FastQCFastQC Report
Thu 28 Sept 2023
SRR2163113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68962345
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG24586963.565273193653725No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG14744152.1380000926592624No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG11993671.7391621471108616Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA11446151.659768095182958No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC6475690.939018242491609No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA4931090.7150409400956421No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG3935760.5707114512999812No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA3616030.5243484687186899No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC3435160.4981211123258642RNA PCR Primer, Index 1 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGCC3387650.4912318454368104No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA3357320.48683379313739983No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGC3320710.48152509895073903No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA3104530.45017755704218004No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG2989920.4335583425998637No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA2513180.3644278627706178No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA2202890.31943374315360074RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG2126000.30828418030158344Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA2013140.29191872753166964RNA PCR Primer, Index 1 (100% over 23bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG1867450.27079270578748443No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGA1795370.26034062501789923RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG1683440.24411002845103366No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA1608590.23325627920570274RNA PCR Primer, Index 1 (100% over 23bp)
AAAGGACTTGGCGGTACTTTATATCCATCTAGATGGAATTCTCGGGTGCC1566350.22713119746725552No Hit
ATACGTACACCCTCTAACCTAGAGAAGGTTTGGAATTCTCGGGTGCCAAG1552770.2251620068894119No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA1391750.20181303289498057No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAAG1384160.20071243227010332No Hit
CCGCCGATGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1364090.19780214840432123No Hit
ATACGTACACCCTCTAACCTAGAGAAGGTTATGGAATTCTCGGGTGCCAA1362480.1975686876657109No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG1309940.18995003722683734Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTACACCCTCTAACCTAGAGAAGGTTTGGAATTCTCGGGTGCCAAGGA1205150.17475478828337407RNA PCR Primer, Index 1 (100% over 22bp)
AGAACTAGTACCGCAAGGGAAAGATGAAAGACTTGGAATTCTCGGGTGCC1150720.16686207523830576No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGG1130520.1639329405054309Illumina Small RNA Adapter 2 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCCTGGAATTCTCGGGTGCCAAGG1118920.16225086313407702Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA1106010.1603788270250961No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAA1102790.15991190554787543No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1045750.15164072509425253No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA1033300.14983539205344598RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA1006840.14599851556671978RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC996380.1444817457991024No Hit
AGGCATACGCGTATAACAACTCGGATAACCTGGAATTCTCGGGTGCCAAG982580.14248065375387103No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCTGGTGCCAAG957490.13884243640496854No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGG918860.13324082874502022No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG917200.13300011767291267Illumina Small RNA Adapter 2 (100% over 21bp)
ATATACGTACACCCTCTAACCTAGAGAAGGTTATGGAATTCTCGGGTGCC876690.12712589747346903No Hit
ACGTACACCCTCTAACCTAGAGAAGGTTATTAGTGGAATTCTCGGGTGCC875590.1269663901365303No Hit
AACGGACCAAGTTACCCTAGGGATAACAGCGCTGGAATTCTCGGGTGCCA869780.1261239013841539No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGC852850.12366893846199692No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG833580.12087465993217023No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA821180.1190765772248609No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG809620.11740030012030479No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA797520.11564571941397875RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGATGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG784120.11370263003672512No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAG775560.1124613729420019No Hit
AAAAGAATTACAGCTAGAAACCCCGAAACCTGGAATTCTCGGGTGCCAAG775530.11245702274190357No Hit
ACGTACACCCTCTAACCTAGAGAAGGTTATGGAATTCTCGGGTGCCAAGG766020.11107800931073326Illumina Small RNA Adapter 2 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTGGAATTCTCGGGTGCCA763650.11073434350296527No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT737030.10687426594904799No Hit
ATATACGTACACCCTCTAACCTAGAGAAGGTTTGGAATTCTCGGGTGCCA731460.10606657879745825No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC724670.10508198350853643No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAA722920.1048282218361339No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCC708420.10272562512194156No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCA706880.10250231485022733No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG702480.1018642855024724No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA699170.10138431342495675RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)