FastQCFastQC Report
Thu 28 Sept 2023
SRR2163104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137804898
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA27842842.020453583587428No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA25483291.8492296260761356No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG22749621.650857141521922No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG21711161.5754998780957696No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA18738741.359802174810942No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG18560711.3468831855308945No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA14034441.0184282419337518No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA14018521.0172729854638403No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA13182880.9566336314112726No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA10717120.7777024006795462No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC8765380.6360717309191725RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA8645490.6273717498778599RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA8527340.6187980343049926RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC8072530.5857941275788324No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG7495520.5439226115170449Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG7441640.5400127359769171No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA7290390.5290370738491458RNA PCR Primer, Index 1 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA7226430.5243957293883705No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6639930.4818355585590289No Hit
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT6508210.47227711746501205RNA PCR Primer, Index 1 (100% over 25bp)
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA6461260.4688701268078294RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6395330.4640858266155387No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG6334590.45967814583774813No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC6271240.45508106685728983No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG6205440.4503062002919519Illumina Small RNA Adapter 2 (100% over 21bp)
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG6141750.44568444874869395No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG6052580.4392137063226882No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC6050560.43906712227311395No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA5800990.42095673551458235RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC5652970.41021546273340737RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC5206090.37778700725136777No Hit
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5029140.36494638964139RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG4918320.3569045854959379No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA4855330.3523336304055027RNA PCR Primer, Index 1 (100% over 22bp)
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA4732250.3434021626720409No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA4590120.33308830575818865No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA4526190.3284491382882487RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4349740.31564480385885846Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4268890.30977781355783157Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC4261310.30922776054012246RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA4258570.30902892871050197No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4255550.3088097782997525No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA3977640.28864286086551144No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCCGGTGCCAA3940850.2859731444378704No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA3905750.2834260651606157No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA3900850.2830704899908565No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA3860790.2801634815621721No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG3845610.27906192419953024No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA3793600.2752877477548004RNA PCR Primer, Index 1 (100% over 23bp)
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3698230.26836709388950747Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA3685060.2674113949128281No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG3663360.26583670487532307Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC3613230.2621989531896029No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA3587770.26035141363407854No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA3361410.24392529211842673No Hit
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3336040.24208428353540815Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3260600.2366098772483399No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA3258260.23644007196318959RNA PCR Primer, Index 1 (100% over 22bp)
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3185440.2311557895424007No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3118530.2263003743161582No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT3026200.21960032218883832No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC2822270.2048018641543496No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAG2794980.20282152815787433No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG2787720.20229469637574132No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA2771890.20114597087833555RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2681260.19456928156501374Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC2656130.19274568890867724RNA PCR Primer, Index 1 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA2655620.19270868006447783No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA2629190.19079075113861338RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2601430.1887763089523857No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC2562660.18596291112961746No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA2473820.17951611560279954RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2459430.1784718856654863No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA2454440.1781097795232213RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2433690.17660402752883284No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA2431400.17643785056174127No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA2319010.1682821172292439No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA2304500.1672291793285896No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2242180.1627068437001419RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA2233080.16204648981344624RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC2159850.15673245518457551No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC2059810.1494729164125937No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC2052160.14891778374960227No Hit
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC2042980.14825162455401258RNA PCR Primer, Index 1 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA1972930.14316835095367944No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG1879120.13636090061182005Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA1847320.13405329032644397RNA PCR Primer, Index 1 (100% over 23bp)
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG1835510.13319628160096314Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA1823420.13231895429435317No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1743070.12648824717391394Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1705090.12373217677647425RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1696900.12313785827844813No Hit
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC1694210.12294265476688646No Hit
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC1661070.1205378055575354RNA PCR Primer, Index 1 (100% over 26bp)
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG1653210.11996743395869718Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA1624820.11790727496492903No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1620090.11756403607656965No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAA1586610.115134514304419No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1584030.1149472930925866No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC1578230.11452640819776957RNA PCR Primer, Index 1 (100% over 24bp)
CCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG1567320.11373470919734652No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGA1537840.11159545286989726RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG1536910.1115279661540042Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAA1520050.11030449730458783No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA1498720.10875665682071765No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA1497580.10867393116897775RNA PCR Primer, Index 1 (100% over 22bp)
TAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA1476810.10716672784736576No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG1474720.1070150641525093Illumina Small RNA Adapter 2 (100% over 21bp)
AATCCAGGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTG1470280.10669286950889074No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG1449230.10516534760614966Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA1401580.10170756049614435RNA PCR Primer, Index 1 (100% over 22bp)
AAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAGGA1390220.10088320663319238RNA PCR Primer, Index 1 (100% over 22bp)
TAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC1381390.10024244566401407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)