FastQCFastQC Report
Sat 23 Sept 2023
SRR21218801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21218801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44955530
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCTAGGGCTT5621481.2504535037180076No Hit
CTTCCCTATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCTAGGG3608030.8025775694336159No Hit
CCTATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCTAGGGCTTA2639110.5870490237797219No Hit
CTCAAATTTCCTACGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG2576860.5732020065162172No Hit
CCTCGAGCTATTAGTATTAGTCCGCTACATGTGTCGCCACACTTCCACTT2456650.5464622483596568No Hit
GTCCGCTACATGTGTCGCCACACTTCCACTTCTAACCTATCTACCTGATC2414640.5371174580746796No Hit
GGCCTATTCACTGCGGCTGACTTAAAGTCAGCACCCCTTCTCCCGAAGTT2370780.5273611500075741No Hit
CCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT2076050.4618008062634341No Hit
CTATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCTAGGGCTTAA1975830.4395076645743026No Hit
GCCATGTTTTGTTCCAGAAGTGACTAGGACCACTTCCTTCGATAATCATC1930860.4295044458379203No Hit
CCCACATCCTTTTCCACTTAACAATTATTTTGGGACCTTAGCTGGTGGTC1669310.3713247291267615No Hit
CCCCAGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACGGAACCA1621770.36074983433628743No Hit
GTTTTGTTCCAGAAGTGACTAGGACCACTTCCTTCGATAATCATCTGTCT1575920.3505508666008387No Hit
CCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG1482450.3297592087113643No Hit
GCTTTCTCTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTCTTCCT1471130.3272411647688282No Hit
CTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGCG1313660.29221321603816036No Hit
GCCCTCCTGCCGTTTCGCTCGCCGCTACTAAGGCAATCGCTTTTGCTTTC1311920.29182616688091545No Hit
CCCAGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACGGAACCAC1287210.28632962396394834No Hit
CACGGTTTCAGGTTCTATTTCACTCCCCTCCCGGGGTGCTTTTCACCTTT1286450.28616056801020917No Hit
CTCGCATCATAACGTAACTCGCCGGTTCATTCTACAAAAGGCACGCTCTC1273730.28333110520552196No Hit
CTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC1170160.2602927826676718No Hit
GCCCTATTCAGACTCGGTTTCCCTACGGCTCCGTCTCTTCAACTTAACCT1133990.25224705392195357No Hit
CTCCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGA1059270.23562618436486013No Hit
CCCCTATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCTAGGGCT1044170.23226730949451602No Hit
CTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGAT1035040.230236413629202No Hit
GTTCTATTTCACTCCCCTCCCGGGGTGCTTTTCACCTTTCCCTCACGGTA1029230.22894402535127492No Hit
CCCTCTTGCACTCAAGTTAAACAGTTTCCAAAGCCTACTATGGTTAAGCC1021840.22730017864320584No Hit
GTCCACATTGCAGTCCTACAACCCCGAAGAGTAAACTCTTCGGTTTGCCC1018840.2266328525100249No Hit
CCCCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCA1002780.2230604332770629No Hit
CCCTTCCCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTAC1002350.22296478319797364No Hit
