FastQCFastQC Report
Sat 1 Jul 2023
SRR2126515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2126515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25236826
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT332887113.190529585614291Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC18973277.5180888436604505No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT16089326.375334204071463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG11612174.601279891536281No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG9238633.6607733476468076No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG7426492.9427195004633306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACG4807081.9047878683317785No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC4404941.7454413641398485Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT3918671.5527586551494232No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGT3426841.3578728164944356No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3079561.220264386654645Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT2345670.9294631583226829No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2288550.906829567236387No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC2269080.8991146509469932No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT2011680.7971208423753446No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1793040.7104855420408256No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1671260.6622306624454279Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1671030.6621395257866421Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1542050.6110316725249046No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1541070.6106433511092084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1507460.5973255115361971Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1179600.46741218566867326No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGCAGATCGGAAGAGCACACGT1071440.4245541812587684No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1011810.40092601185267907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGACCAGATCGGAAGAGCAC1010090.40024446814349796No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA924930.36650012961217865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGACCAGATCGGAAGAGCAC923940.36610784573305694No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC908800.36010867610689234No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA811810.32167674334323976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT799750.31689801245212057No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGAGATCGGAAGAGCACACGTC782310.30998747623809747No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC644170.2552500064786277No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC593990.2353663650096094Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC591550.2343995239337942No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT582290.23073028280180718No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC579660.22968815492090802No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT548250.21724205730150062No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACAC518410.20541806643989222No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG516670.20472859780386013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT499760.19802807215138704No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT491680.19482640170360568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT471650.18688958746238535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT469100.18587915928888998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGT451220.1787942746841461No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA441930.17511314616188264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG411940.16322971834889222No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA411730.1631465066169573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGACCAGATCGGAAGAGCA405390.16063430480520807No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC401330.15902554465446644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAAAGCACACGTCT400800.15881553409291643Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACG395370.15666391645288516No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC385410.1527173028811151No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACG383400.1519208477325952No Hit
CGACTGCCTAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG369160.14627829981472312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA367280.14553335669073442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA361400.1432034281965569Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT349310.13841280991516128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACG348430.13806411313371975No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT343320.13603929432330358Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC339760.13462865734383556No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG326230.12926744432917198No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC324020.12839173991214267No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG320400.12695732815212182No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTC315220.12490477209772734No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA310390.12299090226322439Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGTC308970.12242823245680737No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGAGATCGGAAGAGCACACGTC305800.12117213155093275No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC300000.118873902764159No Hit
TCGCCTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT299650.1187352165442675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT298980.11846973149476088No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG296060.11731269217452306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCCTAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA275590.10920152954258194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT272250.1078780667584743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG268110.10623760690032891No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC264730.10489829426251938No Hit
CTCCACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT258010.10223551884060222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)