FastQCFastQC Report
Sat 1 Jul 2023
SRR2163144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47462955
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT32155026.774761495570599RNA PCR Primer, Index 5 (100% over 43bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC14492703.0534761268024715RNA PCR Primer, Index 5 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG12215242.5736366393537864No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG7614251.6042511470261385RNA PCR Primer, Index 5 (100% over 45bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT6223351.3112015465535174RNA PCR Primer, Index 5 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG6009491.2661432479288321RNA PCR Primer, Index 5 (100% over 45bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG5648761.190140816137554RNA PCR Primer, Index 5 (100% over 45bp)
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT5051231.0642468426165206RNA PCR Primer, Index 5 (100% over 46bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG5011951.0559709145795915RNA PCR Primer, Index 5 (100% over 45bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT4723790.9952583019746664RNA PCR Primer, Index 5 (100% over 41bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4652380.9802128839217871No Hit
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA4116410.8672890257254315RNA PCR Primer, Index 5 (100% over 36bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT4064300.8563099368760331RNA PCR Primer, Index 5 (100% over 46bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG3626010.7639663396432017RNA PCR Primer, Index 5 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG3538840.7456004372252001RNA PCR Primer, Index 5 (100% over 45bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC3142270.6620468531721213RNA PCR Primer, Index 5 (100% over 44bp)
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG3008520.6338669811013663RNA PCR Primer, Index 5 (100% over 45bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC2802310.5904204658138121RNA PCR Primer, Index 5 (100% over 44bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2467320.5198412108980572No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC2459590.5182125723103418RNA PCR Primer, Index 5 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC2287010.48185158298719494RNA PCR Primer, Index 5 (100% over 44bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC2273610.4790283285143119RNA PCR Primer, Index 5 (100% over 44bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG2158980.45487686133322297Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA2092740.4409207138493589No Hit
TTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT1961880.41334973770596456RNA PCR Primer, Index 5 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC1857120.39127778706572314RNA PCR Primer, Index 1 (100% over 27bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1820530.3835686168296938RNA PCR Primer, Index 5 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1747600.3682029490157113RNA PCR Primer, Index 5 (100% over 50bp)
CGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT1739730.36654481373947323RNA PCR Primer, Index 5 (100% over 46bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1670610.3519818772345717No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1650210.3476837883355556RNA PCR Primer, Index 1 (100% over 28bp)
GAAGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC1632980.34405358874094544RNA PCR Primer, Index 5 (100% over 42bp)
CGACTCTTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG1572470.3313046985801874RNA PCR Primer, Index 5 (100% over 39bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1440190.30343454173892037RNA PCR Primer, Index 5 (100% over 45bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1403170.295634774531RNA PCR Primer, Index 5 (100% over 45bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA1401960.29537983886591135RNA PCR Primer, Index 5 (100% over 40bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1295360.2729202174622292RNA PCR Primer, Index 5 (100% over 44bp)
AGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT1278300.2693258352751109RNA PCR Primer, Index 5 (100% over 46bp)
CGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1277800.26922048995895853RNA PCR Primer, Index 5 (100% over 45bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1274750.26857788353042916RNA PCR Primer, Index 5 (100% over 44bp)
CGAGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT1212970.2555614162666442RNA PCR Primer, Index 5 (100% over 43bp)
CTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1199060.2526307095712857RNA PCR Primer, Index 5 (100% over 45bp)
GAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1176410.24785856674958395RNA PCR Primer, Index 5 (100% over 44bp)
CGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT1160990.24460971719944533RNA PCR Primer, Index 5 (100% over 46bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT1110180.23390452617204302RNA PCR Primer, Index 5 (100% over 43bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1094400.23057982799427468RNA PCR Primer, Index 5 (100% over 44bp)
ACGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC1088420.22931989801309255RNA PCR Primer, Index 5 (100% over 42bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT1083590.2283022622590608No Hit
CGCGACCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC1070630.22557171166439172RNA PCR Primer, Index 5 (100% over 42bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1069750.22538630390796358RNA PCR Primer, Index 5 (100% over 44bp)
CGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1047890.22078060668578262RNA PCR Primer, Index 5 (100% over 44bp)
AGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT1010730.2129513427893396RNA PCR Primer, Index 5 (100% over 46bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC997000.21005856040779594RNA PCR Primer, Index 5 (100% over 44bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT933040.19658278756558667RNA PCR Primer, Index 5 (100% over 43bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG928210.19556515181155493RNA PCR Primer, Index 5 (100% over 37bp)
GACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC927580.19543241671320294RNA PCR Primer, Index 5 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTCATCTGGAATTCTCGGGTGCCAAGGAACTCC921550.19416195220040555RNA PCR Primer, Index 1 (100% over 27bp)
AGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC915980.19298840537846834RNA PCR Primer, Index 5 (100% over 44bp)
CGAGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC896610.18890732783072609RNA PCR Primer, Index 5 (100% over 42bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG881740.18577435812835505Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT863350.18189975740027145RNA PCR Primer, Index 5 (100% over 43bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC847600.17858137994147227RNA PCR Primer, Index 5 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC821210.17302125415495095RNA PCR Primer, Index 5 (100% over 44bp)
CCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT818700.17249242066786613RNA PCR Primer, Index 5 (100% over 43bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA801150.1687948000709185RNA PCR Primer, Index 5 (100% over 40bp)
GAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG801020.16876741028871883RNA PCR Primer, Index 5 (100% over 45bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG800750.1687105238179966No Hit
GGAATAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG791500.16676163546917802RNA PCR Primer, Index 5 (100% over 39bp)
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG782780.16492441315548093RNA PCR Primer, Index 5 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC775370.16336319557010304RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT767040.16160814260300482RNA PCR Primer, Index 5 (100% over 43bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC766920.16158285972712824No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG766870.161572325195513No Hit
ACTAATCCTAGCCCTAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC755960.15927369039706862RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA715270.15070068856858995No Hit
GCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC712180.15004965451476843RNA PCR Primer, Index 5 (100% over 44bp)
CGACTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC693850.14618769522462308RNA PCR Primer, Index 5 (100% over 42bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC691290.14564832720592302RNA PCR Primer, Index 5 (100% over 44bp)
CCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTA685580.1444452836954631RNA PCR Primer, Index 5 (100% over 47bp)
CCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC683220.143948053803224RNA PCR Primer, Index 5 (100% over 44bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG673860.14197598948485193RNA PCR Primer, Index 5 (100% over 39bp)
CTGAACGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT663800.13985644172386655RNA PCR Primer, Index 5 (100% over 41bp)
AGGGAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC663780.13985222791122043RNA PCR Primer, Index 5 (100% over 42bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC662470.13957622318290128RNA PCR Primer, Index 1 (100% over 31bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC651060.13717224306830453RNA PCR Primer, Index 5 (100% over 44bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC645690.13604083437282824RNA PCR Primer, Index 5 (100% over 44bp)
GAGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT644400.13576904345715515RNA PCR Primer, Index 5 (100% over 43bp)
GGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC642040.13527181356491605RNA PCR Primer, Index 5 (100% over 42bp)
GCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT635900.13397817308256513RNA PCR Primer, Index 5 (100% over 43bp)
CCGTCTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC627720.1322547237103126RNA PCR Primer, Index 5 (100% over 42bp)
CGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG621920.13103271804294528RNA PCR Primer, Index 5 (100% over 45bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT610210.12856553073865712RNA PCR Primer, Index 5 (100% over 43bp)
GCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC606710.12782811352559065RNA PCR Primer, Index 5 (100% over 42bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC586610.12359323181626597RNA PCR Primer, Index 5 (100% over 44bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC581750.12256927534326507RNA PCR Primer, Index 5 (100% over 44bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA578030.12178550619109155RNA PCR Primer, Index 1 (100% over 22bp)
CCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG571820.1204771173644793RNA PCR Primer, Index 5 (100% over 45bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC571460.1204012687368496RNA PCR Primer, Index 5 (100% over 44bp)
CGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC567190.1195016197369085RNA PCR Primer, Index 5 (100% over 44bp)
CTCGTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT563970.11882319590088733RNA PCR Primer, Index 5 (100% over 41bp)
TCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC562030.11841445607421619RNA PCR Primer, Index 5 (100% over 44bp)
GGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG560440.1180794579688517RNA PCR Primer, Index 5 (100% over 45bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG556880.11732939931784693No Hit
AGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC551490.11619377680972456RNA PCR Primer, Index 5 (100% over 44bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC551470.11618956299707846RNA PCR Primer, Index 5 (100% over 44bp)
GAGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC549590.11579346460834561RNA PCR Primer, Index 5 (100% over 42bp)
GAAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT535800.11288804078886366RNA PCR Primer, Index 5 (100% over 43bp)
CGAGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT526430.11091386956416853RNA PCR Primer, Index 5 (100% over 41bp)
TTGGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT522430.11007110703494967RNA PCR Primer, Index 5 (100% over 41bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC515740.10866158670483116RNA PCR Primer, Index 5 (100% over 44bp)
GGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC514840.10847196513575692RNA PCR Primer, Index 5 (100% over 44bp)
GGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT511770.10782514489458146RNA PCR Primer, Index 5 (100% over 46bp)
GAGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT510440.1075449263536162RNA PCR Primer, Index 5 (100% over 43bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC507710.10696974092742435RNA PCR Primer, Index 5 (100% over 42bp)
GGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG507190.1068601817986259RNA PCR Primer, Index 5 (100% over 45bp)
CTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC505830.1065736425386915RNA PCR Primer, Index 5 (100% over 44bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA505080.10641562456446295No Hit
CCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT504650.10632502759257192RNA PCR Primer, Index 5 (100% over 41bp)
CCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC493870.10405378257632716RNA PCR Primer, Index 5 (100% over 44bp)
AAAGGTTTGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT487510.1027137901548692RNA PCR Primer, Index 5 (100% over 38bp)
TTGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC483700.10191105884578826RNA PCR Primer, Index 5 (100% over 44bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA480000.10113150350626082RNA PCR Primer, Index 1 (100% over 22bp)
TCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG478340.100781757056635RNA PCR Primer, Index 5 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph