FastQCFastQC Report
Fri 29 Sept 2023
SRR2163097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208062238
Total Bases10.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA42448252.040170787742848No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA39495261.8982425825872353No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA39394491.8933993202553172No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG32262601.5506225593901377No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA30030851.4433589818446537RNA PCR Primer, Index 1 (100% over 22bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA21589601.037651051316674No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC20273860.9744132426375227No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA20140410.9679992964412888No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA19927850.9577831225673926RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG19723030.9479389527666237Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAAG17510530.8416005791497829No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA16949820.8146514313664165No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG16436300.789970354928125No Hit
CCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT15260820.7334737983545097RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA14841740.7133317483588733No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC14276370.6861586291309623No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA13812840.6638801991546395No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA13484100.6480801191804925No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA13381130.6431311192567293No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG12553430.6033497534521377Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA12151710.5840420691812418RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT11477100.5516185978928094RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG11247490.5405829576821143No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA10760070.5171563135834384RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC10572610.50814650950741RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC10476330.5035190479879391No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA10410400.5003502846105116RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG10253010.4927857211648372Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG10153590.48800734326427847Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA10084690.48469583413786027No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA9893590.47551108240987006No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC9857510.47377698590361217RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC9840530.4729608839447358RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA9591180.46097648915994066No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC9306230.4472810678889266RNA PCR Primer, Index 1 (100% over 24bp)
CCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT8925510.4289826969947329RNA PCR Primer, Index 1 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC8733640.41976093710959694RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA8652610.41586642935177887No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC8104940.38954401711280257No Hit
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA7928390.3810585753672418RNA PCR Primer, Index 1 (100% over 23bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCA7909120.3801324101877631No Hit
CGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGGAA7625310.3664917802143414RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC7201840.3461387356604325RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA7156020.3439365099975518RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCC7129880.34268015515626626RNA PCR Primer, Index 1 (100% over 27bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA7035550.3381464155932034RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG6994390.3361681613748671No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC6906040.33192183581145557No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6859340.3296773151118369No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG6803740.3270050377906634Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA6787430.32622113773475797No Hit
CCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTC6685790.3213360609915193RNA PCR Primer, Index 1 (100% over 26bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA6641680.31921602227502716No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG6546180.31462604953811946No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG6497420.3122825200025004No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC6414270.30828611965617714No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA6207380.29834246039399037No Hit
CCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT6202940.2981290627086305RNA PCR Primer, Index 1 (100% over 25bp)
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG6151870.2956745087015742No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6094800.2929315794440316No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG5929620.2849926087981424No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGTGGAATTCTCGGGTGCCA5832460.2803228522419335No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA5472470.26302081783817016No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA5464960.26265986814964476RNA PCR Primer, Index 1 (100% over 22bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA5358030.2575205405605605No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA5042050.24233373861911453No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG4729670.22731996182796033Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4671460.2245222412728253No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4512480.21688125838577205RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4509820.2167534120247231Illumina Small RNA Adapter 2 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA4487750.21569267172835083No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA4486370.21562634542073894RNA PCR Primer, Index 1 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA4436740.21324100147379937No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA4383180.21066677173779128No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC4374170.2102337282366443RNA PCR Primer, Index 1 (100% over 24bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4358010.20945703756200104RNA PCR Primer, Index 1 (100% over 22bp)
TGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGAA4313750.20732978946424674RNA PCR Primer, Index 1 (100% over 23bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4289280.20615369906767994No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC4249900.20426099617365454No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA4208800.20228562570782307RNA PCR Primer, Index 1 (100% over 22bp)
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4186480.20121286977601385Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGTGGAATTCTCGGGTGCCA4185000.2011417372142272No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG4085730.1963705686949306No Hit
CCCTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTCCA4019440.19318450280247393RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3939620.18934815072017055No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG3910570.18795193388239917No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGATGGAATTCTCGGGTGCCAAGG3690050.17735318217619095Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3601070.17307657721147843No Hit
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3486510.1675705324288591Illumina Small RNA Adapter 2 (100% over 21bp)
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3481240.16731724283384858No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG3443240.16549086624743506Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAGG3385040.1626936263177175Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3301550.15868088470719996Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCAAG3276820.15749229804977874No Hit
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC3245050.15596535109845353RNA PCR Primer, Index 1 (100% over 24bp)
TGCGAGAGGTCCCGGGTTCATATCCCGGACGTGGAATTCTCGGGTGCCAA3195800.1535982709173781No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT3193950.1535093552151448RNA PCR Primer, Index 1 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA3143760.1510970962448265No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA3017300.14501910721540925RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC3004430.14440054230311605RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC2896370.13920690404185695RNA PCR Primer, Index 1 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA2884660.13864409167799108RNA PCR Primer, Index 1 (100% over 23bp)
TCCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2856620.13729641800738487RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA2795040.13433672668656002RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA2739600.13167213937206615RNA PCR Primer, Index 1 (100% over 22bp)
GCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCA2715410.1305095064871887No Hit
CGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAGG2670640.12835774649314308Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA2658650.12778147661758785RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2626630.1262425140308257No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2620420.12594404564657236Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACT2603730.12514188182480282RNA PCR Primer, Index 1 (100% over 25bp)
GTCGCTGGTTCGTTTCCGGCTCGAAGGATGGAATTCTCGGGTGCCAAGGA2589090.12443824621361614RNA PCR Primer, Index 1 (100% over 22bp)
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCAAG2580310.12401625709707112No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGA2567610.12340586281687502RNA PCR Primer, Index 1 (100% over 22bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAA2566240.12334001713468062No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC2525730.12139300356848032No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA2517780.12101090636158592RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2499160.12011598183424327No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGTGGAATTCTCGGGTGCC2484560.1194142687247265No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA2480590.11922346043398803RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG2462040.11833190028456775No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGTGGAATTCTCGGGTGCCAA2394690.11509488809785849No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG2366970.11376259444061156No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTTGGAATTCTCGGGTGCCAAGGAAC2285870.10986472230487111RNA PCR Primer, Index 1 (100% over 24bp)
AACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCCAAGGAA2251030.10819022335038038RNA PCR Primer, Index 1 (100% over 23bp)
TCTTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGG2229720.10716601058573638Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAAG2212390.1063330867372483No Hit
CGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTC2160200.10382470268343456RNA PCR Primer, Index 1 (100% over 26bp)
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCTGGAATTCTCGGGTGC2145430.10311481894182067No Hit
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG2132180.10247799026366332Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA2125150.1021401105951768No Hit
TCCCTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAACTCC2117410.10176810652204943RNA PCR Primer, Index 1 (100% over 27bp)
TCTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGGA2103030.10107696717171713RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA2082180.10007486317627709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)