FastQCFastQC Report
Sat 1 Jul 2023
SRR2126513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2126513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38448389
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT471112312.253108966412091Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC27002967.023170723746058No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG23435876.095410135389548No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG19767265.141245319797404No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT18412504.788887253507553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC13125403.4137711205533217Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG10417572.7094945382497038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC7004631.821826657028465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT6989291.8178368929839948No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC5110141.3290907975364066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT3672620.9552077721643941No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGT3623940.9425466435017602No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3330580.8662469577073827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG3095210.8050298284279219Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2970110.7724927044407504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC2933150.7628798179294326No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG2838240.7381947784600286No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2830110.7360802555342436No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG2422410.6300420025400804No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2278260.5925501846124165No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1895530.49300635196964954Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1860300.48384341929124786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1713610.4456909755048514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1540800.4007450091081839No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1464780.38097304935195075No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1434910.3732041932888267No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT1203190.31293638857014267No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACG1146220.29811912275440205No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1135770.29540119353245203No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGT1123650.2922489158128316No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1103180.28692489560485873No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1075270.27966581382642586Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1005100.26141537425664313No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTC1004730.2613191413559616No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG956650.24881406604578415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC882280.22947125300880616Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT834380.2170129937043656Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC829570.21576196599550632No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACAC777950.20233617590583575No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT733280.19071800381545245No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA727120.18911585606356615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT707040.18389327053469No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT702020.18258762415247098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCCTAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG653050.1698510697028164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT638450.1660537714597093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC598970.15578546086807435Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC597850.15549416127682228No Hit
GACTGCCTAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA590310.15353309081428615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA588400.15303632097563308Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTC584350.151982960846552No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT565050.14696324467586924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT564860.1469138277809247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGTC550600.14320495977087622No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG544050.14150137733989324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG528360.13742058217315686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACG517880.13469485028358405No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC516330.13429171245640487No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA511870.13313171586981187Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC499580.1299352230336621No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAAAGCACACGTCT498090.12954769054172854Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC496290.12907953048435916No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT486770.1266034839587167No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACG479460.12470223394795553No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA457110.11888924656895247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGAGATCGGAAGAGCACACGTC454380.1181792038152756No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT453970.11807256735776367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC453080.1178410882182866Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGACCAGATCGGAAGAGCA449390.11688136010067939No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT448180.11656665250655886No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC443090.11524279989988657No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG434570.11302684229500487No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG425310.1106184188887602No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT397850.10347637712466964No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA396110.10302382240254591Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCCTAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA395080.10275593081416233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGATTGCTCGACAATGCTCTTCCCTAAGATCGGAAGAGCACACGTCTG394170.10251924989627004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC389830.10139046398016831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)