FastQCFastQC Report
Fri 29 Sept 2023
SRR2163094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197370852
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA42411392.14881729344716No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA40185752.036052922343366No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG35159801.781407925421531No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA29928731.5163703098368346No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA22035221.116437395730551RNA PCR Primer, Index 1 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA19743441.0003219725676618No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC18494020.937018805593442No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG16677500.8449829258476322No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA16143960.8179505654664753No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA15906150.8059016738702632RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA15785720.799799962357157No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG15707360.7958297712572067Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA15526480.7866652974675308No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG12684070.6426516312550548Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA12630130.6399187049159619No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG12202370.618245798523482Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA12012770.6086395168421322No Hit
CCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT11855830.6006879881128546RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA11550270.5852064721289241No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC11537570.5845630133876101No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA11004900.5575747324635352RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG10886110.5515561132603309No Hit
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT10872310.5508569218721313RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC10766780.54551013439411RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT10719930.5431364302972153No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC10669400.5405762751634674No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA10372930.5255553135069813RNA PCR Primer, Index 1 (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC10345190.524149837484615No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG9309730.4716871769900451No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG9149540.4635709836222423No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC9120930.46212142814279383RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG9114670.4618042587159729Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA8581270.4347789915807832RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC8349610.4230416961467036RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC8347570.42293833741975234RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA7932180.4018921699745209No Hit
CCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT7894790.3999977666408412RNA PCR Primer, Index 1 (100% over 25bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCA7691250.3896852003253246No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC7647780.38748274745249617No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC7599190.3850208844414372RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA7529280.38147882140165257No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA7055560.3574773036902126RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA7046580.3570223226274567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6923790.3508010392537597No Hit
GAATACAAGCTTGGGCTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCC6852560.34719209703771253RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA6499290.32929330416023134RNA PCR Primer, Index 1 (100% over 23bp)
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC6397670.32414462090886653No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG6289560.31866711504087747No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6095740.30884702265965797No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA5898680.2988627723003395RNA PCR Primer, Index 1 (100% over 22bp)
CGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGGAA5852940.2965453075107565RNA PCR Primer, Index 1 (100% over 23bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA5647160.28611924925976406No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA5637870.285648561723795No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5611190.28429679170660926No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC5551590.2812770955662693No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA5495890.27845499699215975No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA5429050.27506847870322815No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC5189850.26294916130777RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTC5172540.2620721321099633RNA PCR Primer, Index 1 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA5158030.2613369678315013No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA5122350.2595292034307072No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5117060.2592611800652307No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA5063090.2565267337448592No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG5017810.2542325753348828No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA4969030.25176108577572537RNA PCR Primer, Index 1 (100% over 22bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC4916410.2490950386128951RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA4876750.24708562336246084No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG4793300.2428575421055587No Hit
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT4792480.24281599595060774RNA PCR Primer, Index 1 (100% over 25bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA4772340.24179558185217745No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4741930.24025482749600738Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA4715200.23890052417669047RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGTGGAATTCTCGGGTGCCA4674740.23685057609215773No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA4637250.23495110615421572No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG4525510.22928968255150461No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4474390.22669963445260902RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA4388180.22233171491806702RNA PCR Primer, Index 1 (100% over 23bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4328720.21931911202369433No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA4310340.21838787016028083No Hit
TGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGAA4271060.21639770800604335RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4257270.2156990232782701RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG4256050.21563721070626982Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4115630.2085226850011267Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4086780.20706096967144874Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3808410.19295706338644167No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG3698220.18737417214979646Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3608060.182806121746893Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC3338610.16915415656208446RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGTGGAATTCTCGGGTGCCA3336850.1690649843270677No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC3328880.1686611759673612No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA3326160.1685233643314262RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA3274010.1658811302086288RNA PCR Primer, Index 1 (100% over 22bp)
GCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCA3254330.16488402248980513No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA3228380.16356923868373432RNA PCR Primer, Index 1 (100% over 23bp)
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3207910.1625321047912384Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA3181430.1611904679825773No Hit
CCCTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTCCA3035880.15381602547877737RNA PCR Primer, Index 1 (100% over 28bp)
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG2963690.15015844386181199No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2954160.14967559647561332Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2846610.14422646359149324RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC2782950.1410010633181033RNA PCR Primer, Index 1 (100% over 24bp)
TTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAG2761280.1399031301744596No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGATGGAATTCTCGGGTGCCAAGG2728480.1382412839764202Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG2659880.1347655934524719No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA2652900.1344119444749623No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA2647700.1341484810533219RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC2647450.1341358145426661No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA2611740.13232653016059331No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG2580810.13075942946225919No Hit
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC2538350.12860814929248013RNA PCR Primer, Index 1 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA2525950.12797989036395305No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC2514280.12738861764654083RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2469120.12510053916167926No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTTGGAATTCTCGGGTGCCAAGGAAC2424010.12281499397894882RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGTGGAATTCTCGGGTGCC2369790.12006788114792147No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC2339250.11852054020621038No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGA2313210.11720119645630349RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2310690.11707351802889314RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2275040.11526727360937775RNA PCR Primer, Index 1 (100% over 25bp)
CCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2238340.11340782984510803RNA PCR Primer, Index 1 (100% over 25bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAAG2198470.11138777472572292No Hit
TGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAGG2120110.10741758362577267Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA2097860.10629026417740751RNA PCR Primer, Index 1 (100% over 22bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAA2089180.10585048292743855No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAG2080140.10539246190212524No Hit
CGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGG2050350.10388312049238152Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCTGGAATTCTCGGGTGC2031220.1029138790970006No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGTGGAATTCTCGGGTGCCAA2026390.10266916211113077No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGTGGAATTCTCGGGTGC1993750.10101542247991106No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA1976750.10015409975531746RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)