FastQCFastQC Report
Thu 28 Sept 2023
SRR2163087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134938187
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG35418762.6248136859879407No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA33837442.5076252136098436No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG28012982.0759860957669454No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA21435661.5885540243696916No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA20075971.4877901094076504No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA19873161.4727602646684441No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA19321401.4318704311626775No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA14351261.0635432651840802No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA10778300.7987583233202918No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA10082980.7472295444431901No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA10021240.7426541161398589RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC9591490.7108062004716278No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC8283380.6138647764698365No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG8078410.5986748584372191Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA7581500.5618498490720051RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC7490470.5551037972668182No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6696270.49624721873579053No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA6570830.4869511104369588RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG6187940.45857589593967196No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA6157430.4563148606702415No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA6074810.4501920571972707No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC6040980.447684983347227No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG5830810.4321097036823238No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA5813730.43084393893627754No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5769550.42756984722197283No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5672180.4203539506574221RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA5608190.4156117793401211RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG5604680.41535166023832826No Hit
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT5442550.4033365291916957RNA PCR Primer, Index 1 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA5307800.3933504753550601RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA5011440.3713878266350207RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA4759370.35270742150996887No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA4737780.3511074296559209RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA4722840.3500002560431615No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG4653550.3448653122929538Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA4639620.3438329877664652RNA PCR Primer, Index 1 (100% over 22bp)
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4599580.34086570319786497Illumina Small RNA Adapter 2 (100% over 21bp)
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG4551220.33728184001760747No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC4547090.33697577395196515RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4496730.33324369475929No Hit
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4430040.32830143182522525RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG4386420.3250688405943975Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC4382110.3247494350876376RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA4371720.3239794529031282No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA4296110.3183761465536809No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA4292050.3180752680484732No Hit
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4273130.31667314457100276Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4265870.31613512044592684Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA4124920.30568959697079673No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA3833010.28405672887838634No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG3825200.2834779453498957No Hit
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA3593990.2663434332343594RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC3543390.2625935681201942No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG3499950.25937431633048397Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA3393790.2515070103913579RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA3267600.2421553210878697RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA3243000.2403322641351332No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG3201350.23724566567653677No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC3172520.2351091318575371No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA3060750.2268260800035797RNA PCR Primer, Index 1 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA3018960.22372910642411403No Hit
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG2975650.22051948867521098No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG2947050.21839999969763932No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA2906310.2153808395246929No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG2904430.21524151647302034No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2705940.20053181832063596No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA2701640.20021315389393812RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA2700800.20015090316872272RNA PCR Primer, Index 1 (100% over 23bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA2648600.19628246524462345No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2563860.19000255279848988No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2506540.18575468188260155No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC2433130.18031441314681365RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA2331220.17276206623407503RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC2241040.16607900623416558No Hit
GCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCA2222640.1647154189199237No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG2120190.15712305368383228Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2097270.15542449818152662Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC2012140.14911568361297162No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGA2008580.1488518591108683RNA PCR Primer, Index 1 (100% over 22bp)
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG2007440.14876737598379025Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1964840.14561037491929546Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG1912660.14174341915532035Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC1886750.13982328071445038RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA1863530.13810249281028208RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG1854650.13744441371514796No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG1844200.136669985050266Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA1820220.134892875061379RNA PCR Primer, Index 1 (100% over 23bp)
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA1799700.1333721787739745RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA1795870.1330883451101948RNA PCR Primer, Index 1 (100% over 22bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC1757030.13020999014904505No Hit
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC1714330.12704557828392937RNA PCR Primer, Index 1 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC1709850.12671357441611394No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA1708590.12662019832829086No Hit
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC1706910.12649569687786008No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGGGCCAAG1682990.12472303336934563No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA1558670.11550992603746782No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA1553980.11516235948834855RNA PCR Primer, Index 1 (100% over 22bp)
CATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAA1518710.11254857010936423RNA PCR Primer, Index 1 (100% over 23bp)
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC1507690.11173189988094327RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1480010.10968059026908372No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAG1470050.10894247452724408No Hit
TCCCACATTGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA1442010.10686448603314938No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA1429120.10590923383311797No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1419980.10523188665636955No Hit
TATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCA1408740.10439891266658266No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGTGGAATTCTCGGGTGCCA1390300.10303236103209241No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG1382610.10246247046434676No Hit
TGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGAA1369040.10145682482009337RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)