FastQCFastQC Report
Sat 1 Jul 2023
SRR2163143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15410310
Total Bases770.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG10091796.54872614502888No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA9719806.3073358031084386RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2552371.6562742735220772Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1971491.2793318239542228No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAA1956611.26967595071092RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1899551.2326487916206745Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1584241.0280390206296954No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1347590.8744729989208524No Hit
AGAGCGGCAAGAGTGAGATTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1093380.7095120085189721RNA PCR Primer, Index 1 (100% over 23bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1010220.6555481362801916RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG999790.6487799401829035No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA942500.6116035303637629No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA744420.4830662069744217No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG696650.4520674794991146RNA PCR Primer, Index 7 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG695830.45153536820479273RNA PCR Primer, Index 7 (100% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA620030.4023475192906567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG619380.4019257237524748No Hit
GCATTGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG610590.3962217502438302Illumina Small RNA Adapter 2 (100% over 21bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG583030.37833761942491745RNA PCR Primer, Index 7 (100% over 45bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA569280.3694150215018387No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG523800.3399023121533571Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC523610.3397790180729654No Hit
GCATTGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG523050.3394156249939164No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG503020.3264178332557878No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA466650.3028167506039788No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG426820.2769704178566168RNA PCR Primer, Index 7 (100% over 45bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG419250.27205812212732905Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC394720.2561402074325565RNA PCR Primer, Index 7 (100% over 44bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA378300.245485003221869RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA373430.24232478126656765No Hit
GCATGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA367940.23876223125946203RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA359940.23357090155876165RNA PCR Primer, Index 1 (100% over 23bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG339070.22002802020205953RNA PCR Primer, Index 7 (100% over 45bp)
AGGGAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC332710.21590091309000273RNA PCR Primer, Index 7 (100% over 42bp)
AGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT321130.20838646334823893RNA PCR Primer, Index 7 (100% over 41bp)
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT318610.20675119449251833RNA PCR Primer, Index 1 (100% over 25bp)
GCATGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG313460.20340927599769246Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG309630.20092392690348215Illumina Small RNA Adapter 2 (100% over 21bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC304640.1976858350026703RNA PCR Primer, Index 7 (100% over 44bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAAC302010.195979185363565RNA PCR Primer, Index 1 (100% over 24bp)
GAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA300520.1950123002068096RNA PCR Primer, Index 7 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGTGGAATTCTCGGGTGCCAAG299700.1944801889124878No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA292500.18980799218185748RNA PCR Primer, Index 7 (100% over 40bp)
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT282400.18325393843472323RNA PCR Primer, Index 7 (100% over 46bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA280110.18176792030789776No Hit
GGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC278660.18082699179964584RNA PCR Primer, Index 7 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGGAATTCTCGGGTGCCA267550.17361753267779817No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC266830.17315031300473516RNA PCR Primer, Index 7 (100% over 39bp)
AGAGCGGCAAGAGTGAGATTTCTGAGCTTGGAATTCTCGGGTGCCAAGGA266200.17274149579080497RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG265350.17218991701010558No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA264940.17192386136294469No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC258090.16747878530671997No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGTGGAATTCTCGGGTGCCAAG256730.1665962592576009No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA255220.16561639577659373RNA PCR Primer, Index 1 (100% over 22bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG241770.15688847271729123No Hit
GCATTGTTGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG241520.15672624366414434Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGCTTCTGTCGTGAGTGGCACACGCATGGAATTCTCGGGTGCCAAGGA238800.1549611915659062RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCTGGAATTCTCGGGTGCCAAGG238320.1546497117838642Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGAAGTCGGGCTGAAGCTGAGTCCTTGGAATTCTCGGGTGCCAAGGAA232140.15063940959007316RNA PCR Primer, Index 1 (100% over 23bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG226020.14666804236903735No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT220620.1431638948210646RNA PCR Primer, Index 7 (100% over 46bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA218360.14169734418061675No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG216630.14057471913284028Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA214650.13928986503191695No Hit
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG211010.13692781001809826RNA PCR Primer, Index 7 (100% over 45bp)
AGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAGGA198810.1290110322245302RNA PCR Primer, Index 1 (100% over 22bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC198020.12849838841658606RNA PCR Primer, Index 7 (100% over 44bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC196130.1272719367747956RNA PCR Primer, Index 7 (100% over 44bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC195990.12718108850503332RNA PCR Primer, Index 7 (100% over 44bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG192200.12472169605932651Illumina Small RNA Adapter 2 (100% over 21bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC191590.12432585716964811RNA PCR Primer, Index 7 (100% over 44bp)
ATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC190760.12378725671320044RNA PCR Primer, Index 7 (100% over 42bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG190660.12372236509194169RNA PCR Primer, Index 7 (100% over 45bp)
AAGCAGAACCTGGATCCGTTGTTTGAACTGGAATTCTCGGGTGCCAAGGA190190.12341737447202554RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTTGGAATTCTCGGGTGCCA185040.12007545597719968No Hit
GCAGGACACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT180960.11742787782984249RNA PCR Primer, Index 7 (100% over 41bp)
GGTTCCATAGTGTAGCGGTTATCACGTCTGTGGAATTCTCGGGTGCCAAG178490.11582505478475126No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC178020.11552006416483511RNA PCR Primer, Index 7 (100% over 50bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA175850.11411191598352012RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGG175400.11381990368785572Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATGGAATTCTCGGGTGCCAA165680.10751243810150476No Hit
AGCAGAACAAGGTCCTGGACACCAAGTTGGAATTCTCGGGTGCCAAGGAA163040.10579929930027365RNA PCR Primer, Index 1 (100% over 23bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG161140.10456635849635731RNA PCR Primer, Index 7 (100% over 45bp)
GGAATAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA158270.10270396896623106RNA PCR Primer, Index 7 (100% over 40bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA157660.10230813007655265RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT155450.10087402524673417RNA PCR Primer, Index 7 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)