FastQCFastQC Report
Fri 29 Sept 2023
SRR2163096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241004756
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA44712781.8552654620641595No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA43961021.8240727166396666No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA42854451.7781578551088844No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG37948681.5746029509890669No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA24771341.0278361477646525No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA23436370.9724442948337501No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA21914780.9093090262500878No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA21723680.9013797221495496RNA PCR Primer, Index 1 (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG20269090.8410244816911414No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC20244450.8400020952283614No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA18011980.7473703133061822RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA17860230.7410737570672672No Hit
CCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT16907170.701528479379884RNA PCR Primer, Index 1 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG16859060.6995322532141233Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG16104020.6682034108903644No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG15945540.6616276070502111Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA15618460.6480560906441198No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA15588210.6468009286920462No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG15323910.6358343401322752Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA15226770.6318037142802277No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC13550340.562243676220232No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC13467700.5588146982460379No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA13432690.5573620298181999RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA13209760.5481120048933805No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA13105800.5437983970739565RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA12752710.5291476488538674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG12711650.5274439480356147Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT12373990.5134334361434759RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC12338220.511949233068247RNA PCR Primer, Index 1 (100% over 24bp)
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC11797630.48951855539315586RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG11788180.4891264469486237No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA11709490.4858613661549484RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA11385140.4724031255217221No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC10881670.45151266641393584No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT10821740.4490259934953317No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC10629240.44103859925486283RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC10367700.4301865312566695RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA10209310.4236144617826546No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC9803720.4067853333151649No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC9368550.38872884317685413RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG9330210.38713800320189534No Hit
CCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT8914750.3698993392478943RNA PCR Primer, Index 1 (100% over 25bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA8865840.36786991871645885RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCC8567040.35547182313696746RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG8513140.3532353527496362No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG8360620.3469068469337593No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG8159600.33856593269885515No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA8019960.33277185617034044No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCA7972880.3308183677503858No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG7882760.3270790224571336Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA7804430.3238288791280119RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA7679950.31866383582903235RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC7610020.31576223333949477RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA7502320.31129344186054153No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG7114750.29521201647987394No Hit
CCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTC7048750.2924734813117132RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA6991310.2900901258562715No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC6869620.2850408479075824No Hit
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA6757890.280404839811543RNA PCR Primer, Index 1 (100% over 23bp)
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG6591510.2735012416103523No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC6337770.262972818677487No Hit
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT6232480.25860402522512876RNA PCR Primer, Index 1 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA6076080.25211452673572965No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA6050020.2510332202738771No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA6016990.2496627079010839No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA5850130.2427391930804884No Hit
CGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGGAA5834650.24209688210468347RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG5726250.23759904555576492Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA5674680.23545925375846108No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5644380.23420201715853278RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA5641150.23406799490712127No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA5483580.22752994965792292No Hit
TGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGAA5466860.2268361874153222RNA PCR Primer, Index 1 (100% over 23bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5424920.22509597279482735RNA PCR Primer, Index 1 (100% over 22bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC5401960.22414329449996412RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG5300900.21995001625611074No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC5244830.2176235061518869No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGTGGAATTCTCGGGTGCCA5228260.21693596785285016No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA5132010.21294227073261573RNA PCR Primer, Index 1 (100% over 23bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA5130560.2128821059448304No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA4862390.20175493964110813No Hit
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4833000.20053546163213476Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC4737120.19655711690602487No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG4709560.1954135710085323No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4569600.18960621673374778No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4500080.18672162635661846Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA4469490.18545235679913305RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGTGGAATTCTCGGGTGCCA4457590.18495859060972225No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA4314070.1790035214076854No Hit
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4305030.1786284250755616Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3934540.16325569940204832Illumina Small RNA Adapter 2 (100% over 21bp)
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3916090.1624901543436761No Hit
CCCTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTCCA3768340.15635956993313443RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA3688240.15303598406995753RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC3514370.14582160362013769RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA3495550.14504070616764092RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTTTCCGGCTCGAAGGATGGAATTCTCGGGTGCCAAGG3452590.14325816873091085Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG3437320.14262457127609549Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG3387370.1405519980692829Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGTGGAATTCTCGGGTGCC3344730.1387827383788227No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC3310920.1373798614994967RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT3282180.13618735391263398No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG3263730.13542180885426178No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG3225840.13384964070999494No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT3210980.13323305536758784RNA PCR Primer, Index 1 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTTGGAATTCTCGGGTGCCAAGGAAC3136120.13012689259958007RNA PCR Primer, Index 1 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA3065570.1271995644766446RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCA3044130.1263099554765633No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAG2967070.12311250820295015No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA2954680.12259841046456361No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGA2936390.12183950427932634RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA2935050.12178390371682125RNA PCR Primer, Index 1 (100% over 23bp)
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC2911470.12080549978856019RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG2870900.1191221305192832No Hit
TCCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2852240.11834787193992138RNA PCR Primer, Index 1 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA2837790.11774829870992255RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA2825440.11723586068981975No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA2780080.11535374015606563RNA PCR Primer, Index 1 (100% over 22bp)
TGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAGG2759370.11449442101466246Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTTGGAATTCTCGGGTGCCAAGGAA2714740.1126425903395865RNA PCR Primer, Index 1 (100% over 23bp)
GCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG2697840.11194135936470898No Hit
CCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2653000.1100808151686434RNA PCR Primer, Index 1 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA2616170.10855262955889551No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2599880.10787670928784493No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAA2575460.10686345127562545No Hit
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG2572060.10672237522150807Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCTGGAATTCTCGGGTGC2559500.10620122368041568No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCAA2469660.10247349641514958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)