FastQCFastQC Report
Sat 23 Sept 2023
SRR21308208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21308208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38156
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCGGTTCAGCAG3740.9801866023692211Illumina Paired End PCR Primer 2 (100% over 23bp)
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCGGTTCAGCAGGA3300.8648705315022539Illumina Paired End PCR Primer 2 (100% over 25bp)
CGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCGGTTCAGCAGGA1660.435056085543558Illumina Paired End PCR Primer 2 (100% over 25bp)
CGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCGGTTCAGCAGG1390.36429395114791907Illumina Paired End PCR Primer 2 (100% over 24bp)
CGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCGGTTCAGCAG1180.30925673550686655Illumina Paired End PCR Primer 2 (100% over 23bp)
TTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACAGATCGGAAGAG1110.29091099695984907No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCGGTTCAGCA970.25421951986581404Illumina Paired End PCR Primer 2 (100% over 22bp)
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACAGATCGGAAGAG800.2096655833944858No Hit
CTCGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCGG760.1991823042247615No Hit
ATCGGCGACGACCCATTCGAACGTCTGCCCTATCAACAGATCGGAAGAG690.180836565677744No Hit
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCGGTTCAGCAGG670.17559492609288185Illumina Paired End PCR Primer 2 (100% over 24bp)
TTCGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCGG670.17559492609288185No Hit
CTCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCGGTTC580.1520075479610022No Hit
ATCGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCGG570.14938672816857113No Hit
TTCGGCGGCGTCCGGTGAGCTCTCGCTGGAGATCGGAAGAGCGGTTCAG540.1415242687912779No Hit
TTCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCGGTTC490.12842016982912255No Hit
TTCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTAGATCGGAAG480.1257993500366915No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGAGATCGGAAGAGCGGT480.1257993500366915No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCGGTTCAGC460.12055771045182934Illumina Paired End PCR Primer 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)