FastQCFastQC Report
Sat 1 Jul 2023
SRR2163145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2163145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25760601
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG7287172.8288043435011474No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4407351.710887878741649No Hit
TACCTGGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA4084321.5854909596247384RNA PCR Primer, Index 7 (100% over 40bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT3701791.4369967532977976RNA PCR Primer, Index 7 (100% over 43bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG2728571.0592027724818998RNA PCR Primer, Index 7 (100% over 45bp)
TACCTGGTTGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA2293340.8902509689117889RNA PCR Primer, Index 7 (100% over 37bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG2084400.809142612782986Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT2020070.7841703693170823RNA PCR Primer, Index 7 (100% over 43bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1717650.6667740399379657RNA PCR Primer, Index 7 (100% over 44bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1636830.6354005483024251No Hit
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1556390.6041745687532679RNA PCR Primer, Index 7 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1543580.5992018586833436RNA PCR Primer, Index 7 (100% over 44bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1505620.5844661776330451No Hit
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT1466040.5691016292671122RNA PCR Primer, Index 7 (100% over 41bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1409790.5472659585853606RNA PCR Primer, Index 7 (100% over 44bp)
CCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1295700.5029773955972533RNA PCR Primer, Index 7 (100% over 44bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT1242880.48247321558996237RNA PCR Primer, Index 7 (100% over 46bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1218010.4728189377258706RNA PCR Primer, Index 7 (100% over 45bp)
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT1113460.43223370448538834RNA PCR Primer, Index 7 (100% over 46bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1065430.41358895314593014RNA PCR Primer, Index 7 (100% over 45bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1019890.395910794161984Illumina Small RNA Adapter 2 (100% over 21bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT1010280.3921802911352883RNA PCR Primer, Index 7 (100% over 38bp)
AGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT992970.385460727410824RNA PCR Primer, Index 7 (100% over 46bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT945410.3669984252308399RNA PCR Primer, Index 7 (100% over 41bp)
CTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT923370.3584427242206034RNA PCR Primer, Index 7 (100% over 38bp)
TCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA916830.35590396357600507RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG845470.32820274651200876RNA PCR Primer, Index 7 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC833560.3235794071729926RNA PCR Primer, Index 7 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA832410.32313298901683235RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG830000.32219745183740084RNA PCR Primer, Index 7 (100% over 45bp)
ATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC820890.3186610436612096RNA PCR Primer, Index 7 (100% over 42bp)
TCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA769870.29885560511573467RNA PCR Primer, Index 7 (100% over 37bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT743280.28853364096590756RNA PCR Primer, Index 7 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC710670.2758747748160068RNA PCR Primer, Index 7 (100% over 42bp)
TACCTGGTTGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT703820.27321567536409574RNA PCR Primer, Index 7 (100% over 38bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA692920.26898440762309855No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA660700.2564769354565912No Hit
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG657790.2553473034266553RNA PCR Primer, Index 7 (100% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG626280.24311544594786436No Hit
TCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC604620.2347072570240112RNA PCR Primer, Index 7 (100% over 42bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG596370.23150469199068763Illumina Small RNA Adapter 2 (100% over 21bp)
CGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT580100.22518884555527258RNA PCR Primer, Index 7 (100% over 46bp)
GTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG577550.2241989618176998RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG554500.21525118920944428No Hit
CGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA546510.21214955349838305RNA PCR Primer, Index 7 (100% over 35bp)
CGACTCTTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC514220.19961490805280513RNA PCR Primer, Index 7 (100% over 39bp)
GATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC510250.19807379493979976RNA PCR Primer, Index 7 (100% over 39bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC501310.19460337901278002RNA PCR Primer, Index 7 (100% over 44bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC497980.19331070730842032RNA PCR Primer, Index 7 (100% over 44bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG490970.19058949750434784RNA PCR Primer, Index 7 (100% over 45bp)
