FastQCFastQC Report
Thu 28 Sept 2023
SRR2163107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162599027
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA33568482.0644945187771633No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG28230731.7362176466160526No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA24347461.4973927242504348No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA23806861.4641452928251533No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA22501571.383868674687703No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG21968071.3510579002419245No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG16677441.0256789544011233No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA15837850.9740433440601094No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA13793310.8483021242187383No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAA13513220.8310763138822473No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT13207420.8122693132720898No Hit
ATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGAAC10999470.6764782177940093RNA PCR Primer, Index 1 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA9686090.5957040567038572No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA9445040.580879244744804RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA8983040.5524657905855734RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA8889870.5467357439967953RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC8653800.5322172069332247No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG8565120.5267632997582452Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG8404520.5168862418838459Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA8082650.4970909204764184RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT7619110.4685827548033236RNA PCR Primer, Index 1 (100% over 25bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCTGGAATTCTCGGGTGCC7380380.45390062512489693No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAAG7175130.4412775483582691No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA6943000.4270013251678314No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC6449240.3966345997876113No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGCTGGAATTCTCGGGTGCC6346320.3903049186143039No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC6203370.3815133530903601RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6078420.3738288052609319No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG5900150.3628650250164166No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG5872630.3611725179634685No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA5771100.3549283231565709RNA PCR Primer, Index 1 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA5394210.33174921766290766No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA5377670.33073199140361403No Hit
ACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA5308190.32645890310278425RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG5268500.3240179290863776No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC5218470.3209410349054549RNA PCR Primer, Index 1 (100% over 24bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC5208900.32035247049787086No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGTGGAATTCTCGGGTGCCA4974120.30591326970240723No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4826230.296817889322302No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC4710400.28969423045809495RNA PCR Primer, Index 1 (100% over 24bp)
ATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGGA4594230.2825496612596581RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG4529100.2785441022350029No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAA4477420.2753657314320829No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA4280670.2632654130211985No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA4279490.26319284186122466No Hit
TCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCA4254950.2616836077377019No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG4194040.2579375828614276Illumina Small RNA Adapter 2 (100% over 21bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG4180850.25712638489527984No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA4106560.2525574768660823RNA PCR Primer, Index 1 (100% over 23bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGCC4091940.25165833249420366No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC4087600.2513914182278594No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA4085500.2512622661634992RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4029030.24778930565187207Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG4022850.2474092295767551No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA3983330.24497871072746333No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGTGGAATTCTCGGGTGCCAAGGA3929230.241651507545614RNA PCR Primer, Index 1 (100% over 22bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3877530.23847190672303345No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA3744860.2303125713046241No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA3646330.22425287944681244No Hit
CACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3544010.21796009886332224Illumina Small RNA Adapter 2 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC3524200.21674176438952492No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA3474630.21369316066079536RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA3405970.20947050316604907No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG3338470.20531918681161604No Hit
CCCATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG3291340.20242064548147634Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC3264590.2007754941854603RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG3196530.1965897372805312Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG3195880.1965497616415626No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCCGGTGCCAA3188350.19608665923935695No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA3147070.19354789865993477No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA3137550.19296240930150216No Hit
CCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAG3096970.19046669941020003No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCGTGGAATTCTCGGGTGCCA3088060.18991872565141488No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG3038540.18687319697183674Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA3035440.18668254392444797No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC3004080.18475387309666988RNA PCR Primer, Index 1 (100% over 24bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCA2838190.17455147502204918No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCTGGAATTCTCGGGTGCCAA2719520.16725315336604074No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAA2693920.16567872820050764RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA2527940.15547079503741434No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA2512040.154492929407259RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2484440.1527955022756686No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA2373260.14595782298254467RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA2340110.14391906539514532No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG2304010.1416988799078115Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG2279400.14018534071547673No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA2210940.1359749834173362No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGCTGGAATTCTCGGGTGCC2201320.1353833439606007No Hit
CCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC2147200.13205491075909082RNA PCR Primer, Index 1 (100% over 24bp)
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA2132190.131131781003831RNA PCR Primer, Index 1 (100% over 22bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGGA2100020.1291532943797997RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGTGGAATTCTCGGGTGCCA1944750.11960403674494313No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGAAC1944740.11960342173511285RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA1922980.11826516034440969No Hit
CCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACTC1905260.11717536292514222RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCCGGTGCCAAG1899030.1167922118008738No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCA1880930.11567904400805547No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG1846420.1135566450837372Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1844230.11342195793090447No Hit
CCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA1842810.11333462653500381RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1790660.11012735027006035No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1731040.10646066166189298RNA PCR Primer, Index 1 (100% over 22bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG1719080.10572510990487047Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA1713810.10540099972430955RNA PCR Primer, Index 1 (100% over 22bp)
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCC1701280.10463039240696072No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGTGGAATTCTCGGGTGCCA1701170.10462362729882757No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG1688510.10384502485368502Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC1684850.10361993125580021No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1667930.10257933462295564Illumina Small RNA Adapter 2 (100% over 21bp)
CATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCAAGG1663500.10230688526813878Illumina Small RNA Adapter 2 (100% over 21bp)
TAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA1654260.10173861618495417No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC1651970.10159777893381859No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)