FastQCFastQC Report
Thu 28 Sept 2023
SRR2163085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157559084
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA47385023.007444496186586No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG42370362.6891727804155043No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG34758032.20603148467149No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA27785741.7635124103666406No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA27240011.7288758799841715No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA26508691.6824602762986358No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA23869471.5149535903623303No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA20216521.2831072310626026No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC12457330.7906449875019584No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA11968240.7596032990392353No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA11646800.7392020633986423No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA9522300.6043637572810464RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG9491030.6023791049711865No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG9430730.5985519692409483No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA9279200.5889346246770513RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG8862690.5624994621065453Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC8671190.550345291420963No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA7856350.4986288191419036No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA7697680.48855831124278426RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA7365960.467504621948678RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA7233320.45908619270723866No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG7121680.4520005968046882No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC7076060.44910517504658765No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG6714860.4261804416176982No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC6678100.42384734859210016No Hit
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT6596510.41866897372924555RNA PCR Primer, Index 1 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA6571600.41708797951630644RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG6554980.41603313713095713No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6519210.41376287767704967No Hit
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA6421130.4075379113018961RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG6248670.39659217617690645Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA6102480.38731375209061253RNA PCR Primer, Index 1 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA6009310.38140041484374204No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5788740.3674012220076121No Hit
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG5644910.3582725830013076Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG5580690.35419665171447684Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA5561380.35297107972524133No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG5409420.3433264438120242Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA5350280.3395729312566961No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG5303660.33661404124436267Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA5252050.33333844464340756RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC5221110.33137473685744456RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA5114130.32458490301961895No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA4977310.3159011764754865No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA4938620.31344558971921926RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA4935970.31327739884550226No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4571320.29013369993950966No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA4547190.2886022109648721RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG4489390.2849337458702159Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC4452340.2825822470508904No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG4398860.27918796481452No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA4382290.2781362958418824No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA4321540.2742805993972394RNA PCR Primer, Index 1 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG4281000.2717075963706415No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC4113070.2610493724373264No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA4113040.26104746838969944RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA4079130.258895259888665No Hit
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG4064660.2579768742499163No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA4030760.2558253004314242No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG3656440.23206786350700032No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGGGCCAAG3633190.23059222659608758No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGA3527510.2238849014887647RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA3473900.22048236837934396RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA3453850.21920982988197624RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC3434510.21798235384511375RNA PCR Primer, Index 1 (100% over 24bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG3430400.21772149932021692No Hit
GCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCA3409850.21641722669573277No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG3371910.2140092411301401No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA3337630.21183354937503954RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA3260430.2069338001482669No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3249160.2062185129230632No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA3201720.20320757894225888No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3185620.2021857400491107No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA2845520.18060018678453346RNA PCR Primer, Index 1 (100% over 23bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2784590.17673306605412864Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC2769450.17577215668504395RNA PCR Primer, Index 1 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC2740640.1739436362805968No Hit
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG2726050.17301763445134016Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGGGCC2712920.17218429627326343No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA2707590.17184601047820258RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC2647660.16804235800203055RNA PCR Primer, Index 1 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA2616520.16606595656522097No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA2514500.1595909252683901RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG2502680.15884073050335834Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG2297160.1457967348934321No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA2290690.14538609528854585No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2264960.14375305710713576Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA2231770.14164654574914892RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC2224720.14119909455680765RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2173510.13794888525754567RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2093320.13285936595061698Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA2088570.13255789174301114RNA PCR Primer, Index 1 (100% over 22bp)
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC2024790.12850988648804282No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC2015590.12792597854910098No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA1969910.12502674869574643No Hit
CATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAA1966590.12481603409169349RNA PCR Primer, Index 1 (100% over 23bp)
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG1957780.12425687877190249Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCACATTGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA1900300.1206087235186008No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGA1894180.12022029780269602RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC1887560.11980013795967485RNA PCR Primer, Index 1 (100% over 26bp)
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA1837840.11664449635921975RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1799930.11423841484125409No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC1797770.11410132341211122No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGTGGAATTCTCGGGTGCCA1769370.11229882499189955No Hit
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA1709200.10847994013471161RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1705690.10825716656235448No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG1702830.10807564735524865No Hit
TCCCATATTGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA1698360.10779194425882802No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC1675110.10631630734791526No Hit
TATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCA1616110.10257168034817972No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGTGGAATTCTCGGGTGCCAA1613990.10243712764920619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)