FastQCFastQC Report
Thu 28 Sept 2023
SRR2163114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37728610
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG13012523.448979434969907No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG7774732.0606987641474204No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG6310931.6727173357301Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA6072211.6094443977660455No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC3417910.9059199371511433No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA2595880.6880401901898852No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG2055810.5448941797749771No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA1897250.5028677176286113No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC1813250.4806034465621713RNA PCR Primer, Index 1 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGCC1773820.4701524917032459No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA1756810.46564397681229175No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGGAATTCTCGGGTGC1741900.46169206869799867No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGTGGAATTCTCGGGTGCCA1630760.4322343176703303No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG1568250.41566598928505455No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAA1316590.3489632933733843No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA1152250.30540483733697055RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG1111180.2945191990905575Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA1068950.28332610186275087RNA PCR Primer, Index 1 (100% over 23bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTTGGAATTCTCGGGTGCCAAG967550.2564499460754054No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGA943830.25016294000759637RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG890970.2361523522865009No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA842830.22339280455866253RNA PCR Primer, Index 1 (100% over 23bp)
AAAGGACTTGGCGGTACTTTATATCCATCTAGATGGAATTCTCGGGTGCC832440.2206389262684207No Hit
ATACGTACACCCTCTAACCTAGAGAAGGTTTGGAATTCTCGGGTGCCAAG801060.2123216307200292No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA728320.19304183217987622No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAAG723100.19165826676360462No Hit
ATACGTACACCCTCTAACCTAGAGAAGGTTATGGAATTCTCGGGTGCCAA706520.18726372373644298No Hit
CCGCCGATGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG698610.18516717154435322No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG682720.18095551360095163Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTACACCCTCTAACCTAGAGAAGGTTTGGAATTCTCGGGTGCCAAGGA625920.16590062554650173RNA PCR Primer, Index 1 (100% over 22bp)
AGAACTAGTACCGCAAGGGAAAGATGAAAGACTTGGAATTCTCGGGTGCC598300.1585799211791794No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGG594590.15759658254041164Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA584370.15488776289399478No Hit
TAATCCACCTATAACTTCTCTGTTAACCCTGGAATTCTCGGGTGCCAAGG581920.1542383883212236Illumina Small RNA Adapter 2 (100% over 21bp)
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAA572620.15177341545315345No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA548400.14535388396232993No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA531110.14077115483448768RNA PCR Primer, Index 1 (100% over 23bp)
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA529430.14032586941315886RNA PCR Primer, Index 1 (100% over 22bp)
AGGCATACGCGTATAACAACTCGGATAACCTGGAATTCTCGGGTGCCAAG523150.13866135010009645No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATGGAATTCTCGGGTGCC521950.1383432890848616No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGG494110.1309642735314129No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCTGGTGCCAAG480110.12725356168700622No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG476800.12637624338665007Illumina Small RNA Adapter 2 (100% over 21bp)
AACGGACCAAGTTACCCTAGGGATAACAGCGCTGGAATTCTCGGGTGCCA458990.12165568781887275No Hit
ACGTACACCCTCTAACCTAGAGAAGGTTATTAGTGGAATTCTCGGGTGCC457380.12122895595676597No Hit
ATATACGTACACCCTCTAACCTAGAGAAGGTTATGGAATTCTCGGGTGCC456120.12089499189076935No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGGAATTCTCGGGTGC449310.11908999562931155No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG438700.11627780615294334No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA433530.11490749327897316No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG428670.113619346167272No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA422770.11205554617570061RNA PCR Primer, Index 1 (100% over 23bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAG410250.10873710958341694No Hit
ACGTACACCCTCTAACCTAGAGAAGGTTATGGAATTCTCGGGTGCCAAGG401480.10641261366374218Illumina Small RNA Adapter 2 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTGGAATTCTCGGGTGCCA400880.10625358315612475No Hit
AAAAGAATTACAGCTAGAAACCCCGAAACCTGGAATTCTCGGGTGCCAAG399760.10595672620857223No Hit
CGCCGATGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG394290.10450689808079333No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT387580.1027284069039384No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC383650.10168675707904426No Hit
ATATACGTACACCCTCTAACCTAGAGAAGGTTTGGAATTCTCGGGTGCCA380380.10082004081252928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)