FastQCFastQC Report
Fri 29 Sept 2023
SRR2163093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218920358
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA43971352.008554636111092No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA42098471.9230038898438124No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG34498441.5758443077276534No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA32783861.4975245015815295No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA24841201.1347140223477983RNA PCR Primer, Index 1 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA23365641.067312341961363No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA20871420.9533795847346458No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA20037570.915290390672575No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC19121770.873457826156122No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA16676040.7617400296778247No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG16587450.7576933525752777Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG15810110.7221854625324521No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT15281330.6980314731624914No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA15142840.6917054283275016RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA13911830.6354744769785183No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA13631830.6226844375980786No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA13584220.6205096741162829RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT13323520.608601233878852RNA PCR Primer, Index 1 (100% over 25bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC13291780.607151391557655No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA13231970.6044193477885689No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG12899330.5892247810046062Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA12749040.5823597273671551RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT12615560.5762625328796511RNA PCR Primer, Index 1 (100% over 25bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG11907000.5438964246532065No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG11670510.5330938660350628No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG11599500.5298502206907592No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG11595140.5296510615061208Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC11294660.5159255221024259No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC10432420.47653950940460277No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG10282090.4696726286186687Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC10267640.4690125712292138RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA9865190.4506291735554352No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA9850080.44993896821601215RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC9656060.4410763844996088RNA PCR Primer, Index 1 (100% over 24bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC9425300.43053556490164335No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC9139250.41746916931316186RNA PCR Primer, Index 1 (100% over 26bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCA8598190.39275424535894465No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG8570550.39149168575724697No Hit
CCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT8555050.3907836657201154RNA PCR Primer, Index 1 (100% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA8238130.3763071682899404No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC7926720.3620823605632876RNA PCR Primer, Index 1 (100% over 24bp)
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC7819460.35718286190633763RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA7373600.33681655134147004No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA7138870.32609438725657486No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA7062610.32261092867388785No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG7048680.321974624214711No Hit
GAATACAAGCTTGGGCTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCC6845320.3126854013275458RNA PCR Primer, Index 1 (100% over 27bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC6766090.30906627697000205No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA6680700.3051657717460886No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC6493940.2966348154793352No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA6409230.29276537177963136RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA6362420.2906271512674943RNA PCR Primer, Index 1 (100% over 22bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA6312110.28832905526310165No Hit
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA6279990.2868618550313169RNA PCR Primer, Index 1 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA6183030.2824328471087189No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6117940.2794596197398873No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA6067310.27714690654763136RNA PCR Primer, Index 1 (100% over 22bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA5814080.26557968628938566No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA5699060.26032572082674926No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA5515030.2519194674439551No Hit
CCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTC5466910.24972140781900237RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC5384690.24596570411236032RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA5321210.24306601946996634No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG5205170.23776546172101543No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA5194810.23729223026393917No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG5112830.23354748944819465No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5087310.23238176871609173No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4999060.2283506223756495No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA4884040.2230966569130131RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT4880610.22293997893060272RNA PCR Primer, Index 1 (100% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4860210.22200813320431353Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGTGGAATTCTCGGGTGCCA4799050.21921442317392884No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC4754110.21716162185336824No Hit
CGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGGAA4746810.21682816725523535RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG4611080.21062819566556712No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4435900.20262619888461902RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4291210.1960169460347767Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4290670.19599227953025733Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA4284140.1956939975404206No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG4265770.1948548795996396No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG4214810.19252709243239957Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG4083800.18654272436371586Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA4033230.18423275189418428No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA4027550.18397329680960964RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4005740.18297704409929752RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA3944260.18016871688104952RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3903720.1783169018936101Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA3893520.1778509790304655RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGAA3815140.1742706815781838RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA3761720.17183052477924415RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC3683990.1682799184898099RNA PCR Primer, Index 1 (100% over 24bp)
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC3664670.16739740577255954RNA PCR Primer, Index 1 (100% over 24bp)
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3664010.16736725782259135Illumina Small RNA Adapter 2 (100% over 21bp)
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3647520.16661401586050759No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGTGGAATTCTCGGGTGCCA3623810.16553097359725677No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA3571270.16313101406494138RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3466900.1583635268858824No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG3459330.1580177390354898No Hit
CCCTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTCCA3316820.1515080657779666RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC3263190.14905831644949163RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGTGGAATTCTCGGGTGCC3233210.14768886866154313No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAG3127170.14284509803332224No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA3061870.13986227813495536No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG3043750.13903457987219262No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA3040580.1388897783549212No Hit
CGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGG3010430.13751256518592025Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC2982520.13623767233196282No Hit
GCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCA2958940.1351605683012815No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGTGGAATTCTCGGGTGCCAA2953750.13492349578562263No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA2932580.13395647745103725No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC2840920.1297695667024261No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAG2838220.12964623417982898No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC2813360.12851066139769424RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT2808520.1282895764312609No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGATGGAATTCTCGGGTGCCAAGG2738230.12507881975964977Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC2735690.12496279583098435RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2696150.12315665955561794Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGTGGAATTCTCGGGTGC2646320.12088048933302036No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA2644880.12081471198763524RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAAG2468640.1127642957718898No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA2410600.1101131033231729RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA2382070.10880988966773021RNA PCR Primer, Index 1 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCCAAG2366440.10809593139802923No Hit
CCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2361630.10787621679295811RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2354030.1075290585812033RNA PCR Primer, Index 1 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTTGGAATTCTCGGGTGCCAAGGAAC2320480.10599653779115416RNA PCR Primer, Index 1 (100% over 24bp)
TCCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2319610.10595679731165067RNA PCR Primer, Index 1 (100% over 24bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAA2315650.10577590961184159No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCTGGAATTCTCGGGTGC2236840.10217597031336849No Hit
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA2232540.10197955185145458RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2202010.1005849807718659No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)