CTCCCCTCTTGCACTCAAGTTAAACAGTTTCCAAAGCCTACTATGGTTAA962390.21407599910400346No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATC948170.21091287323272576No Hit
CCCCATTCGGAAATCCCTGGATCATCGCTTACTTACAGCTACCCAAGGCA943420.20985627352185593No Hit
CCCCAGTCAAACTGCCCGTCAGACACTGTCTCCGATAGGGATCACCTATC938180.20869067720923323No Hit
CTTAACAATTATTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCTTT935340.20805894180315523No Hit
GTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCA919310.2044931958315251No Hit
CCCGCTACCAGGCAGGTTACCTACGCGTTACTCACCCGTTCGCAACTCAT916360.2038369918005638No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC889610.1978866671130337No Hit
CTCTTACTGATATATAATCATGGGAAATCTCATCTTGAGGTGGGTTTCAC889470.1978555252268186No Hit
GTCCCTTTCTGGTAAGATACCGTCACAGTGTGAACTTTCCACTCTCACAC886710.19724158518429213No Hit
CCCCGCTACCAGGCAGGTTACCTACGCGTTACTCACCCGTTCGCAACTCA885990.1970814269123287No Hit
CCCCGACCAAAATTTGGGTTGCTAGCTTGAGGGGTTTACCGCGTTCCACT879050.19553767912423675No Hit
CTCCATCACTACATACTCTAGTACAGGAATATCAACCTGTTGTCCATCGG866950.19284613038707363No Hit
GTCGGTTTGCGGTACGGGTAGAGTATGGTTAAACGCTAGAAGCTTTTCTT855320.1902591294107755No Hit
CTTCTATAATCTTTGAGTCCACATTGCAGTCCTACAACCCCGAAGAGTAA852710.18967855567490807No Hit
CTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGC836530.18607944339661883No Hit
CCGGCATTCTCACTTCTATGCGTTCCAGCACTCCTCACGGTATACCTTCA832180.18511182050350647No Hit
CTCCTACCATACCTATAAAGGTATCCACAGCTTCGGTAAATTGTTTTAGC832000.1850717809355156No Hit
CGTTGAGCGATGGCCCTTCCATACGGAACCACCGGATCACTAAGCCCGAC824280.18335452835279664No Hit
CCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGA823280.183132086308403No Hit
GCCCGACTTTCGTCCCTGCTCGAGTTGTAGCTCTCGCAGTCAAGCTCCCT822880.18304310949064553No Hit
TCCCTATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCTAGGGCT821340.1827005487422793No Hit
CACTTAACAATTATTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCT817620.18187306433713493No Hit
CCCGACTTTCGTCCCTGCTCGAGTTGTAGCTCTCGCAGTCAAGCTCCCTT808750.17990000340336326No Hit
CTCGAGCTATTAGTATTAGTCCGCTACATGTGTCGCCACACTTCCACTTC797560.17741087692659835No Hit
CTCACTGCTTAGACAGACTCTTCCAATCGTCTGCTTTAGTTAGCCTACTG776740.1727796335623226No Hit
GTCCACTCTGGTCCTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTAC773670.17209673648603407No Hit
CTCTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTCTTCCTCCTCA773110.17197216894117365No Hit
CCCCTCTTGCACTCAAGTTAAACAGTTTCCAAAGCCTACTATGGTTAAGC767690.17076653306056008No Hit
GTCCCGACTAACCCAGGGCGGACGAGCCTTCCCCTGGAAACCTTAGTCTT753200.1675433478372961No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCC750540.166951651999209No Hit
GTCCTCGAGCTATTAGTATTAGTCCGCTACATGTGTCGCCACACTTCCAC748210.1664333620357718No Hit
CCTCCATCACTACATACTCTAGTACAGGAATATCAACCTGTTGTCCATCG723220.16087453534637453No Hit
CTTATTTCTTACAAACCAGGTGCTTGTCCAAGTTCGGCTGCAAGCATCCA709110.15773587809998013No Hit
