FastQCFastQC Report
Sun 24 Sept 2023
SRR21883627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21883627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48926336
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length10-150
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGA556770911.379779184772797No Hit
AGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGA38600167.889444245324236No Hit
CACCGACCTACTCGACCCTTCTGCCGGC38399527.848435656412121No Hit
AAGCGCTGCAGCGCTGAAGTAGGAGCCCCGTGGA24355994.978094006467192No Hit
TGGCTGTAGTTTAGTGGTGAGAATTCCACGT17709733.619672235419386No Hit
AGCGCTGCAGCGCTGAAGTAGGAGCCCCGTGGA12873052.63110853017892No Hit
ACCGACCTACTCGACCCTTCTGCCGGC10671442.1811238838730946No Hit
CACCGACCTACTCGACCCTTCTGCCGGCG10030962.050216881149653No Hit
GCCGCCGCGTGCCGGCCGGGGGACGGACCGGGA9027121.8450431276930281No Hit
CACCGACCTACTCGACCCTTCTGCCGG8868521.81262704814029No Hit
CACCGACCTACTCGACCCTTCTGCCGGCGA8849641.8087681857067734No Hit
GCACCGACCTACTCGACCCTTCTGCCGGC7382851.5089725909579659No Hit
TCCACGAGGCTCATACCAGGCGCTACGGCGCT6427421.313693304154229No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC6137041.2543428553489067No Hit
GCGCTGCAGCGCTGAAGTATGAGCCCCGTGGA5553331.1350390104830248No Hit
ACCGACCTACTCGACCCTTCTGCCGGCGA5495771.123274385394402No Hit
AGCACCGACCTACTCGACCCTTCTGCCGGC4803580.9817984326478075No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGA3825220.7818325083652289No Hit
CTCCACGAGGCTCATACCAGGCGCTACGGCGCT3044200.6222006896244999No Hit
GCACCGACCTACTCGACCCTTCTGCCGGCG2975200.6080978555189581No Hit
ACCGACCTACTCGACCCTTCTGCCGGCG2793200.5708990757043405No Hit
AAGCGCTGCAGCGCTGAAGTAGGAGCCCCGTGG2655360.5427261097172696No Hit
AAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGG2466580.5041415731601074No Hit
AAGCGCTGCAGCGCTGAAGTAGGAGCCCCGTGGAC2198830.44941644516360263No Hit
GCGCTGCAGCGCTGAAGTAGGAGCCCCGTGGA1939270.3963652622587557No Hit
GGCTGTAGTTTAGTGGTGAGAATTCCACGT1923880.39321971708651965No Hit
GCCGCCGCGTGCCGGCCGGGGGACGGACCGGGAA1881670.3845924616141294No Hit
CGGCCCCCTCGGGGGCCTTCCCCGGGC1638650.33492187111661087No Hit
GGTTCCATGGTGTAGTGGTTAGCACTCCAGA1605740.3281954324149677No Hit
AAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGAC1600740.3271734879145661No Hit
GCCCCTCTTTTTGGATCCAAGGCTGACT1554960.3178165640688892No Hit
AGCACCGACCTACTCGACCCTTCTGCCGGCGA1523860.31146006927639136No Hit
AGCGCTGCAGCGCTGAAGTATGAGCCCCGTGG1513390.30932011749255045No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGAA1451550.29668070791158363No Hit
GGCCGCCGCGTGCCGGCCGGGGGACGGACCGGGA1374350.2809018848253832No Hit
TGCCGGCCGGGGGACGGACCGGGAACGGCCCCC1301930.2661000406815667No Hit
AGCGCTGCAGCGCTGAAGTAGGAGCCCCGTGG1212670.24785628746039762No Hit
TTTACTAAAGAATACCGTGTGCGCGCCACA1168120.2387507619618195No Hit
GGGCCGCCGCGTGCCGGCCGGGGGACGGACCGGGA1158540.23679271629905005No Hit
GCACCGACCTACTCGACCCTTCTGCCGGCGA1142610.23353680112077063No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGC1118910.2286927841888671No Hit
AGCGCTGAAGTATGAGCCCCGTGGA1076840.22009414316248818No Hit
GGAGGCAGCGGGGATTCGACTTCCCCTGGGGG1061640.21698743188126737No Hit
TCCACGAGGCTCATACCAGGCGCTACGGCGCTG1054250.21547699790967384No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATACCTGGC911040.1862064635291717No Hit
TGGCTGTAGTTTAGTGGTGAGAATTCCACG862240.17623228520525225No Hit
AGCACCGACCTACTCGACCCTTCTGCCGG841670.17202800553060013No Hit
TTTACTAAAGAATACCGTGTGCGCGCCGCA820130.16762546862287012No Hit
GGCGCGTCGAGAGAACCATGTCGAAGGAACT817180.16702252136763315No Hit
TACTAAAGAATACCGTGTGCGCGCCACA752150.15373111119541016No Hit
AGATGGTTATAGGTTTAAGGACACAAGGTGAC742980.15185686498167367No Hit
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGC737600.15075725269924156No Hit
TCCACGAGGCTCATACCAGGCGCTACGGCGC725140.14821056700424085No Hit
AGCGCTGCAGCGCTGAAGTAGGAGCCCCGTGGAC689540.14093432216138155No Hit
GGAGGCAGCGGGGATTCGACTTCCCCTGGGGGT688580.14073810881730445No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATACCTGGCT679580.1388986087165816No Hit
TCCAGTGCAGGGTGGCCCGAGGTTGTCTGACAGAC670750.1370938547288724No Hit
AGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGAC669320.13680157860175754No Hit
ATCAGAGTGGCGCAGCGGAAGCGTGGTGGGC653150.13349661008745883No Hit
CCAATGATCACCCATGACTTCTGTGTACCA648170.13247875336505885No Hit
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGC632020.12917787262876174No Hit
AGATGGTTATAGGTTTAAGGACACAAGGTGACC607610.1241887395778012No Hit
TGCCCCTCTTTTTGGATCCAAGGCTGACT560320.11452318849300303No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC560040.11446595960098055No Hit
GAGGCAGCGGGGATTCGACTTCCCCTGGGGG553920.113215099532489No Hit
GGGCGCGTCGAGAGAACCATGTCGAAGGAACT540950.1105641754984473No Hit
ACCGACCTACTCGACCCTTCTGCCGG525250.10735526976718632No Hit
GGGTCCATAGCTCAGTGGTAGAGCA509540.10414432014692455No Hit
GGGTCCATAGCTCAGTGGTAGAGCAATTGA506250.1034718806656603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)