FastQCFastQC Report
Thu 28 Sept 2023
SRR2163106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152797784
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA30206261.9768781463479865No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG27992501.8319964640324888No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG26546571.737366164943858No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA24038411.573217187495337No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA23840841.5602870261521593No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG18235491.1934394284147471No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA14569110.953489613435755No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA13929250.911613351670074No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA13841230.9058527969227617No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA13231440.865944495634832No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT11745720.7687101011883785No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA9287680.6078412760226941No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC8782460.5747766603735562No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC8607930.5633543743016587RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA8335950.5455543779352192RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG7789530.5097933881030631Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA7520340.4921759860077552RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA7083260.4635708591166479RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6849560.44827613468530414No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC6729280.4404042927742984No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG6598050.4318158174335827Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC6554410.4289597550707934No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG6485000.42441714992411145No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA6474230.4237122967699584RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT6229630.4077042112076704RNA PCR Primer, Index 1 (100% over 25bp)
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG6112050.40000907343001785No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC5994570.3923204802498968RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC5992370.3921764991042017No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG5823280.3811102391380231No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5750850.3763699871458869RNA PCR Primer, Index 1 (100% over 22bp)
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5673700.37132083015025924No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG5232930.342474207610236No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG5194640.33996828121538725No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA4800510.31417405896410117RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC4691210.3070208138620649RNA PCR Primer, Index 1 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA4623580.3025947025514454No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA4603350.30127073047080316No Hit
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4545900.2975108591889003RNA PCR Primer, Index 1 (100% over 22bp)
TCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA4523000.2960121463541644No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA4515180.29550035882719344No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA4459760.291873342875182RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCCGGTGCCAA4378410.28654931278322726No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC4027870.2636078805959647No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4002240.26193050024861614No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA3993120.26133363295373446RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3947890.2583735114901928No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3943720.2581006017731252Illumina Small RNA Adapter 2 (100% over 21bp)
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3875480.25363456841756293Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG3862890.25281060358833474No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA3843270.2515265535526353No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA3823740.25024839365471424No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA3719020.24339489111962512No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA3666090.23993083564615045No Hit
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3471620.22720355682645238No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA3382720.22138540962086203No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3361830.22001824319651128No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA3287940.21518244008041373No Hit
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3279490.21462942158899373Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA3087470.20206248540881983No Hit
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3073960.20117831028230096No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAG3070560.20095579396622662No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA3057390.20009387047131522RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA3048820.19953299846285727RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG3032380.19845706662866264Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG3020550.19768284074067463No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC2991930.19580977692713136No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC2975530.19473646293194932No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG2939550.19238171674008048No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC2909840.19043731681344278RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2889890.18913166960588906No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG2801050.18331744915881765Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA2783350.18215905539572486No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC2744600.17962302385223075No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2654520.1737276503957675Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA2579600.1688244379250945No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA2522030.16505671312615372RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA2311290.1512646282880647No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2292310.15002246367656746No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA2285120.1495519071140456RNA PCR Primer, Index 1 (100% over 22bp)
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA2206130.14438232952383656RNA PCR Primer, Index 1 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC2180600.14271149377401968No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2071360.1355621754305023No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2068000.13534227695344064RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG2022310.13235205034125364Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA2019660.13217861850666632No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC2010340.1315686620167214No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA1998460.13079116382996758No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA1957440.1281065699225062RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC1947850.12747894301922597RNA PCR Primer, Index 1 (100% over 24bp)
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC1935890.1266962091544469RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC1851360.12116406086098735RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1839090.12036103874386032RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG1770710.1158858429517538Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAA1756300.11494276644745058No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA1746530.11430335926861349RNA PCR Primer, Index 1 (100% over 22bp)
TGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA1718960.112499013729152RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1717600.11241000720272226No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG1713130.11211746369305985Illumina Small RNA Adapter 2 (100% over 21bp)
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC1711640.11201994918983904No Hit
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG1709800.11189952859525763Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA1632060.10681175847419358No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA1622640.10619525738671708RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG1609230.10531762685772983No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAA1608650.10527966819204655No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG1588190.10394064353708166Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA1582220.10354993106444527RNA PCR Primer, Index 1 (100% over 23bp)
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC1556670.10187778639512207RNA PCR Primer, Index 1 (100% over 26bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA1542960.10098052207354001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)