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA474020.1840096820722467No Hit
TTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTC469960.1824336318861505RNA PCR Primer, Index 1 (100% over 26bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG469220.18214637150740387RNA PCR Primer, Index 7 (100% over 45bp)
TAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT449610.17453397147061903RNA PCR Primer, Index 7 (100% over 41bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA438730.170310467523642RNA PCR Primer, Index 7 (100% over 37bp)
CACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG409850.1590995489585045RNA PCR Primer, Index 1 (100% over 29bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT404490.1570188521610967RNA PCR Primer, Index 1 (100% over 25bp)
GGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT404420.1569916788820261RNA PCR Primer, Index 7 (100% over 38bp)
CGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA402070.15607943308465513RNA PCR Primer, Index 7 (100% over 37bp)
ATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA401000.15566407010457559RNA PCR Primer, Index 7 (100% over 40bp)
CCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTA391030.15179381878551668RNA PCR Primer, Index 7 (100% over 47bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC384520.14926670383194865RNA PCR Primer, Index 1 (100% over 27bp)
TTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCC383390.14882804946980857RNA PCR Primer, Index 1 (100% over 27bp)
GTAGTCGTGGCCGAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA354080.1374502093332372RNA PCR Primer, Index 1 (100% over 32bp)
TCACTGACCCGGTGAGGCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCC354060.13744244553921703RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG347770.13500073231987098RNA PCR Primer, Index 7 (100% over 45bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC344420.1337002968214911RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC344060.13356054852912785No Hit
TCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT342470.13294332690452368RNA PCR Primer, Index 7 (100% over 41bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG340490.13217471129652605RNA PCR Primer, Index 7 (100% over 45bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT332250.12897602816021259RNA PCR Primer, Index 7 (100% over 38bp)
CCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC331860.12882463417681908RNA PCR Primer, Index 7 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA322700.12526881651557742No Hit
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC322180.12506695787105276RNA PCR Primer, Index 7 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC320100.12425952329295424RNA PCR Primer, Index 7 (100% over 44bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA317600.12328904904043193RNA PCR Primer, Index 7 (100% over 40bp)
CGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT315560.12249714205037375RNA PCR Primer, Index 7 (100% over 46bp)
AGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG315250.122376803243061RNA PCR Primer, Index 7 (100% over 45bp)
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA307330.11930234081107036RNA PCR Primer, Index 1 (100% over 28bp)
CCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT306670.11904613560840448RNA PCR Primer, Index 7 (100% over 43bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC291660.11321940819626064RNA PCR Primer, Index 2 (100% over 34bp)
TTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG290400.11273028917298941RNA PCR Primer, Index 7 (100% over 36bp)
TCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT289270.11229163481084933RNA PCR Primer, Index 7 (100% over 46bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG284970.110622419096511Illumina Small RNA Adapter 2 (100% over 21bp)
CTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC282900.10981886641542253RNA PCR Primer, Index 7 (100% over 39bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG282740.10975675606326112RNA PCR Primer, Index 7 (100% over 36bp)
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG275230.10684145140868415RNA PCR Primer, Index 7 (100% over 45bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA274780.10666676604323011RNA PCR Primer, Index 7 (100% over 37bp)
TCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC273780.10627857634222121RNA PCR Primer, Index 7 (100% over 42bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG272670.10584768577410131No Hit
CTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC271790.10550607883721347RNA PCR Primer, Index 7 (100% over 42bp)
CGCGACCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC269510.10462100631891313RNA PCR Primer, Index 7 (100% over 42bp)
TACCTGGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC268600.10426775369099502RNA PCR Primer, Index 7 (100% over 39bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC266050.1032778699534223RNA PCR Primer, Index 7 (100% over 44bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGTGGAATTCTCGGGTGCCAAGGA261300.10143396887362993RNA PCR Primer, Index 1 (100% over 22bp)
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG259350.10067699895666254RNA PCR Primer, Index 7 (100% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA258430.10031986443173435No Hit

[FAIL]Adapter Content

Adapter graph