CCATGTTTTGTTCCAGAAGTGACTAGGACCACTTCCTTCGATAATCATCT700110.1557338997004373No Hit
CCCTATTCAGACTCGGTTTCCCTACGGCTCCGTCTCTTCAACTTAACCTC699920.1556916357120025No Hit
GCCCAATAAATCCGGACAACGCTCGGGACCTACGTATTACCGCGGCTGCT690990.15370522825556723No Hit
CACAGCTTCGGTAAATTGTTTTAGCCCCGGTACATTTTCGGCGCAGGGTC690790.1536607398466885No Hit
CCCAAATCATTCTTCTATAATCTTTGAGTCCACATTGCAGTCCTACAACC682320.15177665573067428No Hit
CTTTCTCTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTCTTCCTC680150.15129395649434008No Hit
CTTCGGTAAATTGTTTTAGCCCCGGTACATTTTCGGCGCAGGGTCACTCG675450.15024847888568993No Hit
CTCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGATAG671950.14946993173031217No Hit
CTTCGGTTTGCCCTCCTGCCGTTTCGCTCGCCGCTACTAAGGCAATCGCT669760.14898278365309006No Hit
CTTTCTGGTAAGATACCGTCACAGTGTGAACTTTCCACTCTCACACTCGT652010.1450344373651028No Hit
CCCTCCTGCCGTTTCGCTCGCCGCTACTAAGGCAATCGCTTTTGCTTTCT647230.14397116439290117No Hit
CTCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACC644870.14344620116813214No Hit
CTCCTACCTATCCTGTACATGTGGTACAGACACTCAATATCAAACTGCAG644210.14329938941883236No Hit
CCTCACTGCTTAGACAGACTCTTCCAATCGTCTGCTTTAGTTAGCCTACT638480.14202479650445674No Hit
CTATAATCTTTGAGTCCACATTGCAGTCCTACAACCCCGAAGAGTAAACT631320.1404321114665982No Hit
CTCTCTTTGGCTGATCTTCCCAAATCATTCTTCTATAATCTTTGAGTCCA626100.13927096399486336No Hit
CTTCGATAATCATCTGTCTACCACTAACAGCTCTAGCTGATAGCAGTCAG622490.1384679482146023No Hit
CCACTTAACAATTATTTTGGGACCTTAGCTGGTGGTCTGGGCTGTTTCCC616230.13707546101669807No Hit
CTCACGCGGCGTTGCTCGGTCAGACTTCCGTCCATTGCCGAAGATTCCCT615450.13690195622207102No Hit
CTGCCAACATATTTGTCTACAACCATAGGTTATGTATTATTTTAACAGCC611730.13607447181692664No Hit
GGCGTTGCTCGGTCAGACTTCCGTCCATTGCCGAAGATTCCCTACTGCTG597570.13292469246831257No Hit
CCACAGCTTCGGTAAATTGTTTTAGCCCCGGTACATTTTCGGCGCAGGGT591810.13164342629260517No Hit
GCCCCGACCAAAATTTGGGTTGCTAGCTTGAGGGGTTTACCGCGTTCCAC588400.13088489892122282No Hit
GCTCGCTTTACGCCCAATAAATCCGGACAACGCTCGGGACCTACGTATTA585810.13030877402624325No Hit
CTCTCAAATTTCCTACGCCCGCGACGGATAGGGACCGAACTGTCTCACGA576870.12832014214936405No Hit
CTCTCCTACCATACCTATAAAGGTATCCACAGCTTCGGTAAATTGTTTTA576820.12830902004714437No Hit
CTGCGTCTTCCTCCTCACATCCTTAACAGATGTGGGTAACAGGTATTACC576320.12819779902494755No Hit
GCCGAAACTTCACGGTTTGTATTGAACCCGCGCAATTAAGCAGGTGGGCA569070.12658509420309358No Hit
CTCATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTT568070.12636265215869993No Hit
CATGTTTTGTTCCAGAAGTGACTAGGACCACTTCCTTCGATAATCATCTG567640.1262670020796107No Hit
CCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTATGTATCGTCGCCTTGG564310.12552627007177983No Hit
GGGACCTTAGCTGGTGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTA555180.12349537420646582No Hit
CTCAGCGTCAGTTACAAGCCAGAGAGCCGCTTTCGCCACCGGTGTTCCTC548810.12207841838367828No Hit
