FastQCFastQC Report
Sat 1 Jul 2023
SRR2126511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2126511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30971905
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG25321308.17557073095762No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT19039776.147432649041122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC13525274.366948045333343No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG10964633.5401858555358476No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC7395902.3879383589740444Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT6669702.1534677960558124No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC6518902.104778508135034Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT5629111.8174891082740956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC3113151.005152895826072Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC2806800.9062406719896628No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2319020.7487495522151446Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT1771560.5719893561600424No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1697160.5479675854617273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1668780.5388044422840635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1661320.5363958077489905No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1396230.45080533470575995No Hit
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1341710.4332022844574785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1283380.4143690870806946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1239990.40035961623929817No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1225130.3955617195648766No Hit
ACTGATTGCTCGACAATGCTCTTCCCTAAGATCGGAAGAGCACACGTCTG1199700.3873510525103316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCAGATCGGAAGAGCACACGTCTGAACTCC1170050.3777778602898337Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1162880.37546285900076215No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1134550.3663158594862021No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1099000.35483771501946687No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1085400.35044663865525866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1043880.33704094081394087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1011350.3265378735986695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT988400.31912793223406827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC959920.3099325017301971No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG921560.29754708339703356No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA913730.2950189857549931Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT911850.29441198402229374No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT910400.29394381779228623No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG889590.28722482520852366No Hit
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGTC848630.2739999363939674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT838410.27070017165556975No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC758990.24505757718164253Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG731670.23623667966177733No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC718730.2320586996505381Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC711950.22986961893367552No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG710290.22933364931863245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT708380.22871696138807088Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT675650.21814931952038466Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG644530.2081015036046378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT637680.20588982175943002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACG608830.19657492814859145No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGT596730.19266816167749448No Hit
ACTGATTGCTCGACAATGCTCTTCCCTAGATCGGAAGAGCACACGTCTGA591680.19103765170402015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC558720.18039574898605687No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG552410.17835841870236915No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT523670.16907904115035868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA513550.16581156373816852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC512730.1655468076632677No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT512450.16545640314988697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT492840.1591248584806133No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT489650.15809489277459685No Hit
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT473610.15291600565092783Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC469520.15159545400904464Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG467170.15083670184317047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTC458150.14792438501926183No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC455010.14691056297634905No Hit
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTCCCAGATCGGAAGAGCACAC449020.14497655213652502No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACG437590.14128611075101774No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACAC435290.14054350224824724No Hit
GGCCCCGGGTTCGATCCCCGGCAGATCGGAAGAGCACACGTCTGAACTCC431240.13923586553684703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC427850.13814132517841574No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC417990.13495779481436482No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC396970.12817099884556665No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC377410.1218555978393967No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC366660.11838471027209982No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT366310.11827170463037388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG360920.11653141774779434Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGGAGATCCTGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA359450.11605679405254536Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGTGTCGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA353920.11427130491327543Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC352540.11382573981161313No Hit
CCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCTGAACTC347670.11225334702531214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCTGAACTCCAG346220.11178518079530465Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCTGAACTCCA345680.11161082923378463Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGCGGTTAGGATTCCTGGAGCTCGGAAGAGCACACGTCTGAACTCC323900.1045786495858101Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTATAGTGGTTAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC320490.10347765176213733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGT319610.10319352329151209No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC312430.10087529326981985No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT311810.1006751118473339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)