FastQCFastQC Report
Sat 23 Sept 2023
SRR21308228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21308228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95101
Total Bases4.6 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAAACAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTT2480.2607753861683894No Hit
TTCTTAATCCTATTCCATTATTCCTAGCTGCGGTATCCAGCTAGGAGAT1880.19768456693410164No Hit
CCCTTAAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTAGAT1700.17875732116381532No Hit
CCGTTAATCGAATACAGACCGTGAAAGCGGGGCCTAGATCGGAAGAGCG1650.17349975289429134No Hit
ATCTTAACACGGGAAACCTCACCCGGCCCGGACACGGATCATCGACAGA1550.16298461635524336No Hit
GTGTTAATCTCGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCGGT1510.1587785617396242No Hit
TCGTTAATCAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGATC1440.1514179661622906No Hit
ATCTTAATCCTATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT1440.1514179661622906No Hit
TCCTTAAGCTACTCAAGGACAGCAACAAGATTCCGTTCATCCAGGAGGC1350.14195434327714745No Hit
CTGTTAATCAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1330.13985131596933786No Hit
CGGTTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTATAGCAGAT1320.13879980231543307No Hit
ATGTTAATCCATCAGTAGGGTAAAACTAACCTGTCTCACGACAGATCGG1310.13774828866152827No Hit
GACTTAAATGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTG1290.13564526135371868No Hit
CCCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCAAGAT1250.1314392067380995No Hit
AAATTAATCAGTGACGCGCATGAATGGATGAACGAGATTCCCAAGATCG1240.1303876930841947No Hit
ACGTTAATATTGATCCTTCGATGTCGGCTCTTCCTAGATCGGAAGAGCG1240.1303876930841947No Hit
CGGTTAATCTCTCCTACTTGGATAACTGTAGTAATTCTAGAGAGATCGG1240.1303876930841947No Hit
ATGTTAACCAGTGATGTGATGATTCTGCCAAATGATACAAAGTGATATC1220.1282846657763851No Hit
TGGTTAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAG1220.1282846657763851No Hit
CCCTTAACATTTTCCACTATCATGAAATGCTTCTTTTATATGTTAAGTC1180.12407861116076593No Hit
ACATTAATGTGAATTGCAGGACACATTGATCATCGACACTTCGAACGCA1170.12302709750686112No Hit
CAGTTAATCAGCACTCCGGCGAGTTCTTCTGCCAGTCCTGTCAGAACGT1160.12197558385295634No Hit
GCCTTAATCTACCTGGTTGATCCTGCCAATAGCATATGCTTGTCTCAAA1160.12197558385295634No Hit
CTTTTAACCGCCCTTCTGCTCCACGGGAAGTTTCTGTCCTCCCAGATCG1150.12092407019905153No Hit
ACGTTAATCAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG1130.11882104289124193No Hit
ATCTTAAGGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTAGAT1120.11776952923733715No Hit
GCATTAATCTCGAAACTAATAGATTCTGTCCCCAGACACACGGGTTCCC1120.11776952923733715No Hit
ACGTTAATCTGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTT1120.11776952923733715No Hit
TACTTAATCGAGGGCGATCTGGCTACGACATCTGTCACCCCATTAGATC1110.11671801558343237No Hit
CCATTAAATAAAATATATACGTACACCCTCTAACCTAGAGAAGGTTAGA1110.11671801558343237No Hit
TTCTTAATCGAATGTCTGGATTTTGCTGATACTTTTATGGGGCACTTTG1110.11671801558343237No Hit
ACCTTAAGAGAAGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGC1100.11566650192952756No Hit
ATCTTAAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGAT1100.11566650192952756No Hit
TCTTTAAGCTATACTTACCTGGCAGGGGAGATACCATGATCACGAAGAG1100.11566650192952756No Hit
CCGTTAACCCGGCGGCGCGCGTCCGGCCGTGCCGGTGGTCCCGGCGGAT1080.11356347462171795No Hit
TTCTTAATCCGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTT1070.11251196096781317No Hit
ATCTTAATCAGCGGTATCTCGGTCCCTTGTGAGGACCCCCTTCCGGGAA1070.11251196096781317No Hit
ACGTTAATCATCCATGCATGTATAGAGAGGTTACTCTCAAATCGAGATC1070.11251196096781317No Hit
GTCTTAATCGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC1060.11146044731390836No Hit
AGCTTAAAGCGAATGATTAAAGGTCTTGGGGCCGAAACGATCTCAACAG1060.11146044731390836No Hit
CTGTTAATCAAGCCTCCAGCGTGCTTGGGTCTGCAGTGACCCTGTGCAG1060.11146044731390836No Hit
TTGTTAATCAAAAGCGGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGG1060.11146044731390836No Hit
TTGTTAATCAGCCCTCTCCCACGTCCGGGGAGACCCCCCATCCTTTCCG1060.11146044731390836No Hit
ATGTTAATCATGAGAAGGGGCTTCTCAGATTTTATGTTTGTTTGTTTGT1050.11040893366000358No Hit
TCGTTAATCAAGACTATACTTTCAAGGATCATTTCTATAGTTCGTTAGA1050.11040893366000358No Hit
CAATTAATGAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATT1030.10830590635219398No Hit
CCATTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAGATCGG1030.10830590635219398No Hit
CAGTTAATCCGGGGGCGGGCGGGCGTGTCCCGCGCGTGTGGGGGGAACC1030.10830590635219398No Hit
TCCTTAATTGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCAGATC1030.10830590635219398No Hit
TCATTAATCAAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGAG1030.10830590635219398No Hit
TTGTTAAACAATCAAGCTACAAATATGGGACACAGCAGGCCAGGAAAGA1020.1072543926982892No Hit
TCGTTAATCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTAG1020.1072543926982892No Hit
ACCTTAATCTTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGA1020.1072543926982892No Hit
TTTTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAGAT1020.1072543926982892No Hit
ACGTTAACCAAGACTTTGGTTTCCCGAAAGCTGCCCGGCGGGTCATGAG1020.1072543926982892No Hit
ACATTAAATGATACTGACATTTTGTAGACGTAGTCTGACAGATCGGAAG1010.10620287904438439No Hit
GCGTTAACCAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAA1010.10620287904438439No Hit
GGGTTAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCAG1010.10620287904438439No Hit
GTGTTAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTG1010.10620287904438439No Hit
CGATTAAGGGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCG1010.10620287904438439No Hit
CGGTTAATCCGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCT1000.1051513653904796No Hit
GCCTTAATCTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCT1000.1051513653904796No Hit
CGATTAATCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA1000.1051513653904796No Hit
CTGTTAATCAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGAGATC990.1040998517365748No Hit
AAGTTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGT990.1040998517365748No Hit
GGGTTAATCCCTTGCTACTGTCACCAAAACAGTTGGTGGGGACAAGAGA990.1040998517365748No Hit
AAATTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCT990.1040998517365748No Hit
GTCTTAATCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCC980.10304833808267No Hit
CCCTTAATACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCAGA980.10304833808267No Hit
GCCTTAATCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGC980.10304833808267No Hit
CTTTTAAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCC970.10199682442876522No Hit
CACTTAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCAG970.10199682442876522No Hit
GTCTTAATCAAGCGAGAGCCCCTCAGGGCTCGCCTCCCCGCCTCACCGA970.10199682442876522No Hit
CCGTTAATCGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAG970.10199682442876522No Hit
TGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGAGATCG970.10199682442876522No Hit
TAATTAATTAATGTGAATTGCAGGACACATTGATCATCGACACTTCAGA970.10199682442876522No Hit
CTCTTAACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGAGAT960.10094531077486041No Hit
ATGTTAACCACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGG960.10094531077486041No Hit
TGGTTAATCAGACATGCATGGCTTAATCTTTGAGACAAGCATATGCAGA960.10094531077486041No Hit
CCGTTAATCAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGAGATC960.10094531077486041No Hit
CAGTTAATCTATCTCTGCCTAGCTCTACCATACTAAGGCAGAGACGCTT960.10094531077486041No Hit
ATGTTAACCACTATGGCAAAGAGCAGCGCCGTGCAGTGGTGATGATCTC960.10094531077486041No Hit
TTTTTAAGCCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA960.10094531077486041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)