CATCTGTCTACCACTAACAGCTCTAGCTGATAGCAGTCAGAATACTACGT545200.1212754026034172No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC537970.1196671466224511No Hit
CCCAGGCGGAGTGCTTAATGCGTTAGCTACGGCACTAAACCCCGGAAAGG534030.11879072496754015No Hit
CCACCAACTAGCTAATACAACGCAGGTCCATCTGGTAGTGATGCAAGTGC523010.11633941363832215No Hit
GTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTATGTATCGTCGCCTT515280.11461993663515924No Hit
CTGCGCTCGCTTTACGCCCAATAAATCCGGACAACGCTCGGGACCTACGT515160.114593243589832No Hit
CTCCCCATCACAGCTCAATGTTATAGAATTAAGCATTTGACTCAATTCAC505820.11251563489519532No Hit
CTACAACCATAGGTTATGTATTATTTTAACAGCCCCTCGACACATAACTC505690.11248671742942415No Hit
CACCGGTGTTCCTCCATATATCTACGCATTTCACCGCTACACATGGAATT505630.11247337090676053No Hit
CGCGGCGTTGCTCGGTCAGACTTCCGTCCATTGCCGAAGATTCCCTACTG504840.11229764169168954No Hit
CCCACCAACTAGCTAATACAACGCAGGTCCATCTGGTAGTGATGCAAGTG501920.1116481109220601No Hit
CCTGTGTCGGTTTGCGGTACGGGTAGAGTATGGTTAAACGCTAGAAGCTT496020.11033570286013758No Hit
ATGTTTTGTTCCAGAAGTGACTAGGACCACTTCCTTCGATAATCATCTGT495650.1102533993037119No Hit
CCCTGCTCGAGTTGTAGCTCTCGCAGTCAAGCTCCCTTATACCTTTACAC494030.10989304319179422No Hit
GTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT493400.1097529047038262No Hit
GTCACTAGGGAGTATTTAGGGTTGGGAGATGGTCCTCCCAGATTCCGACG490830.10918122864973454No Hit
GCTACTCATACCGGCATTCTCACTTCTATGCGTTCCAGCACTCCTCACGG489680.10892542029868184No Hit
CACACGGTTTCAGGTTCTATTTCACTCCCCTCCCGGGGTGCTTTTCACCT488890.10874969108361085No Hit
CTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG488300.10861845027741861No Hit
GCTGTATTGGTGCCGAAACTTCACGGTTTGTATTGAACCCGCGCAATTAA486110.10813130220019651No Hit
CCTGGTTCGGTCCTCCAGTGCGTCTTACCGCACCTTCAACCTGCTCATGG477930.10631172627705646No Hit
CTTCACTCACGCGGCGTTGCTCGGTCAGACTTCCGTCCATTGCCGAAGAT477820.10628725765217317No Hit
CCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGGTTGGG476530.10600030741490535No Hit
CCAATAAATCCGGACAACGCTCGGGACCTACGTATTACCGCGGCTGCTGG473180.10525512656618663No Hit
CCCCATCACAGCTCAATGTTATAGAATTAAGCATTTGACTCAATTCACAC470400.10463673768277228No Hit
CCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCACT469550.10444766194503767No Hit
GTTCGTTTCCGCGTACTCCATGCCCCGACCAAAATTTGGGTTGCTAGCTT469180.10436535838861202No Hit
CCCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCAC468010.10410510119667146No Hit
GTCTACAACCATAGGTTATGTATTATTTTAACAGCCCCTCGACACATAAC461980.10276377566897778No Hit
CCACTCTTATGCGGTATTAGCTATCGTTTCCAATAGTTATCCCCCGCTAC460670.10247237659082209No Hit
CCGCGTTCCACTCTCTCTGTTTCCAGAAAGACTCCGTCACTGTGGCACTT460530.10244123470460698No Hit
CTCCGTCACTGTGGCACTTTCAAGCCTACTCTGGCCTATCCAAGGACTTA456230.10148473391371428No Hit
GGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTAAA453180.10080628567831365No Hit
GCTCGAACTTGTTGTAGGCACACGGTTTCAGGTTCTATTTCACTCCCCTC451460.10042368536195657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)