FastQCFastQC Report
Sat 1 Jul 2023
SRR2126512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2126512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14418386
Total Bases735.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT237751616.489473925861052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC13181139.14189008395253No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG11889728.24622117898633No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT5691183.947168566578811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG5162703.580636556685332No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4281462.9694447076115176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT2361891.638109841143107No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2344591.626111272093839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2213441.5351510217579138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1966881.3641471382441837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1680871.1657823559446945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1208410.8381035158858973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC939310.6514668146628895Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT909150.6305490781006973No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT848010.5881448866745557No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA678620.47066294382741597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT631100.4377050246816807No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC609740.42289060647980986No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGT597050.41408934398066466No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA585450.4060440606875138Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT584750.4055585694543065No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT534870.37096385129375786No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC532210.3691189846075698No Hit
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA522960.3627035647401866Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT489910.3397814429437525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA486130.33715979028443266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC471910.32729738266127706No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC468400.32486299090619436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT422760.2932089625010733No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC419060.29064279455412No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC398790.27658435555824346No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC395330.27418464174838986Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT384500.26667339881176716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG373140.25879456965571596No Hit
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGTC363230.25192140091130866No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG356040.24693471238736428No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTC352830.24470838830365618No Hit
ATCGTATCGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT332770.2307955966777419Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC330480.22920734678624918No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA328050.227521998648115Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT315550.21885251234084035No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC309480.2146426097900278No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAAAGCACACGTCT303640.2105922257872691Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCGTATCGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC303000.21014834808833666Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT300010.20807460696363658No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC290860.20172854298671156No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT284830.19754638279208228No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG269720.1870667077438487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA222070.1540186259405179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA218420.1514871359387937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT213500.1480748261282504No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT205550.14256103283682375No Hit
ATCGTCTAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT199940.1386701673821189Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG198900.13794886612135368No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG197770.13716514455917603No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC196690.1364161009422275No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC187540.13007003696530248Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA185950.12896727830701715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT182490.12656756449716355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGAGATCGGAAGAGCACACGTC181730.12604045972968125No Hit
CTCCACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT180100.12490995871521264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCT179590.12455624367387584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC179310.12436204718059289Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGCTCGGAAGAGCACACGTCTGAACTCC179030.12416785068730994Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATCGTCTAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC173660.12044343936970477Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTC171040.1186263150397No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA162660.11281429141930312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC160280.11116362122639802No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT159850.11086539089742777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGT158660.11004005580097521No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT156310.1084101923752076No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA154280.10700226779890619Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT151230.1048869131399312No Hit
GCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCC149880.10395060861874553Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT146250.10143298979511299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)