FastQCFastQC Report
Thu 28 Sept 2023
SRR2163105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156238696
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG32219382.062189510337439No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA31462432.0137412053157435No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA30776721.9698525901675472No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG28675211.8353462192234375No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA26796211.715081518601512No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA16983051.0869938392214946No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA15318450.9804517313687768No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG14977390.9586223121063429No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA14885310.9527287657341944No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA10915940.69867070575141No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC9990570.6394427408687539RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT9540220.6106182555440683No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC9272900.5935085377312673No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG8464660.541777435213617No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA8315600.5322369049982343RNA PCR Primer, Index 1 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA8189540.5241684812832795No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG7974900.5104305274027633No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA7679480.49152227947422195No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC7590950.4858559495401831RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA7461850.4775929517486501RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC7329900.46914754076032483No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA6936830.44398924066800965RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC6457830.4133310226808345No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA6401750.4097416430050082RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG6271690.4014172007682399No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA6015220.38500193319585824RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT5983210.3829531449750451RNA PCR Primer, Index 1 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC5784630.3702431054596103No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG5709090.36540819567516103Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5635990.360729457189018RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG5612810.3592458298551084No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG5295810.3389563620013828Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA5114480.32735040236126905RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA5071310.3245873224645961No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG4844250.31005443107384867No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA4800330.3072433477043357RNA PCR Primer, Index 1 (100% over 23bp)
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4745030.30370389164026307No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4620210.2957148336670705No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC4419580.2828735846592063RNA PCR Primer, Index 1 (100% over 24bp)
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG4419240.2828518230848522Illumina Small RNA Adapter 2 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA4409180.28220793650249104No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA4388740.28089968185602365No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA4364790.2793667709566649RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG4136890.26478011567633664No Hit
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG4074790.260805428125181No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA3972270.2542436734110991No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA3835840.24551152167834275No Hit
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3707770.23731444865617674Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3705320.2371576373115659Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3664090.2345187263979725Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA3633810.23258066618784376No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3586900.22957820897327508No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC3577520.22897784553962225No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3422460.21905328754151915No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3397150.21743333034474377No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC3299950.21121208026467397No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA3262010.20878374458527227No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA3214610.20574992510178144No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA3130000.20033449331911984No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA3101820.19853084283294326RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCCGGTGCCAA3076540.19691280577508147No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2980680.19077732189981925Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG2973180.1902972871714188No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA2925280.18723146537270127RNA PCR Primer, Index 1 (100% over 22bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC2846980.1822199028082006No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA2844930.18208869331577116No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2820660.1805353009346673RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA2808950.17978580671205807RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAA2706340.17321829158123542No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2649880.16960459014583687Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2606510.1668287093230732No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC2548980.1631465229330895No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA2471080.1581605622207702No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC2443350.15638571381829763No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA2425780.15526115246123148RNA PCR Primer, Index 1 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA2397620.15345878206766395No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA2357490.1508902762475693No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG2345840.15014462230278727Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA2331140.1492037542351224RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA2254640.14430740000543782No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC2240910.14342861642931273RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA2177770.1393873640624855No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG2146900.13741154112038928Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2075480.13284033041340795RNA PCR Primer, Index 1 (100% over 23bp)
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG2041270.13065073200559738Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC2034570.13022190098155967No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC2007420.12848417526475003No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAG2002550.12817247271444202No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2002390.12816223197356946No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1987230.12719192177589603No Hit
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC1952120.1249447192006774RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1901320.12169328397364505No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG1857830.11890972259522697No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG1823290.1166990026593668Illumina Small RNA Adapter 2 (100% over 21bp)
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC1777310.11375606975111978No Hit
TAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA1761710.11275759751604687No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1760170.11265903038514863RNA PCR Primer, Index 1 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA1736850.11116644240297552RNA PCR Primer, Index 1 (100% over 23bp)
AATCCAGGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTG1671360.1069747791545828No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG1671340.10697349906197375Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA1650420.10563452219288876RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA1641930.10509112288033945No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA1610600.10308585780823465RNA PCR Primer, Index 1 (100% over 23bp)
AAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAGGA1595020.1020886656657708RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)