FastQCFastQC Report
Sat 1 Jul 2023
SRR2163142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25647369
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG12695304.949942428792599No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG4994841.9475058045914964Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA3083531.202279266929875No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2342010.9131579929309708No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG2129860.8304399566286896RNA PCR Primer, Index 1 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1895990.7392532154077871RNA PCR Primer, Index 1 (100% over 45bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1887720.7360287131206323RNA PCR Primer, Index 1 (100% over 45bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA1345890.5247672772985018No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1319020.5142905691418095Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1262950.4924286775770255No Hit
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT1103410.4302234665863778RNA PCR Primer, Index 1 (100% over 46bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1098350.4282505546670303No Hit
GAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA1052130.4102292129847705RNA PCR Primer, Index 1 (100% over 40bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1017360.39667226685123136No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1008140.39307735619977235RNA PCR Primer, Index 1 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA972530.37919289109147997No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA958200.37360557334360495No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG916540.35736219181000595RNA PCR Primer, Index 1 (100% over 39bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC894070.3486010592353547RNA PCR Primer, Index 1 (100% over 44bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC848990.33102420759026RNA PCR Primer, Index 1 (100% over 36bp)
GGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC830130.32367062679996533RNA PCR Primer, Index 1 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG789170.3077001777453274RNA PCR Primer, Index 1 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG788690.3075130240454684RNA PCR Primer, Index 1 (100% over 45bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC787540.3070646349728894RNA PCR Primer, Index 1 (100% over 44bp)
GCATTGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG712150.27766980698877924No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG705360.2750223619428566RNA PCR Primer, Index 1 (100% over 45bp)
AGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT700530.273139127838025RNA PCR Primer, Index 1 (100% over 41bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT620460.2419195512802892RNA PCR Primer, Index 1 (100% over 46bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCTGGAATTCTCGGGTGCCAAGG610890.23818817438934964Illumina Small RNA Adapter 2 (100% over 21bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC610310.23796203033535332RNA PCR Primer, Index 1 (100% over 44bp)
CCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT610200.23791914094580227RNA PCR Primer, Index 1 (100% over 41bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG599790.23386024508010939No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT588560.22948162831049065RNA PCR Primer, Index 1 (100% over 35bp)
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA582580.22715000513308012RNA PCR Primer, Index 1 (100% over 37bp)
GCATTGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG582530.22713050995601147Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG565560.22051384685891173No Hit
CTCGTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT556850.21711778701355294RNA PCR Primer, Index 1 (100% over 41bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA547410.21343709758299187RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC538310.20988897535649756RNA PCR Primer, Index 1 (100% over 50bp)
AGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA536710.20926512969030078RNA PCR Primer, Index 1 (100% over 22bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC534020.20821628916400742RNA PCR Primer, Index 1 (100% over 44bp)
AGGGAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC526680.20535439717032966RNA PCR Primer, Index 1 (100% over 42bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA505190.19697537006622395No Hit
TCCCACATGGTCTAGCGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC500520.19515452052801208RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG499390.19471392952626057No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA492320.19195731148875347RNA PCR Primer, Index 1 (100% over 22bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG466380.18184321362553796RNA PCR Primer, Index 1 (100% over 45bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC463470.18070859432014255RNA PCR Primer, Index 1 (100% over 44bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC459270.17907099944637594No Hit
GACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC456560.17801436084925515RNA PCR Primer, Index 1 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGTGGAATTCTCGGGTGCCAAG453610.1768641454022048No Hit
CTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC451760.17614282385066476RNA PCR Primer, Index 1 (100% over 38bp)
GCATGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG446520.17409972929387027Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC442590.17256740837627438RNA PCR Primer, Index 1 (100% over 42bp)
CCGTCTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC440860.1718928752496991RNA PCR Primer, Index 1 (100% over 42bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT430450.1678339793840062RNA PCR Primer, Index 1 (100% over 43bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG418540.1631902282062538RNA PCR Primer, Index 1 (100% over 45bp)
AGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT413080.16106135487035728RNA PCR Primer, Index 1 (100% over 46bp)
TTGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC411070.16027764875219755RNA PCR Primer, Index 1 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC409990.15985655292751472RNA PCR Primer, Index 1 (100% over 24bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG406200.15837881850571106No Hit
AGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC385830.15043648336794313RNA PCR Primer, Index 1 (100% over 44bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC377950.14736404346192392RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA369410.14403426721859852No Hit
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC368110.14352739261481362RNA PCR Primer, Index 1 (100% over 44bp)
CGGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT364960.1422991964594887RNA PCR Primer, Index 1 (100% over 43bp)
TGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGAAC362960.1415193893767427RNA PCR Primer, Index 1 (100% over 24bp)
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT361820.14107489933957748RNA PCR Primer, Index 1 (100% over 41bp)
GCATGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA355770.13871598291427084RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA345610.1347545629339212No Hit
ATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC343240.1338304915408672RNA PCR Primer, Index 1 (100% over 42bp)
GGTCCGAAGGTAGTGAGTTATCTCTGGAATTCTCGGGTGCCAAGGAACTC342030.1333587082558059RNA PCR Primer, Index 1 (100% over 26bp)
GAAGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC341810.13327292947670383RNA PCR Primer, Index 1 (100% over 42bp)
CGCCTTAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG337770.13169771916955694RNA PCR Primer, Index 1 (100% over 39bp)
CTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG334270.13033305677475146RNA PCR Primer, Index 1 (100% over 39bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC333940.13020438860609834RNA PCR Primer, Index 1 (100% over 33bp)
CGAGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC333820.1301576001811336RNA PCR Primer, Index 1 (100% over 42bp)
TTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA321630.12540467601179678RNA PCR Primer, Index 1 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA316910.12356433129651623No Hit
TGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA311720.12154073191679036RNA PCR Primer, Index 1 (100% over 40bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG301230.11745064376778765No Hit
GGAATAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA299340.11671372607459267RNA PCR Primer, Index 1 (100% over 40bp)
GGTTCCATAGTGTAGCGGTTATCACGTCTGTGGAATTCTCGGGTGCCAAG295860.11535686175061464No Hit
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG292270.1139571080370856RNA PCR Primer, Index 1 (100% over 45bp)
GGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG290830.11339564693750848RNA PCR Primer, Index 1 (100% over 45bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA287560.11212066235721878RNA PCR Primer, Index 1 (100% over 40bp)
GAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG286670.11177364820539681RNA PCR Primer, Index 1 (100% over 45bp)
AGAGCGGCAAGAGTGAGATTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA284570.11095485076851351RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA282230.11004247648170071No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG277290.10811635298731812No Hit
CGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC270360.10541432144560325RNA PCR Primer, Index 1 (100% over 44bp)
GGCAGGATCAACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAGGAACT270240.10536753302063848RNA PCR Primer, Index 1 (100% over 25bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG266210.10379622174890532No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA264060.10295792913495337No Hit
CCGATAACGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC258400.10075107509078221RNA PCR Primer, Index 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)