FastQCFastQC Report
Sat 23 Sept 2023
SRR21308207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21308207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38884
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCGGTTCAGCAG3660.9412611871206665Illumina Paired End PCR Primer 2 (100% over 23bp)
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCGGTTCAGCAGGA3530.9078284127147414Illumina Paired End PCR Primer 2 (100% over 25bp)
CGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCGGTTCAGCAGGA1810.46548708980557557Illumina Paired End PCR Primer 2 (100% over 25bp)
CGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCGGTTCAGCAG1450.3729040222199362Illumina Paired End PCR Primer 2 (100% over 23bp)
CGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCGGTTCAGCAGG1160.29832321777594895Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCGGTTCAGCA1120.2880362102664335Illumina Paired End PCR Primer 2 (100% over 22bp)
TTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACAGATCGGAAGAG960.24688818022837156No Hit
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCGG800.20574015019030964No Hit
TTTGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCGGTTCAG720.1851661351712787No Hit
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACAGATCGGAAGAG670.17230737578438432No Hit
ATCGGCGACGACCCATTCGAACGTCTGCCCTATCAACAGATCGGAAGAG660.16973562390700545No Hit
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCGGTTCAGCAGG600.15430511264273222Illumina Paired End PCR Primer 2 (100% over 24bp)
CTCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCGGTTC570.14658985701059563No Hit
TTCGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCGG570.14658985701059563No Hit
ATCGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCGG550.14144635325583788No Hit
TTCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCGGTTC510.1311593457463224No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCGGTTCAGC510.1311593457463224Illumina Paired End PCR Primer 2 (100% over 21bp)
TTCGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCGGTTCAG470.12087233823680692No Hit
CTTGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCGGTTC470.12087233823680692No Hit
TGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCGGTTCAGCAG460.11830058635942806Illumina Paired End PCR Primer 2 (100% over 23bp)
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCAGATCGGA430.11058533072729143No Hit
CCTGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCGGTTC430.11058533072729143No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGAGATCGGAAGAGCGGT430.11058533072729143No Hit
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAGATCGGAAGAGC430.11058533072729143No Hit
TTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCAGATCGGA430.11058533072729143No Hit
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTAGATCGGAAG400.10287007509515482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)