FastQCFastQC Report
Sat 1 Jul 2023
SRR2163141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21964135
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA11874965.406522952076191RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5748702.617312268386622No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA5682182.587026532117017No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG4291861.9540309691230728Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA3356401.5281275588590217RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA2852181.298562406395699RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2002430.9116817029216039No Hit
AGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCAAGGAACTC1516680.6905257138512397RNA PCR Primer, Index 1 (100% over 26bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG1327430.6043625209916075Illumina Small RNA Adapter 2 (100% over 21bp)
GAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAA1254970.5713723759210185RNA PCR Primer, Index 1 (100% over 23bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1195670.5443738166788722RNA PCR Primer, Index 2 (100% over 45bp)
AGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA1177280.5360010763000683RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG1165410.5305968115748696No Hit
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1111970.5062662381195526RNA PCR Primer, Index 2 (100% over 45bp)
AGAGCGGCAAGAGTGAGATTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1089960.49624535635025013RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1073720.48885148447685284RNA PCR Primer, Index 2 (100% over 45bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC981640.44692859518483197RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA907550.4131963312008417RNA PCR Primer, Index 1 (100% over 22bp)
GGTCCGAAGGTAGTGAGTTATCTCTGGAATTCTCGGGTGCCAAGGAACTC861040.39202090134667267RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG844160.38433564536003806No Hit
GCATTGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG835070.3801970803767141Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA783710.3568135052894184RNA PCR Primer, Index 2 (100% over 36bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC775270.3529708772961011RNA PCR Primer, Index 2 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCTGGAATTCTCGGGTGCCAAGG723640.3294643745360334Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA694330.31611989272511754No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC680100.3096411490823563RNA PCR Primer, Index 2 (100% over 44bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC679410.3093270005852723RNA PCR Primer, Index 1 (100% over 31bp)
GAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA557010.253599788928633RNA PCR Primer, Index 2 (100% over 40bp)
AGTCAGCGGAGGAAAAGAAACTAACCTGGAATTCTCGGGTGCCAAGGAAC556220.2532401116638556RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG555110.2527347423424597Illumina Small RNA Adapter 2 (100% over 21bp)
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG534040.24314183098947442RNA PCR Primer, Index 2 (100% over 35bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA527600.2402097783500238No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG512180.2331892423717119No Hit
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT500290.2277758718929746RNA PCR Primer, Index 2 (100% over 37bp)
AGGGAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC492620.224283815410896RNA PCR Primer, Index 2 (100% over 42bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC488180.22226233812531201RNA PCR Primer, Index 2 (100% over 34bp)
AGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT485000.22081452331266402RNA PCR Primer, Index 2 (100% over 41bp)
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT481880.21939402576063205RNA PCR Primer, Index 2 (100% over 46bp)
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT480190.2186245895866147RNA PCR Primer, Index 2 (100% over 39bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG479160.2181556432793734RNA PCR Primer, Index 2 (100% over 45bp)
GCATGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA475420.21645286736764274RNA PCR Primer, Index 1 (100% over 22bp)
ATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC473180.2154330229713121RNA PCR Primer, Index 2 (100% over 42bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG461610.21016534454919347RNA PCR Primer, Index 2 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC458290.20865378946177482RNA PCR Primer, Index 1 (100% over 27bp)
GGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC445040.20262122774240823RNA PCR Primer, Index 2 (100% over 44bp)
ATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTGCCAAGG444630.20243455979486558Illumina Small RNA Adapter 2 (100% over 21bp)
CTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG442390.20141471539853492RNA PCR Primer, Index 2 (100% over 38bp)
GAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAA432090.19672525232612165RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA403950.1839134570972178RNA PCR Primer, Index 1 (100% over 22bp)
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT386250.1758548652154979RNA PCR Primer, Index 2 (100% over 41bp)
ATTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTC369500.16822879662686466RNA PCR Primer, Index 1 (100% over 26bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC365940.166607972496982RNA PCR Primer, Index 2 (100% over 44bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT365840.1665624437292887RNA PCR Primer, Index 2 (100% over 43bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT353200.16080760749285142RNA PCR Primer, Index 2 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC351290.15993800802990876RNA PCR Primer, Index 2 (100% over 44bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG349010.15889995212650077No Hit
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC346660.15783002608570745RNA PCR Primer, Index 2 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG339530.1545838249491728RNA PCR Primer, Index 2 (100% over 45bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC337760.15377796576100083RNA PCR Primer, Index 2 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA337750.15377341288423152RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG330250.15035875530723153Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA328860.14972590543629422No Hit
AACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC325030.14798215363363956RNA PCR Primer, Index 2 (100% over 34bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA320600.14596522922482494RNA PCR Primer, Index 2 (100% over 40bp)
CTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT318980.14522766318819294RNA PCR Primer, Index 2 (100% over 39bp)
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT317150.14439448673940494RNA PCR Primer, Index 1 (100% over 25bp)
AACTCGGATAACCATTGTTAGTTAATCTGGAATTCTCGGGTGCCAAGGAA309050.140706656556245RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGAA299970.13657264444969036RNA PCR Primer, Index 1 (100% over 23bp)
AGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA299170.1362084143081437RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC290700.13235212768451843RNA PCR Primer, Index 2 (100% over 50bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA281720.12826364434565712RNA PCR Primer, Index 1 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGTGCCAAG279540.12727111720994247No Hit
TGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA274970.12519045252635716RNA PCR Primer, Index 2 (100% over 40bp)
TACCTGGTTGATCCTGCCAGTAGCTGGAATTCTCGGGTGCCAAGGAACTC272290.12397028155217585RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC268420.12220831824244387RNA PCR Primer, Index 1 (100% over 24bp)
GACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC262880.11968602451223324RNA PCR Primer, Index 2 (100% over 44bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG258970.11790584969542392RNA PCR Primer, Index 2 (100% over 45bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC258630.1177510518852666RNA PCR Primer, Index 1 (100% over 33bp)
TTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT257280.11713641352140661RNA PCR Primer, Index 2 (100% over 37bp)
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC257030.11702259160217326RNA PCR Primer, Index 1 (100% over 24bp)
TGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAAC256040.11657185680200928RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG252710.11505574883782128No Hit
CAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT249070.11339850169378397RNA PCR Primer, Index 1 (100% over 30bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC248970.11335297292609066RNA PCR Primer, Index 2 (100% over 44bp)
GGCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAA235280.1071200846288734RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG230960.10515324186452142Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG229280.10438835856727342No Hit
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG227350.10350965335079208RNA PCR Primer, Index 2 (100% over 45bp)
AGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT227330.10350054759725343RNA PCR Primer, Index 2 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph