTCCGACGATC2270612.6571131469297757Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT2081092.435333059813921No Hit
CAGAGTTCTACAGTCCGACGATC1450491.6973923520508454Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTCTACAGTCCGACGATC1310041.5330349584489997No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGCTT758520.8876352452465079No Hit
GAGTTCTACAGTCCGACGATC682760.7989793809583211Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGTTCTACAGTCCGACGATC593590.6946308669855439Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGTCACTTAGCGATGTACACTGACTGTG571580.6688743256314916No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC501710.5871110569169244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC346210.405141852893521No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTA332010.3885247294392938No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC299910.35096066867304776No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGCTT259650.303847613020429No Hit
AGAGTTCTACAGTCCGACGAT258680.3027124996577107Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT241120.28216343713262404No Hit
CAGAGTTCTACAGTCCGACGAT234620.27455700738244965Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATCC210540.24637811070795734No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT203630.23829189077354115No Hit
GCAACCCCTTTACGAATCCTGTCTGACA200470.2345939956949948No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC195810.22914077067410052No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCT183830.21512153553454827No Hit
TCTCGCAGCAGACGTTGATACTCGCAC177950.20824064216054433No Hit
CCTCCCGGGGCCACGCCTGTCTGAGCGTCGCT149050.17442128527130732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC145810.17062977259583575No Hit
AGAGTTCTACAGTCCGACGA143940.16844146126770865No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC116830.13671679810967347No Hit
AGAGTTCTACAGTCCGACGATCC116360.1361667947277378Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGAGTTCTACAGTCCGACGA114500.13399018559922635Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTTCTACAGTCCGACGAT104200.12193691999510381No Hit
CGTTCTACAGTCCGACGATC98790.11560603000303556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC98430.11518475081687206No Hit
TAGTTCTACAGTCCGACGATC96410.11282090649451014Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGCTT96140.11250494710488752No Hit
AGAGTTCTACAGTCGACGATC95860.11217728551564923No Hit
CAGAGTTCTACAGTCCGACGATCC95830.11214217891680228Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTCGGCTGTGTTGACCTCTGCGAATTCCCCA93920.10990705879021256No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAC90640.10606873731627839No Hit
ATGTATGTAAGCGTATTACCGGTG88790.1039038303873826No Hit
CAGAGTTCTACAGTCGACGATC88140.10314318741236515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)