FastQCFastQC Report
Sat 1 Jul 2023
SRR2163140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29928258
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4161261.3904116971993492No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG3796951.2686839307520004Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG2161660.7222805951485717RNA PCR Primer, Index 1 (100% over 45bp)
CCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT1843030.6158159957054634RNA PCR Primer, Index 1 (100% over 46bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1807150.6038273260007314RNA PCR Primer, Index 1 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1735330.5798299386486176No Hit
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1640730.5482210157370335RNA PCR Primer, Index 1 (100% over 45bp)
AGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA1463930.48914641139487636RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1458680.4873922164129967No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCTGGAATTCTCGGGTGCCAAGG1359890.4543832788396839Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1213090.4053326458225534No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1192820.39855978253061036RNA PCR Primer, Index 1 (100% over 44bp)
GAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA1010490.3376374261408733RNA PCR Primer, Index 1 (100% over 40bp)
TACCTGGTTGATCCTGCCAGTAGCTGGAATTCTCGGGTGCCAAGGAACTC982920.32842539649317376RNA PCR Primer, Index 1 (100% over 26bp)
AGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCAAGGAACTC974560.32563204981726634RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC960550.3209508552084789RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG949830.3173689561216694No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC883350.29515583566541026RNA PCR Primer, Index 1 (100% over 24bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG878650.2935854134911561RNA PCR Primer, Index 1 (100% over 45bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG861060.28770802497091547RNA PCR Primer, Index 1 (100% over 45bp)
AGTCAGCGGAGGAAAAGAAACTAACCTGGAATTCTCGGGTGCCAAGGAAC848730.28358817275632947RNA PCR Primer, Index 1 (100% over 24bp)
AGAGCGGCAAGAGTGAGATTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA834160.27871986401614157RNA PCR Primer, Index 1 (100% over 23bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG821510.2744930894407553RNA PCR Primer, Index 1 (100% over 39bp)
AGGGAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC794550.26548488054333136RNA PCR Primer, Index 1 (100% over 42bp)
AACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA785740.2625411742975485RNA PCR Primer, Index 1 (100% over 34bp)
AGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT779710.2605263560612181RNA PCR Primer, Index 1 (100% over 41bp)
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT766780.25620602442013163RNA PCR Primer, Index 1 (100% over 46bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC759740.2538537324825254RNA PCR Primer, Index 1 (100% over 44bp)
GGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC746550.24944652642328863RNA PCR Primer, Index 1 (100% over 44bp)
GGTCCGAAGGTAGTGAGTTATCTCTGGAATTCTCGGGTGCCAAGGAACTC711590.23776525850585759RNA PCR Primer, Index 1 (100% over 26bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC704510.2353996012731513RNA PCR Primer, Index 1 (100% over 44bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT695970.2325461107692937RNA PCR Primer, Index 1 (100% over 46bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC673210.22494125785737346RNA PCR Primer, Index 1 (100% over 26bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC660090.2205574410645618RNA PCR Primer, Index 1 (100% over 44bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA656860.2194781934852339RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC655470.21901374948050767RNA PCR Primer, Index 1 (100% over 50bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA633620.21171295703211324RNA PCR Primer, Index 1 (100% over 40bp)
GAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAA624810.20876925078633043RNA PCR Primer, Index 1 (100% over 23bp)
AACTCGGATAACCATTGTTAGTTAATCTGGAATTCTCGGGTGCCAAGGAA611210.20422505045231834RNA PCR Primer, Index 1 (100% over 23bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT606400.20261787371653908RNA PCR Primer, Index 1 (100% over 43bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG606260.20257109518368893RNA PCR Primer, Index 1 (100% over 45bp)
GCATTGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG605650.2023672744334134Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA574480.1919523682267107No Hit
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC534470.17858373180290013RNA PCR Primer, Index 1 (100% over 44bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC524360.17520565346636613RNA PCR Primer, Index 1 (100% over 44bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG516520.17258605562675916No Hit
AGAGAAGTCGGGCTGAAGCTGAGTCCTTGGAATTCTCGGGTGCCAAGGAA513700.17164380232220666RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA511880.17103568139515504RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA502820.168008442054997No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG493540.16490769359178875RNA PCR Primer, Index 1 (100% over 45bp)
ATTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTC472930.1580212252914954RNA PCR Primer, Index 1 (100% over 26bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG471690.15760690114339432RNA PCR Primer, Index 1 (100% over 45bp)
GACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC468450.15652431224029145RNA PCR Primer, Index 1 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC462480.15452954194661112RNA PCR Primer, Index 1 (100% over 24bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA444120.1483948714956948No Hit
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG431200.14407788117838333RNA PCR Primer, Index 1 (100% over 45bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG430590.1438740604281078Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA412140.13770931806321637RNA PCR Primer, Index 1 (100% over 28bp)
CGAGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC408660.13654653738951328RNA PCR Primer, Index 1 (100% over 42bp)
CTAGCATTACAAGTATTAGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC405580.13551740966681053RNA PCR Primer, Index 1 (100% over 27bp)
AGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC398830.13326201611867955RNA PCR Primer, Index 1 (100% over 44bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG398260.13307156066350403No Hit
CCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG393150.1313641442144745RNA PCR Primer, Index 1 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGAACTC391940.13095984403769842RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAA389800.1302448007498465RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA389250.13006102794222102No Hit
TGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAAC381500.12747150201658913RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG377440.12611492456393553No Hit
CGCCTTAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG370110.12366573423685401RNA PCR Primer, Index 1 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC369920.12360224908512885RNA PCR Primer, Index 1 (100% over 27bp)
TCCCATATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC367570.12281703799800175RNA PCR Primer, Index 1 (100% over 24bp)
GCAGGACACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT364870.12191488057874936RNA PCR Primer, Index 1 (100% over 41bp)
GGAATAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA361200.1206886147533211RNA PCR Primer, Index 1 (100% over 40bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC359900.12025424266256994RNA PCR Primer, Index 1 (100% over 44bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA353310.11805231029483909No Hit
GCATGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA345690.11550622157828232RNA PCR Primer, Index 1 (100% over 22bp)
CGGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT343240.11468759725340512RNA PCR Primer, Index 1 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTCATCTGGAATTCTCGGGTGCCAAGGAACTCC333330.11137634539237132RNA PCR Primer, Index 1 (100% over 27bp)
GGCAGGATCAACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAGGAACT332150.11098206918692026RNA PCR Primer, Index 1 (100% over 25bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG330750.11051428385841904RNA PCR Primer, Index 1 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA321170.10731329568196049RNA PCR Primer, Index 1 (100% over 22bp)
AAAACTATTTGCCAGAGAACTACTAGCCTGGAATTCTCGGGTGCCAAGGA319360.10670851607868388RNA PCR Primer, Index 1 (100% over 22bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG318350.106371042377408No Hit
AGAGGCTGAGATCTGAGATCGACCTGGAATTCTCGGGTGCCAAGGAACTC317190.10598344881950696RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA316270.10567604703220616RNA PCR Primer, Index 1 (100% over 28bp)
CGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC313340.10469703916612853RNA PCR Primer, Index 1 (100% over 44bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTTGGAATTCTCGGGTGCCAAGG307360.10269892754867323Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCTGTAGTGTAGTGGTTATCACGCTTGGAATTCTCGGGTGCCAAGGA301120.10061394151306768RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT300180.10029985707821684RNA PCR Primer, Index 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph