FastQCFastQC Report
Sat 1 Jul 2023
SRR2148109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2148109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28840018
Total Bases801.9 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAA984350.3413139339926903No Hit
AAAAAAAAAAAAAAAAAAAAAAA921240.3194311459861086No Hit
AGCTTGGGCTGCAGGTCGACCCGTGC718060.24898042712733398No Hit
AAAAAAAAAAAAAAAAAAAAAAAA716420.24841177283592542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA515990.1789145901365249No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG442690.15349851723393515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA402130.1394347257342211No Hit
AATACAAGCTTGGGCTGCAGGTCGAC374950.13001032107538907No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA299600.10388343030853864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)