FastQCFastQC Report
Thu 28 Sept 2023
SRR2163103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152204602
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG29074701.9102379046331335No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG25465161.6730873879884394No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA25188261.6548947711843824No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA24190831.5893625870786747No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA23216741.5253638651477832No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG14657560.9630168738261936No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA12840470.8436321787431894No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA12046990.7914997208822898No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC9928300.6522995934117681No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA9517750.6253260331773673No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA9279070.6096445099603492No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA9155750.601542258229485No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA8607190.5655012980487936RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA8443450.5547434104522018RNA PCR Primer, Index 1 (100% over 22bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG8215480.5397655453282549No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC7866250.5168207726071252RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA7575680.4977300226441248No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT7261570.47709267029915425No Hit
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT7023290.46143742749644323RNA PCR Primer, Index 1 (100% over 25bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC6722660.4416857251136204No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG6716680.44129283291973Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC6579450.432276679781338No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG6260730.4113364456614787No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC6144300.4036868740670535RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA6077150.39927504951525705RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCCGGTGCCAA5927880.38946785590622285No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5721680.3759203023309374RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA5637180.3703685648085726RNA PCR Primer, Index 1 (100% over 23bp)
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA5597690.3677740309061089RNA PCR Primer, Index 1 (100% over 22bp)
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA5542690.3641604739388892No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG5449120.358012828022112Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG5376510.3532422758150243No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG5189870.3409798344993537No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA5003710.32874892968085156RNA PCR Primer, Index 1 (100% over 23bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC4907710.322441630247159No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA4904220.32221233363233No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG4729360.31072385051800205No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA4725050.3104406790538436RNA PCR Primer, Index 1 (100% over 22bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4643390.3050755324730589RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4556860.2993904218480858No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC4508380.2962052356340711RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC4452030.292502982268565No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4383170.287978808945606Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA4365910.2868448090682567No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA4183460.27485765509245247No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG4144940.2723268511946833Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAG4100390.2693998700512354No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4073040.2676029467229907No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG4068020.2672731275234372No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA4004820.2631208220629229RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA3775880.24807922693428153RNA PCR Primer, Index 1 (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC3744790.24603658173226586No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC3700140.24310303048524118No Hit
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3624940.23816231259551532Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG3481370.2287296148903566No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3345880.2198277815542003No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3334560.21908404582931074Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA3210190.2109128080108905No Hit
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3192100.20972427627385407No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA3137880.20616196611453314No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3105150.20401157121385857No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA3075490.20206287849299062No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC3058360.2009374197502911No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA3033210.1992850387007352No Hit
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3022150.19855838524514524Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG2908380.19108357840586188No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA2888630.18978598294945118RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA2803970.18422373326136354No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA2784930.18297278554034785RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2666040.17516158939793425No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA2625890.17252369281186386No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA2538700.16679521950328413No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2527600.16606593800626346Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA2384830.1566858011297188No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2224470.14614998303402155RNA PCR Primer, Index 1 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG2140640.14064226520562106Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAA2135070.14027631043639535No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA2116550.13905952725397883No Hit
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC2113990.13889133260241368No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG2065500.13570548937804128Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA2048210.13456951846961893RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC2034520.13367007128996009No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA1988130.13062220024069968RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC1986220.13049671126238352No Hit
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC1974850.12974969048570556RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC1970470.12946191994904332RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1939940.12745606732705755No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1934180.127077629361036No Hit
TAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC1827560.12007258492749123No Hit
TGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA1822650.11974999284187215RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1797300.11808447158516272RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG1756090.11537693190117866Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1748820.11489928537114796No Hit
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC1748520.11487957506041768RNA PCR Primer, Index 1 (100% over 26bp)
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC1714420.11263916974074148No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1686850.11082779218462789No Hit
TAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA1684920.11070098918559639No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA1684680.11068522093701215RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAA1634610.10739557007612688No Hit
AAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAGGA1611300.1058640789323834RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1601550.10522349383364901No Hit
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG1581150.10388319270398932Illumina Small RNA Adapter 2 (100% over 21bp)
AATCCAGGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTG1578930.10373733640458518No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG1563240.10270648715339106No Hit

[FAIL]Adapter Content

Adapter graph