FastQCFastQC Report
Thu 29 Jun 2023
SRR2126514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2126514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21734079
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT279944312.880430774177274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG21176229.743325217507492No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC18018658.290505431585116No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG14482496.663493769393219No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT10698394.922403199141772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC5389982.47996706002587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3642141.67577379285315Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT3638441.674071397274299No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3086021.4198991362827014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA2965491.3644424500343446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG2850941.3117372031269419No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGT2562741.1791343907418392No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2237551.0295122236373577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT1877550.8638737348842801No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC1765270.812212930669848No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1448120.6662900231475187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1410870.6491510406307072No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1331180.6124851207175607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1312240.603770695781496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1311230.6033059877991609Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1079330.49660719462738684No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1001570.460829281056722No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC924180.4252216070439424Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC890080.40953196130372027No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC872820.4015905159818366No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT739600.3402950730049339No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG632120.29084278197387614No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGACCAGATCGGAAGAGCAC618200.2844380937420905No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA600160.2761377650279085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT533260.24535661253462823No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTC523770.2409901979283318No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA522490.24040126107943197Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCCTAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA517890.23828476927869824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGACCAGATCGGAAGAGCAC515990.2374105661436125No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT505960.2327956938041865No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC505520.2325932467623772No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT487500.22430212018645923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC473880.21803546402863447Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT416680.19171734859342326No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT387850.17845246628578096No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC381430.17549857990301773No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC376960.17344190200100038No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA376650.17329926885790745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACAC373920.17204317698486327No Hit
ACTGATTGCTCGACAATGCTCTTCCCTAAGATCGGAAGAGCACACGTCTG372710.17148644761988766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGT356200.16389008248290624No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA344180.15835959738620625Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT342750.15770164450032595No Hit
GCGCCGCTGGTGTAGTGGTATCAGATCGGAAGAGCACACGTCTGAACTCC336820.1549732105050322Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC316940.1458262850705567Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCCTAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG316670.1457020562039919Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT311120.14314846283571528No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTC308950.1421500308340648No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAAAGCACACGTCT298050.13713486548015216Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG297130.1367115671200054No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC296110.13624225806853835No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGACCAGATCGGAAGAGCA296090.1362330559302743No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT288130.13257060490117847No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA287530.13229454075325667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGTC283580.13047711844610485No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT282120.12980536235282847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT273460.12582083648449055No Hit
GCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTC273300.12574721937837807Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGCTCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC265370.12209857155667833No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG256660.11809104034268027No Hit
GACTGCCTAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA237760.1093950196831437Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACG233640.10749937920074737No Hit
GCCGTGCTCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT225360.10368969395942657No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC223830.102985730382226No Hit
ATCGTATCGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT217480.10006405148338698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)