FastQCFastQC Report
Sun 24 Sept 2023
SRR21883628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21883628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59798885
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length10-150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGACCTACTCGACCCTTCTGCCGGC41323166.910356271693026No Hit
AAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGA23184613.877097373972776No Hit
CACCGACCTACTCGACCCTTCTGCCGGCGA18716013.129825915650434No Hit
GCACCGACCTACTCGACCCTTCTGCCGGC16396382.741920689658344No Hit
AGCACCGACCTACTCGACCCTTCTGCCGGC14810132.4766565463553376No Hit
TGGCTGTAGTTTAGTGGTGAGAATTCCACGT14654222.4505841538684208No Hit
AGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGA11612421.9419124620801875No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC10618561.7757120387779806No Hit
CACCGACCTACTCGACCCTTCTGCCGGCG10582981.769762095062475No Hit
AAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGAC8468921.416233764224868No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGC8148101.3625839344663366No Hit
GCACCGACCTACTCGACCCTTCTGCCGGCG7459611.2474496807089297No Hit
ACCGACCTACTCGACCCTTCTGCCGGC7452101.2461938044496983No Hit
ACCGACCTACTCGACCCTTCTGCCGGCGA6513211.0891858602380295No Hit
CACCGACCTACTCGACCCTTCTGCCGG4901390.8196457174744981No Hit
CACGTGTGAACCGAAGTCGTCTTGCCGAAC4666170.7803105358904266No Hit
AGCACCGACCTACTCGACCCTTCTGCCGGCGA4054920.6780929109296938No Hit
GCACCGACCTACTCGACCCTTCTGCCGGCGA3969610.6638267586427405No Hit
ATATCGGGTAGGTTGTGGTATTTCATTGCTA3939370.6587698081661556No Hit
ACCGACCTACTCGACCCTTCTGCCGGCG3423650.5725273974589994No Hit
AGCGCTGCAGCGCTGAAGTATGAGCCCCGTGGAC3252870.5439683365333651No Hit
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGC3111220.520280603894203No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGC3100030.5184093315452286No Hit
AGATGGTTATAGGTTTAAGGACACAAGGTGACC2891700.48357088932343806No Hit
TTTACTAAAGAATACCGTGTGCGCGCCACA2335330.39053069300539633No Hit
GGGTCCATAGCTCAGTGGTAGAGCATTTGA2315240.387171098591554No Hit
GGGTCCATAGCTCAGTGGTAGAGCAATTGA2251570.3765237428758078No Hit
AGCACCGACCTACTCGACCCTTCTGCCGG2156000.360541839534299No Hit
CGGCCCCCTCGGGGGCCTTCCCCGGGC2100500.35126073002866187No Hit
GTCGTTGTAGTATAGTGGTGAGTATTCCCGC2051030.3429880005287724No Hit
TCCATTGTCGTCTAGTCCGGTTAGGATACCTGGC1923820.32171502863305895No Hit
CGTATGGCTGCGGGCCTGGGTTGGCAGTGC1869890.3126964658287525No Hit
GGTTCCATGGTGTAGTGGTTAGCACTCCAGA1779450.2975724380145215No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGACT1712330.2863481484646411No Hit
AAGCGCTGCAGCGCTGAAGTATGAGCCCCGTGG1678440.280680818714262No Hit
CAGGATAGCTCAGTTGGTAGAGCAGAGGACT1610180.26926589015831986No Hit
TCACGTGTGAACCGAAGTCGTCTTGCCGAAC1429260.23901114544192587No Hit
TTTACTAAAGAATACCGTGTGCGCGCCGCA1356440.2268336608617368No Hit
GATGGTTATAGGTTTAAGGACACAAGGTGACC1314870.21988202622841546No Hit
AAGATGGTTATAGGTTTAAGGACACAAGGTGACC1279040.2138902757133348No Hit
TGGCTGTAGTTTAGTGGTGAGAATTCCACG1248670.20881158570097086No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGT1210630.20245026307764766No Hit
TGAAATGATGTGAGCATTCCTTGAGCTGG1174400.1963916216832471No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC1164210.19468757653257246No Hit
CACCGACCTGCTCGACCCTTCTGCCGGC1129760.18892659955114546No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGC1127180.18849515337953207No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGA1076630.18004181850547882No Hit
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCC1064980.17809362164528653No Hit
TCAGGATAGCTCAGTTGGTAGAGCAGAGGAC1030170.17227244287247162No Hit
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGC1010330.16895465525820422No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTC976760.16334083821128104No Hit
AGCACCGACCTACTCGACCCTTCTGCCGGCG976030.1632187623565222No Hit
TTTACTAAAGAATACCGTGTGCTCGCTGCA946090.15821198003942716No Hit
AGCGCTGCAGCGCTGAAGTATGAGCCCCGTGG943300.1577454161561708No Hit
CAGGATAGCTCAGTTGGTAGAGCAGAGGAC913960.15283897015805561No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACT889950.1488238451268782No Hit
TTTACTAAAGAATACCGTGTGCACGCTGCA888580.14859474386520752No Hit
AGGTGTGAAGCGATCTCGTACTAAACGAA879890.14714153951198253No Hit
TGCCCGCTGATCCGATGATTCATGATAACTC815020.1362935111582766No Hit
TGGCCCCTCCTTTTGGCTCTAAGGCCCGAGT785900.13142385514378738No Hit
GCACCGACCTACTCGACCCTTCTGCCGG777940.13009272664532123No Hit
GCGAGCGTAGTTCAATGGTAAAACATCTCCTT765830.12806760527391106No Hit
AGCGCTGAAGTATGAGCCCCGTGGA752130.12577659265720423No Hit
ACGTGTGAACCGAAGTCGTCTTGCCGAAC747150.12494380120967138No Hit
GCGCTGCAGCGCTGAAGTATGAGCCCCGTGGA720750.12052900317455753No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACC719210.12027147328917587No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCG703980.11772460305907041No Hit
TTTAAGGACACAAGGTGACC668210.11174288617588773No Hit
AGGGATATAACTCAGCGGTAGAGTGTCACC660910.11052212762829942No Hit
CACGGGCCGCACCGCTGGCCGACCCTGA657000.10986826928294734No Hit
ATGGTTATAGGTTTAAGGACACAAGGTGACC651100.1088816288129787No Hit
GGGCCTGTAGCTCAGAGG640980.10718928956618505No Hit
TGCTTAACGGCCCGCCAACCCTGGAA636150.10638158219839719No Hit
GTTTAAGGACACAAGGTGACC634570.10611736322508353No Hit
ACCAATGATCACCCATGACTTCTGTGTACCA633380.10591836285910683No Hit
TTTTACTAAAGAATACCGTGTGCGCGCCACA625810.10465245296797758No Hit
TACTAAAGAATACCGTGTGCGCGCCACA618210.10338152626089933No Hit
GTGGCTGTAGTTTAGTGGTGAGAATTCCACGT613550.10260224751682244No Hit
GAGCACCGACCTACTCGACCCTTCTGCCGGCG604280.10105205138858359No Hit
GCACCAGTAGTCTAGTGGTAGAATAGTACC603060.10084803420665786No Hit
ATGGCTGCGGGCCTGGGTTGGCAGTGCC601370.10056542024153126No Hit
CGGCGAGCACCGACCTACTCGACCCTTCTGCCGGC598500.10008547818241091No Hit

[OK]Adapter Content

Adapter graph