FastQCFastQC Report
Sat 23 Sept 2023
SRR21140657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21140657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11792221
Total Bases352.2 Mbp
Sequences flagged as poor quality0
Sequence length20-147
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTCTACAGTCCGACGATC3160662.6802923724038075Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT2840202.4085369499096054No Hit
CAGAGTTCTACAGTCCGACGATC2130501.8066995182671697Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTCTACAGTCCGACGATC1774181.5045342179390973No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGCTT1036450.8789268789992996No Hit
GAGTTCTACAGTCCGACGATC965640.8188788185024687Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCACTTAGCGATGTACACTGACTGTG776760.658705429621782No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC686200.5819090398661966No Hit
AAGAGTTCTACAGTCCGACGATC676000.5732592698186373Illumina DpnII expression Sequencing Primer (95% over 23bp)
TGCTTGGACTACATATGGTTGAGGGTTGTA475550.40327432805067004No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC472830.4009677227046542No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC409590.3473391484097864No Hit
AGAGTTCTACAGTCCGACGAT359540.3048959140097527Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGCTT347290.2945077097859682No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT328470.27854803603155No Hit
CAGAGTTCTACAGTCCGACGAT320640.27190806549504115Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTCTACAGTCCGACGATCC292300.24787527302956752No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT278600.2362574446323555No Hit
GCAACCCCTTTACGAATCCTGTCTGACA266950.22637805041136866No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC266480.22597948257584385No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCT248860.2110374288270208No Hit
TCTCGCAGCAGACGTTGATACTCGCAC239560.20315087378365787No Hit
CCTCCCGGGGCCACGCCTGTCTGAGCGTCGCT204330.17327524645272507No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC201340.1707396766054503No Hit
AGAGTTCTACAGTCCGACGA197130.16716952641915378No Hit
AGAGTTCTACAGTCCGACGATCC169620.14384058779088352Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC160090.13575898891311486No Hit
CAGAGTTCTACAGTCCGACGA155570.1319259535587062Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGAGTTCTACAGTCCGACGATCC153600.13025536071618737Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATGTATGTAAGCGTATTACCGGTG148580.12599831702611408No Hit
GAGTTCTACAGTCCGACGAT138210.11720438414442877No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC136110.11542354913463715No Hit
CGTTCTACAGTCCGACGATC131870.11182795844820073No Hit
AGAGTTCTACAGTCGACGATC129820.11008952427197558No Hit
GTCGGCTGTGTTGACCTCTGCGAATTCCCCA129690.10997928210470274No Hit
AGACCGCCTGGGAATACCAGGTGCTGTAAGCTT127260.10791860159337244No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAC121060.1026608982311305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)